دورية أكاديمية
Overexpression of NDRG1 leads to poor prognosis in hepatocellular carcinoma through mediating immune infiltration and EMT.
| العنوان: | Overexpression of NDRG1 leads to poor prognosis in hepatocellular carcinoma through mediating immune infiltration and EMT. |
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| المؤلفون: | Wang X; Department of Pathology, Tianjin Medical University, Tianjin 300070, China., Sun R; Hospital of Integrated Chinese and Western Medicine , Tianjin 300100, China., Che N; Department of Pathology, Tianjin Medical University, Tianjin 300070, China; Dpartment of Pathology, General Hospital of Tianjin Medical University, Tianjin 300052, China., Zhang D; Department of Pathology, Tianjin Medical University, Tianjin 300070, China; Dpartment of Pathology, General Hospital of Tianjin Medical University, Tianjin 300052, China., Li Y; Department of Pathology, Tianjin Medical University, Tianjin 300070, China; Dpartment of Pathology, General Hospital of Tianjin Medical University, Tianjin 300052, China., Zhao N; Department of Pathology, Tianjin Medical University, Tianjin 300070, China; Dpartment of Pathology, General Hospital of Tianjin Medical University, Tianjin 300052, China. Electronic address: zhaonantj@tmu.edu.cn. |
| المصدر: | Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver [Dig Liver Dis] 2024 Aug; Vol. 56 (8), pp. 1382-1399. Date of Electronic Publication: 2024 Jan 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 100958385 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3562 (Electronic) Linking ISSN: 15908658 NLM ISO Abbreviation: Dig Liver Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : Amsterdam : Elsevier Original Publication: Roma, Italy : Editrice gastroenterologica italiana, c2000- |
| مواضيع طبية MeSH: | Carcinoma, Hepatocellular*/genetics , Carcinoma, Hepatocellular*/pathology , Carcinoma, Hepatocellular*/immunology , Liver Neoplasms*/genetics , Liver Neoplasms*/pathology , Liver Neoplasms*/immunology , Epithelial-Mesenchymal Transition*/genetics , Cell Cycle Proteins*/genetics , Cell Cycle Proteins*/metabolism , Intracellular Signaling Peptides and Proteins*/genetics , Intracellular Signaling Peptides and Proteins*/metabolism, Humans ; Prognosis ; Cell Line, Tumor ; Lymphocytes, Tumor-Infiltrating/immunology ; Gene Expression Regulation, Neoplastic ; Cell Proliferation/genetics ; Male ; Biomarkers, Tumor/metabolism ; Biomarkers, Tumor/genetics ; Cell Movement/genetics ; Female |
| مستخلص: | Background: NDRG1, the first member of the NDRG family, is a multifunctional protein associated with carcinogenesis. Its function in human cancer is currently poorly understood. The aim of this study was to explore the importance of NDRG1 in tumor immune cell infiltration and epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma. Methods: NDRG1 expression in various cancers was analyzed using TIMER 2.0, the Human Protein Atlas (HPA), UALCAN and PrognoScan. Wound healing, Transwell, MTT and colony formation assays were performed to confirm the effects of NDRG1 on the metastasis and proliferation of HCC cells. Western blotting was used to study the effect of NDRG1 on the expression of EMT-related proteins. Signaling networks were constructed using LinkedOmics and Metascape. TIMER2.0 and TISIDB were used for comprehensive analysis of tumor-infiltrating immune cells and tumor-infiltrating lymphocytes (TILs). Result: NDRG1 expression was higher in HCC tissue than in normal liver tissue at both the mRNA and protein levels. Overexpression of NDRG1 is associated with poor prognosis in HCC patients. Genomic analysis suggests that NDRG1 promoter hypermethylation leads to enhanced transcription, which may be one mechanism for NDRG1 upregulation in HCC. The overexpression of NDRG1 promotes the invasion, migration, and proliferation of HCC cells and induces the expression of EMT-related proteins. Immunoinfiltration analysis suggests that NDRG1 is involved in the recruitment of immune cells. Conclusions: The present study showed that NDRG1 may induce metastasis and invasion through EMT and immune cell infiltration. NDRG1 could be used as a biomarker for the diagnosis and prognosis of HCC and could be a potential therapeutic target in HCC. Competing Interests: Conflict of interest Written informed consent was obtained from all participants. Ethical approval was obtained from the Ethical Committee of Tianjin medical university. The authors declare that there are no competing interests. (Copyright © 2024 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: EMT; HCC; Immune infiltration; Methylation; NDRG1; Prognosis |
| المشرفين على المادة: | 0 (N-myc downstream-regulated gene 1 protein) 0 (Cell Cycle Proteins) 0 (Intracellular Signaling Peptides and Proteins) 0 (Biomarkers, Tumor) |
| تواريخ الأحداث: | Date Created: 20240130 Date Completed: 20240728 Latest Revision: 20240728 |
| رمز التحديث: | 20240729 |
| DOI: | 10.1016/j.dld.2024.01.182 |
| PMID: | 38290958 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-3562 |
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| DOI: | 10.1016/j.dld.2024.01.182 |