دورية أكاديمية
Plum blossom low molecular weight polypeptide protects HaCaT cells against UVB-induced oxidative damage in vitro and underlying mechanism.
| العنوان: | Plum blossom low molecular weight polypeptide protects HaCaT cells against UVB-induced oxidative damage in vitro and underlying mechanism. |
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| المؤلفون: | Chen Z; Huzhou Jiahen Industrial Co., Ltd., Huzhou, China., Cao P; Huzhou Jiahen Industrial Co., Ltd., Huzhou, China., Zhu T; Zhejiang University of Technology, Hangzhou, China., Yan C; Huzhou Jiahen Industrial Co., Ltd., Huzhou, China., Zheng Z; Huzhou Jiahen Industrial Co., Ltd., Huzhou, China., Yao H; Huzhou Jiahen Industrial Co., Ltd., Huzhou, China. |
| المصدر: | Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) [Skin Res Technol] 2024 Feb; Vol. 30 (2), pp. e13582. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Country of Publication: England NLM ID: 9504453 Publication Model: Print Cited Medium: Internet ISSN: 1600-0846 (Electronic) Linking ISSN: 0909752X NLM ISO Abbreviation: Skin Res Technol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1998-> : Oxford : Blackwell Original Publication: Copenhagen, Denmark ; Cambridge, MA : Munksgaard, c1995- |
| مواضيع طبية MeSH: | HaCaT Cells* , Prunus domestica*/metabolism, Humans ; Reactive Oxygen Species ; Ultraviolet Rays/adverse effects ; NF-E2-Related Factor 2/metabolism ; NF-E2-Related Factor 2/pharmacology ; Molecular Weight ; Oxidative Stress ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Superoxide Dismutase/metabolism ; Superoxide Dismutase/pharmacology ; Peptides/metabolism |
| مستخلص: | Background: Increasing amounts of ultraviolet radiation occur as ozone depletion causes the earth's ozone layer to be destroyed, making antioxidant efficacy a research hotspot. Previous studies on plum blossom have mostly focused on Volatile Oils, Flavonoids, Phenylpropanoids, and other compounds, whereas few studies have focused on low molecular weight polypeptide (LMWP) of plum blossom. This research provides a reference for the deep processing and utilization of plum blossom. Objectives: (a) Plum blossom low molecular weight polypeptides protect HaCaT cells against UVB-induced oxidative damage in vitro and the underlying mechanism. (b) Improve the theoretical basis for the intense processing and utilization of plum blossom. Methods: The safe concentration of LMWP and the survival rate of HaCaT cells were determined using the CCK-8 experiment. The fluorescence intensity of reactive oxygen species (ROS) was identified using the dichlorofluorescin diacetate (DCFH-DA) method; Superoxide dismutase (SOD) and malondialdehyde (MDA) concentrations were measured in ruptured cells; Western blot analysis was used to examine the expression levels of three proteins: nuclear factor E2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), and benzoquinone oxidoreductase 1 (NQO-1). Results: It was noted that a certain concentration of LMWP could promote cell proliferation. In oxidatively damaged HaCaT cells, SOD levels and survival rates were markedly reduced, but ROS and MDA levels were elevated. However, after treatment with LMWP, the survival rate of the cells and SOD levels were markedly increased, and the levels of ROS and MDA were markedly decreased. As shown by Western blotting, the model group exhibited lower levels of Nrf2, HO-1, and NQO-1 expression than the control group, whereas LMWP-treated cells had significantly higher levels of Nrf2, HO-1, and NQO-1 expression than their model-treated counterparts. Conclusions: LMMP can effectively protect HaCaT cells against oxidative damage in vitro induced by UVB, and the underlying mechanism is linked to the activation of the transcription factor Nrf2. (© 2024 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd.) |
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| فهرسة مساهمة: | Keywords: HaCaT; LMWP; UVB; antioxidant |
| المشرفين على المادة: | 0 (Reactive Oxygen Species) 0 (NF-E2-Related Factor 2) 0 (Antioxidants) EC 1.15.1.1 (Superoxide Dismutase) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20240129 Date Completed: 20240130 Latest Revision: 20240228 |
| رمز التحديث: | 20240228 |
| مُعرف محوري في PubMed: | PMC10823150 |
| DOI: | 10.1111/srt.13582 |
| PMID: | 38282275 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1600-0846 |
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| DOI: | 10.1111/srt.13582 |