دورية أكاديمية
أعرض في EDS

Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease.

التفاصيل البيبلوغرافية
العنوان: Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease.
المؤلفون: Loor-Giler A; Facultad de Ingeniería y Ciencias Aplicadas, Carrera de Ingeniería en Biotecnología, Universidad de Las Américas (UDLA), Antigua Vía a Nayón S/N, Quito, EC, 170124, Ecuador., Castillo-Reyes S; Facultad de Ciencias de La Salud, de Las Américas (UDLA), Carrera de Medicina Veterinaria, UniversidadAntigua Vía a Nayón S/N, Quito, EC, 170124, Ecuador., Santander-Parra S; Facultad de Ciencias de La Salud, de Las Américas (UDLA), Carrera de Medicina Veterinaria, UniversidadAntigua Vía a Nayón S/N, Quito, EC, 170124, Ecuador., Caza M; Facultad de Ciencias de La Salud, de Las Américas (UDLA), Carrera de Medicina Veterinaria, UniversidadAntigua Vía a Nayón S/N, Quito, EC, 170124, Ecuador., Kyriakidis NC; Facultad de Medicina, Cancer Research Group, Universidad de Las Américas (UDLA), Quito, 170504, Ecuador., Ferreira AJP; Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, Av. Prof. Dr. Orlando M. Paiva, 87, Sao Paulo, SP, 05508-270, Brazil., Nuñez L; Facultad de Ciencias de La Salud, de Las Américas (UDLA), Carrera de Medicina Veterinaria, UniversidadAntigua Vía a Nayón S/N, Quito, EC, 170124, Ecuador. fabiann7@yahoo.es.; One Health Research Group, Universidad de Las Américas, Quito, Ecuador. fabiann7@yahoo.es.
المصدر: BMC veterinary research [BMC Vet Res] 2024 Jan 30; Vol. 20 (1), pp. 33. Date of Electronic Publication: 2024 Jan 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Avastrovirus*/genetics , Astroviridae Infections*/diagnosis , Astroviridae Infections*/veterinary , RNA Viruses*/genetics , Poultry Diseases*, Animals ; Female ; Chickens ; RNA, Viral/genetics ; RNA, Viral/analysis ; Poultry
مستخلص: Background: Enteric viruses are among the most prominent etiological agents of Runting-Stunting Syndrome (RSS). The Avian Nephritis Virus (ANV) is an astrovirus associated with enteric diseases in poultry, whose early diagnosis is essential for maintaining a good poultry breeding environment. ANV is an RNA virus that rapidly mutates, except for some conserved regions such as ORF1b. Therefore, the approach of a diagnostic method based on fast-RT-qPCR using SYBR® Green that focuses on the amplification of a fragment of ORF1b is presented as a feasible alternative for the diagnosis of this viral agent. In this study, the proposed assay showed a standard curve with an efficiency of 103.8% and a LoD and LoQ of 1 gene viral copies. The assay was specific to amplify the ORF 1b gene, and no amplification was shown from other viral genomes or in the negative controls. 200 enteric (feces) samples from chickens (broilers) and laying hens with signs of RSS from Ecuadorian poultry flocks were examined to validate the proposed method.
Results: Using our method, 164 positive results were obtained out of the total number of samples run, while the presence of viral RNA was detected in samples collected from one day to 44 weeks old in both avian lines.
Conclusions: Our study presents a novel, rapid, robust, and sensitive molecular assay capable of detecting and quantifying even low copy numbers of the ANV in commercial birds, therefore introducing a handy tool in the early diagnosis of ANV in enteric disease outbreaks in poultry.
(© 2024. The Author(s).)
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معلومات مُعتمدة: VET.LNN.19.02 Universidad de Las Americas, Quito - Ecuador
فهرسة مساهمة: Keywords: ANV; Enteric viruses; RT-qPCR; SYBR Green; diagnosis
المشرفين على المادة: 0 (RNA, Viral)
تواريخ الأحداث: Date Created: 20240130 Date Completed: 20240201 Latest Revision: 20240201
رمز التحديث: 20240201
مُعرف محوري في PubMed: PMC10826264
DOI: 10.1186/s12917-024-03881-8
PMID: 38291450
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-03881-8