دورية أكاديمية
أعرض في EDS

MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1.

التفاصيل البيبلوغرافية
العنوان: MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1.
المؤلفون: Li Y; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Xu HL; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Kang XW; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Xu S; Department of Emergency Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China., Mou ZF; Department of Critical Care Medicine, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China.
المصدر: Heliyon [Heliyon] 2023 Nov 23; Vol. 10 (2), pp. e22772. Date of Electronic Publication: 2023 Nov 23 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Ltd Country of Publication: England NLM ID: 101672560 Publication Model: eCollection Cited Medium: Print ISSN: 2405-8440 (Print) Linking ISSN: 24058440 NLM ISO Abbreviation: Heliyon Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: London : Elsevier Ltd, [2015]-
مستخلص: Purpose: Sepsis-induced acute lung injury is related to high mortality. MiR-2113 possesses important functions in human diseases. This research aimed to clarify the role and mechanism of miR-2113 in sepsis-induced acute lung injury.
Methods: The expression of miR-2113 in lipopolysaccharide (LPS)-induced MLE-12 cells, serum of sepsis patients, and cecal ligation and puncture mouse models was examined using quantitative real-time PCR. The functions of miR-2113 in LPS-treated MLE-12 cells were estimated by Cell Counting Kit-8 assay, flow cytometry, enzyme-linked immunosorbent assay, Western blot, and immunofluorescence. The influences of miR-2113 in cecal ligation and puncture-induced acute lung injury in mice were assessed by hematoxylin-eosin staining, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay, acute pulmonary dysfunction analysis, lactate dehydrogenase levels and total protein concentrations in bronchoalveolar lavage fluid, and Masson staining. Also, the mechanism of miR-2113 was examined using a dual-luciferase reporter assay.
Results: MiR-2113 expression was decreased in LPS-induced MLE-12 cells, serum of sepsis patients, and cecal ligation and puncture mouse models. miR-2113 overexpression restored LPS-reduced MLE-12 cell proliferation, but alleviated LPS-induced apoptosis and markers of inflammation and fibrosis in MLE-12 cells. Moreover, we found that miR-2113 mimic reduced LPS-induced MLE-12 cell injury by negatively regulating high-mobility group box 1. In vivo data further confirmed that miR-2113 overexpression alleviated acute pulmonary dysfunction, inflammation and fibrosis in cecal ligation and puncture-induced sepsis mice.
Conclusion: MiR-2113 relieved sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis through decreasing high-mobility group box 1.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Authors.)
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فهرسة مساهمة: Keywords: Acute pulmonary dysfunction; High-mobility group box 1; Inflammation; MiR-2113; Sepsis; fibrosis
تواريخ الأحداث: Date Created: 20240201 Latest Revision: 20240202
رمز التحديث: 20240202
مُعرف محوري في PubMed: PMC10828656
DOI: 10.1016/j.heliyon.2023.e22772
PMID: 38298668
قاعدة البيانات: MEDLINE
الوصف
تدمد:2405-8440
DOI:10.1016/j.heliyon.2023.e22772