دورية أكاديمية
Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.).
| العنوان: | Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.). |
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| المؤلفون: | Andersson E; Institute of Marine Research, NO-5817 Bergen, Norway., Schulz RW; Institute of Marine Research, NO-5817 Bergen, Norway.; Science Faculty, Department Biology, Utrecht University, NL-3584 CH Utrecht, The Netherlands., Almeida F; Embrapa Amazônia Ocidental, 69010-97 Manaus, Amazonas, Brazil., Kleppe L; Institute of Marine Research, NO-5817 Bergen, Norway., Skaftnesmo KO; Institute of Marine Research, NO-5817 Bergen, Norway., Kjærner-Semb E; Institute of Marine Research, NO-5817 Bergen, Norway., Crespo D; Institute of Marine Research, NO-5817 Bergen, Norway., Fjelldal PG; Institute of Marine Research, NO-5817 Bergen, Norway., Hansen TJ; Institute of Marine Research, NO-5817 Bergen, Norway., Norberg B; Institute of Marine Research, NO-5817 Bergen, Norway., Edvardsen RB; Institute of Marine Research, NO-5817 Bergen, Norway., Wargelius A; Institute of Marine Research, NO-5817 Bergen, Norway. |
| المصدر: | Endocrinology [Endocrinology] 2024 Feb 20; Vol. 165 (4). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0375040 Publication Model: Print Cited Medium: Internet ISSN: 1945-7170 (Electronic) Linking ISSN: 00137227 NLM ISO Abbreviation: Endocrinology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Los Angeles, Calif. : Association for the Study of Internal Secretions, |
| مواضيع طبية MeSH: | Receptors, FSH*/genetics , Receptors, FSH*/metabolism , Salmo salar*/genetics , Salmo salar*/metabolism, Male ; Animals ; Mice ; Zebrafish/genetics ; Sexual Maturation/genetics ; Follicle Stimulating Hormone/metabolism ; Testis/metabolism |
| مستخلص: | Early puberty poses a significant challenge for male Atlantic salmon in aquaculture due to its negative impact on growth and welfare. The regulation of puberty in vertebrates involves 2 key reproductive hormones: follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and their gonadal receptors. In male mice lacking FSH receptor, testes size is reduced, but fertility is maintained, while medaka and zebrafish with a disrupted fshr gene exhibit near normal testis size and fertility. In these fishes both Fsh and Lh are present during puberty and Lh may rescue fertility, while in salmonid fish only Fsh is present in the circulation during puberty. Using CRISPR-Cas9, we produced crispants with a high prevalence of fshr mutations at the target site, which remained fertile, although more than half showed a testis development deviating from wild-type (wt) males. Crossing out these F0 crispants to each other produced a viable F1 generation showing frameshift (fshr-/-) or in-frame mutations (fshrif/if). Nearly all wt males matured while all fshr-/- males remained immature with small testes containing A spermatogonia as the furthest developed germ cell type and prepubertal plasma androgen levels. Also, the pituitary transcript levels of gnrhr2bba and lhb, but not for fshb, were reduced in the fshr-/- males compared with maturing males. More than half of the fshrif/if mutant males showed no or a delayed maturation. In conclusion, Atlantic salmon show the unique characteristic that loss of Fshr function alone results in male infertility, offering new opportunities to control precocious puberty or fertility in salmon. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society.) |
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| معلومات مُعتمدة: | 267610 Research Council of Norway |
| فهرسة مساهمة: | Keywords: CRISPR-Cas9; FSH receptor; aquaculture; puberty; sterility |
| المشرفين على المادة: | 0 (Receptors, FSH) 9002-68-0 (Follicle Stimulating Hormone) |
| تواريخ الأحداث: | Date Created: 20240201 Date Completed: 20240221 Latest Revision: 20240330 |
| رمز التحديث: | 20240330 |
| مُعرف محوري في PubMed: | PMC10878062 |
| DOI: | 10.1210/endocr/bqae013 |
| PMID: | 38298132 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1945-7170 |
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| DOI: | 10.1210/endocr/bqae013 |