دورية أكاديمية
أعرض في EDS

Decoding the universal human chromatin landscape through teratoma-based profiling.

التفاصيل البيبلوغرافية
العنوان: Decoding the universal human chromatin landscape through teratoma-based profiling.
المؤلفون: Kidder BL; Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA.; Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, MI, USA.
المصدر: Nucleic acids research [Nucleic Acids Res] 2024 Apr 24; Vol. 52 (7), pp. 3589-3606.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Teratoma*/genetics , Teratoma*/pathology , Teratoma*/metabolism , Chromatin*/metabolism , Chromatin*/genetics, Humans ; Epigenesis, Genetic ; Transcriptome/genetics ; Gene Expression Profiling/methods ; Cell Differentiation/genetics ; Histone Code ; Single-Cell Analysis/methods ; Epigenome ; Human Embryonic Stem Cells/metabolism ; RNA-Seq
مستخلص: Teratoma formation is key for evaluating differentiation of human pluripotent stem cells into embryonic germ layers and serves as a model for understanding stem cell differentiation and developmental processes. Its potential for insights into epigenome and transcriptome profiling is significant. This study integrates the analysis of the epigenome and transcriptome of hESC-generated teratomas, comparing transcriptomes between hESCs and teratomas. It employs cell type-specific expression patterns from single-cell data to deconvolve RNA-Seq data and identify cell types within teratomas. Our results provide a catalog of activating and repressive histone modifications, while also elucidating distinctive features of chromatin states. Construction of an epigenetic signature matrix enabled the quantification of diverse cell populations in teratomas and enhanced the ability to unravel the epigenetic landscape in heterogeneous tissue contexts. This study also includes a single cell multiome atlas of expression (scRNA-Seq) and chromatin accessibility (scATAC-Seq) of human teratomas, further revealing the complexity of these tissues. A histology-based digital staining tool further complemented the annotation of cell types in teratomas, enhancing our understanding of their cellular composition. This research is a valuable resource for examining teratoma epigenomic and transcriptomic landscapes and serves as a model for epigenetic data comparison.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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معلومات مُعتمدة: P30 CA022453 United States CA NCI NIH HHS
المشرفين على المادة: 0 (Chromatin)
تواريخ الأحداث: Date Created: 20240128 Date Completed: 20240424 Latest Revision: 20240508
رمز التحديث: 20240508
مُعرف محوري في PubMed: PMC11039989
DOI: 10.1093/nar/gkae021
PMID: 38281248
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkae021