دورية أكاديمية
MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1.
العنوان: | MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1. |
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المؤلفون: | Li Y; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Xu HL; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Kang XW; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Xu S; Department of Emergency Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Mou ZF; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. |
المصدر: | Heliyon [Heliyon] 2023 Nov 23; Vol. 10 (2), pp. e22772. Date of Electronic Publication: 2023 Nov 23 (Print Publication: 2024). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: London : Elsevier Ltd, [2015]- |
مستخلص: | Purpose: Sepsis-induced acute lung injury is related to high mortality. MiR-2113 possesses important functions in human diseases. This research aimed to clarify the role and mechanism of miR-2113 in sepsis-induced acute lung injury. Methods: The expression of miR-2113 in lipopolysaccharide (LPS)-induced MLE-12 cells, serum of sepsis patients, and cecal ligation and puncture mouse models was examined using quantitative real-time PCR. The functions of miR-2113 in LPS-treated MLE-12 cells were estimated by Cell Counting Kit-8 assay, flow cytometry, enzyme-linked immunosorbent assay, Western blot, and immunofluorescence. The influences of miR-2113 in cecal ligation and puncture-induced acute lung injury in mice were assessed by hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay, acute pulmonary dysfunction analysis, lactate dehydrogenase levels and total protein concentrations in bronchoalveolar lavage fluid, and Masson staining. Also, the mechanism of miR-2113 was examined using a dual-luciferase reporter assay. Results: MiR-2113 expression was decreased in LPS-induced MLE-12 cells, serum of sepsis patients, and cecal ligation and puncture mouse models. miR-2113 overexpression restored LPS-reduced MLE-12 cell proliferation, but alleviated LPS-induced apoptosis and markers of inflammation and fibrosis in MLE-12 cells. Moreover, we found that miR-2113 mimic reduced LPS-induced MLE-12 cell injury by negatively regulating high-mobility group box 1. In vivo data further confirmed that miR-2113 overexpression alleviated acute pulmonary dysfunction, inflammation and fibrosis in cecal ligation and puncture-induced sepsis mice. Conclusion: MiR-2113 relieved sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis through decreasing high-mobility group box 1. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.) |
References: | Oxid Med Cell Longev. 2022 Jun 28;2022:9493710. (PMID: 35799888) Front Immunol. 2020 Aug 04;11:1722. (PMID: 32849610) Toxicol Lett. 2020 May 17;330:134-143. (PMID: 32428545) JAMA. 2016 Feb 23;315(8):801-10. (PMID: 26903338) Am J Physiol Lung Cell Mol Physiol. 2012 Aug 1;303(3):L199-207. (PMID: 22659882) Liver Int. 2015 Apr;35(4):1172-84. (PMID: 25039534) Int J Exp Pathol. 2021 Aug;102(4-5):192-199. (PMID: 34716956) Acta Biochim Pol. 2021 Mar 22;68(2):151-158. (PMID: 33751878) Free Radic Res. 2020 Jun;54(6):408-418. (PMID: 32530324) Crit Care. 2014 Oct 21;18(5):568. (PMID: 25331176) Int J Chron Obstruct Pulmon Dis. 2019 May 24;14:1109-1118. (PMID: 31213791) Crit Care. 2019 Feb 13;23(1):44. (PMID: 30760290) Int J Med Sci. 2020 Sep 16;17(16):2570-2577. (PMID: 33029099) J Transl Med. 2021 Sep 9;19(1):386. (PMID: 34503521) Chin Med J (Engl). 2020 Sep 20;133(18):2212-2218. (PMID: 32858588) Ther Adv Chronic Dis. 2020 Sep 11;11:2040622320956429. (PMID: 32963751) DNA Cell Biol. 2022 May;41(5):479-486. (PMID: 35486848) Immunol Invest. 2022 Jul;51(5):1407-1422. (PMID: 34251977) Front Immunol. 2018 Mar 07;9:357. (PMID: 29536989) J Inflamm Res. 2020 Nov 23;13:961-968. (PMID: 33262632) Int J Mol Sci. 2019 Dec 11;20(24):. (PMID: 31835747) Front Endocrinol (Lausanne). 2021 Mar 19;12:616696. (PMID: 33815277) Mol Med Rep. 2022 Jul;26(1):. (PMID: 35616155) J Transl Med. 2021 Mar 2;19(1):96. (PMID: 33653364) Bioengineered. 2021 Dec;12(1):2132-2139. (PMID: 34057015) Theranostics. 2020 Mar 26;10(11):4749-4761. (PMID: 32308747) J Surg Res. 2020 Dec;256:23-30. (PMID: 32682121) Redox Biol. 2023 Jun;62:102655. (PMID: 36913799) Acta Biochim Pol. 2021 May 10;68(2):201-206. (PMID: 33966370) Mil Med Res. 2022 Oct 9;9(1):56. (PMID: 36209190) Biochem Biophys Res Commun. 2018 Jul 2;501(4):827-832. (PMID: 29654764) J Cell Physiol. 2020 Nov;235(11):8199-8209. (PMID: 31975383) Biomedicines. 2022 Mar 23;10(4):. (PMID: 35453505) Front Cell Dev Biol. 2022 Jul 04;10:941914. (PMID: 35859904) J Thorac Dis. 2018 Jun;10(6):3187-3195. (PMID: 30069314) Cell Signal. 2022 Aug;96:110363. (PMID: 35644425) Nanotoxicology. 2020 Nov;14(9):1175-1197. (PMID: 32924694) Chin J Nat Med. 2019 Sep;17(9):641-649. (PMID: 31526499) Biomed Pharmacother. 2019 Mar;111:852-858. (PMID: 30841464) Arch Pharm Res. 2021 Apr;44(4):414-426. (PMID: 33759138) |
فهرسة مساهمة: | Keywords: Acute pulmonary dysfunction; High-mobility group box 1; Inflammation; MiR-2113; Sepsis; fibrosis |
تواريخ الأحداث: | Date Created: 20240201 Latest Revision: 20240202 |
رمز التحديث: | 20240202 |
مُعرف محوري في PubMed: | PMC10828656 |
DOI: | 10.1016/j.heliyon.2023.e22772 |
PMID: | 38298668 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2405-8440 |
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DOI: | 10.1016/j.heliyon.2023.e22772 |