دورية أكاديمية
أعرض في EDS

Comparative genomics of Leishmania donovani progeny from genetic crosses in two sand fly species and impact on the diversity of diagnostic and vaccine candidates.

التفاصيل البيبلوغرافية
العنوان: Comparative genomics of Leishmania donovani progeny from genetic crosses in two sand fly species and impact on the diversity of diagnostic and vaccine candidates.
المؤلفون: Sádlová J; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic., Yeo M; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Mateus DS; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Phelan J; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Hai LA; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Bhattacharyya T; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Kurtev S; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom., Sebesta O; Laboratory of Confocal and Fluorescence Microscopy, Faculty of Science, Charles University, Prague, Czech Republic., Myskova J; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic., Seblova V; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic., Andersson B; Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden., Florez de Sessions P; Genome Institute of Singapore, Biomedical Sciences Institutes, Agency for Science, Technology and Research, Singapore., Volf P; Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic., Miles MA; Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London United Kingdom.
المصدر: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 Jan 31; Vol. 18 (1), pp. e0011920. Date of Electronic Publication: 2024 Jan 31 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101291488 Publication Model: eCollection Cited Medium: Internet ISSN: 1935-2735 (Electronic) Linking ISSN: 19352727 NLM ISO Abbreviation: PLoS Negl Trop Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Phlebotomus*/genetics , Leishmania donovani*/genetics , Psychodidae*/genetics , Leishmaniasis, Cutaneous* , Leishmaniasis, Visceral*/diagnosis , Leishmaniasis, Visceral*/prevention & control , Leishmaniasis, Visceral*/epidemiology, Animals ; Crosses, Genetic ; Genomics
مستخلص: Sand fly transmitted Leishmania species are responsible for severe, wide ranging, visceral and cutaneous leishmaniases. Genetic exchange can occur among natural Leishmania populations and hybrids can now be produced experimentally, with limitations. Feeding Phlebotomus orientalis or Phlebotomus argentipes on two strains of Leishmania donovani yielded hybrid progeny, selected using double drug resistance and fluorescence markers. Fluorescence activated cell sorting of cultured clones derived from these hybrids indicated diploid progeny. Multilocus sequence typing of the clones showed hybridisation and nuclear heterozygosity, although with inheritance of single haplotypes in a kinetoplastid target. Comparative genomics showed diversity of clonal progeny between single chromosomes, and extraordinary heterozygosity across all 36 chromosomes. Diversity between progeny was seen for the HASPB antigen, which has been noted previously as having implications for design of a therapeutic vaccine. Genomic diversity seen among Leishmania strains and hybrid progeny is of great importance in understanding the epidemiology and control of leishmaniasis. As an outcome of this study we strongly recommend that wider biological archives of different Leishmania species from endemic regions should be established and made available for comparative genomics. However, in parallel, performance of genetic crosses and genomic comparisons should give fundamental insight into the specificity, diversity and limitations of candidate diagnostics, vaccines and drugs, for targeted control of leishmaniasis.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Sádlová et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust
تواريخ الأحداث: Date Created: 20240131 Date Completed: 20240202 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10830044
DOI: 10.1371/journal.pntd.0011920
PMID: 38295092
قاعدة البيانات: MEDLINE
الوصف
تدمد:1935-2735
DOI:10.1371/journal.pntd.0011920