دورية أكاديمية
أعرض في EDS

Low prevalence of mcr-1 in Escherichia coli from food-producing animals and food products in China.

التفاصيل البيبلوغرافية
العنوان: Low prevalence of mcr-1 in Escherichia coli from food-producing animals and food products in China.
المؤلفون: Mei CY; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Jiang Y; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Ma QC; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Lu MJ; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Wu H; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Wang ZY; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China., Jiao X; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. jiao@yzu.edu.cn.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China. jiao@yzu.edu.cn., Wang J; Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. wj1231@yzu.edu.cn.; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, No. 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, China. wj1231@yzu.edu.cn.
المصدر: BMC veterinary research [BMC Vet Res] 2024 Feb 01; Vol. 20 (1), pp. 40. Date of Electronic Publication: 2024 Feb 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Escherichia coli* , Escherichia coli Proteins*/genetics, Animals ; Humans ; Swine ; Colistin/pharmacology ; Anti-Bacterial Agents/pharmacology ; Prevalence ; Chickens/genetics ; Plasmids ; China/epidemiology ; Microbial Sensitivity Tests/veterinary ; Drug Resistance, Bacterial/genetics
مستخلص: Background: mcr-1-positive Escherichia coli has emerged as a significant threat to human health, veterinary health, and food safety in recent years. After the prohibition of colistin as a feed additive in animal husbandry in China, a noticeable reduction in both colistin resistance and the prevalence of mcr-1 was observed in E. coli from animals and humans.
Objectives: To assess the prevalence of the colistin resistance gene mcr-1 and characterize its genetic context in E. coli strains derived from fecal and meat samples from food-producing animals in China.
Methods: A total of 1,353 fecal samples and 836 food samples were collected between 2019 and 2020 in China. E. coli isolates were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and their susceptibility to colistin were determined using the broth microdilution method. The colistin-resistant E. coli isolates were screened for the presence of mcr by PCR analysis and sequencing. The minimal inhibitory concentrations (MICs) of 15 antimicrobial agents against the mcr-1-positive strains were further tested using the agar dilution method, conjugation assays were performed, and whole genome sequencing was performed using Illumina HiSeq.
Results: In total, 1,403 E. coli strains were isolated. Thirteen isolates from chicken meat (n = 7), chickens (n = 3), and pigs (n = 3) were resistant to colistin with MIC values of 4 to 16 mg/L, and carried mcr-1. All mcr-1-positive strains, except for isolate AH20PE105, contained multiple resistance genes and exhibited multidrug-resistant phenotypes. They belonged to 10 sequence types (STs), including a novel ST (ST14521). mcr-1 was located on IncI2 (n = 9), IncX4 (n = 2), and IncHI2 (n = 2) plasmids, which were highly similar to other mcr-1-carrying plasmids sharing the same incompatibility type. Seven mcr-1-carrying plasmids could be successfully conjugally transferred to E. coli C600.
Conclusions: While the low prevalence of mcr-1 (0.93%) identified in this study may not immediately seem alarming, the very emergence of this gene merits attention given its implications for colistin resistance and public health. Hence, ongoing surveillance of mcr-1 in E. coli remains crucial.
(© 2024. The Author(s).)
References: J Antimicrob Chemother. 2016 Aug;71(8):2314-7. (PMID: 27246235)
J Antimicrob Chemother. 2007 May;59(5):880-5. (PMID: 17353219)
J Antimicrob Chemother. 2018 May 1;73(5):1121-1137. (PMID: 29370371)
Sci Rep. 2017 Nov 10;7(1):15364. (PMID: 29127343)
Antimicrob Agents Chemother. 2019 Aug 23;63(9):. (PMID: 31209009)
Front Microbiol. 2018 Feb 27;9:331. (PMID: 29535696)
Front Microbiol. 2018 Dec 04;9:2989. (PMID: 30564223)
PLoS One. 2018 Dec 26;13(12):e0209706. (PMID: 30586457)
Lancet Infect Dis. 2016 Feb;16(2):161-8. (PMID: 26603172)
mBio. 2018 Jul 3;9(4):. (PMID: 29970465)
Data Brief. 2020 Oct 22;33:106439. (PMID: 33163593)
Commun Biol. 2019 Jan 25;2:36. (PMID: 30701201)
Clin Microbiol Infect. 2017 Jan;23(1):51.e1-51.e4. (PMID: 27615718)
Front Microbiol. 2022 May 06;13:873856. (PMID: 35602044)
Pathogens. 2022 Jun 08;11(6):. (PMID: 35745513)
Nat Commun. 2018 Mar 21;9(1):1179. (PMID: 29563494)
J Glob Antimicrob Resist. 2022 Dec;31:185-186. (PMID: 36218030)
Int J Antimicrob Agents. 2018 Jun;51(6):822-828. (PMID: 29339296)
Front Microbiol. 2021 Jul 20;12:712707. (PMID: 34354696)
Mol Biol Rep. 2021 Mar;48(3):2897-2907. (PMID: 33839987)
Microbiol Spectr. 2018 Jul;6(4):. (PMID: 30003866)
Int J Antimicrob Agents. 2022 Feb;59(2):106504. (PMID: 34929292)
Clin Infect Dis. 2021 Apr 8;72(7):1211-1219. (PMID: 32406495)
mBio. 2018 Feb 13;9(1):. (PMID: 29440577)
Plasmid. 2015 Jul;80:111-7. (PMID: 25952328)
Vet Microbiol. 2014 May 14;170(1-2):89-96. (PMID: 24553411)
Braz J Microbiol. 2021 Sep;52(3):1597-1609. (PMID: 34114111)
Crit Rev Microbiol. 2019 Mar;45(2):131-161. (PMID: 31122100)
Lancet Infect Dis. 2020 Oct;20(10):1161-1171. (PMID: 32505232)
Antimicrob Agents Chemother. 2021 May 18;65(6):. (PMID: 33820767)
Microbiol Spectr. 2022 Dec 21;10(6):e0277322. (PMID: 36409077)
معلومات مُعتمدة: BE2021331 Key Research and Development Program (Modern Agriculture) project of Jiangsu Province; PAPD Priority Academic Program Development of Jiangsu Higher Education Institutions
فهرسة مساهمة: Keywords: Colistin; Escherichia coli; Plasmids; mcr-1
المشرفين على المادة: Z67X93HJG1 (Colistin)
0 (Escherichia coli Proteins)
0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20240131 Date Completed: 20240202 Latest Revision: 20240203
رمز التحديث: 20240205
مُعرف محوري في PubMed: PMC10832210
DOI: 10.1186/s12917-024-03891-6
PMID: 38297289
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-024-03891-6