دورية أكاديمية
Efficacy of novel staphylococcal surface associated protein vaccines against Staphylococcus aureus and non-aureus staphylococcal mastitis in dairy cows.
| العنوان: | Efficacy of novel staphylococcal surface associated protein vaccines against Staphylococcus aureus and non-aureus staphylococcal mastitis in dairy cows. |
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| المؤلفون: | Vidlund J; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA; East Tennessee AgResearch and Education Center-Little River Animal and Environmental Unit, University of Tennessee, Walland, TN 37886, USA., Gelalcha BD; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Gillespie BE; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Agga GE; Food Animal Environmental Systems Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Bowling Green, KY 42101, USA., Schneider L; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Swanson SM; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Frady KD; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA., Kerro Dego O; Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA. Electronic address: okerrode@utk.edu. |
| المصدر: | Vaccine [Vaccine] 2024 Feb 27; Vol. 42 (6), pp. 1247-1258. Date of Electronic Publication: 2024 Jan 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam, The Netherlands : Elsevier Science Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983- |
| مواضيع طبية MeSH: | Staphylococcal Vaccines* , Mastitis, Bovine* , Staphylococcal Infections*/prevention & control , Staphylococcal Infections*/veterinary , Vaccines*, Female ; Pregnancy ; Cattle ; Animals ; Humans ; Staphylococcus aureus ; Milk ; Lactation ; Membrane Proteins |
| مستخلص: | Mastitis is an inflammation of the mammary gland commonly caused by bacteria or fungi. Staphylococcus aureus is a major bacterium that causes mastitis in dairy cows. Non-aureus staphylococci are also increasingly reported, with Staphylococcus chromogenes being the most common species. Current staphylococcal mastitis control programs are not fully effective, and treatment with antibiotics is not sustainable. Non-antibiotic sustainable control tools, such as effective vaccines, are critically needed. We previously developed S. aureus surface-associated proteins (SASP) and S. chromogenes surface-associated proteins (SCSP) vaccines that conferred partial protective effects. We hypothesized that vaccination with SASP or SCSP would reduce the incidence of S. aureus mastitis throughout the lactation period. The objective of this study was to evaluate the efficacy of SASP and SCSP vaccines against S. aureus and non-aureus staphylococcal mastitis under natural exposure over 300 days of lactation. Pregnant Holstein dairy cows (n = 45) were enrolled and assigned to receive SASP (n = 15) or SCSP (n = 16) vaccines or unvaccinated control (n = 14). Cows were vaccinated with 1.2 mg of SASP or SCSP with Emulsigen-D adjuvant. Control cows were injected with phosphate-buffered saline with Emulsigen-D adjuvant. Three vaccine injections were given subcutaneously at 60, 40, and 20 days before the expected calving. Booster vaccinations were given at 120 and 240 days in milk. Cows were monitored for mastitis at quarter and cow levels, staphylococcal mastitis incidence, changes in serum and milk anti-SASP and anti-SCSP antibody titers, bacterial counts in milk, adverse reactions, milk yield and milk somatic cells count over 300 days of lactation. The SCSP vaccine conferred a significant reduction in the incidence of staphylococcal mastitis. Milk and serum anti-SASP and anti-SCSP antibody titers were increased in the vaccinated cows compared to unvaccinated control cows. Anti-SASP and anti-SCSP antibody titers decreased at about 120 days in milk, indicating the duration of immunity of about four months. In conclusion, the SASP and SCSP vaccines conferred partial protection from natural infection. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Mastitis, immunity, intramammary infection; Staphylococcus aureus; Staphylococcus chromogenes; Vaccine |
| المشرفين على المادة: | 0 (Staphylococcal Vaccines) 0 (Vaccines) 0 (Membrane Proteins) |
| تواريخ الأحداث: | Date Created: 20240128 Date Completed: 20240226 Latest Revision: 20240226 |
| رمز التحديث: | 20240226 |
| DOI: | 10.1016/j.vaccine.2024.01.067 |
| PMID: | 38281900 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1873-2518 |
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| DOI: | 10.1016/j.vaccine.2024.01.067 |