دورية أكاديمية
أعرض في EDS

Evaluation of salivary MMP-9 in oral squamous cell carcinoma and oral leukoplakia using ELISA.

التفاصيل البيبلوغرافية
العنوان: Evaluation of salivary MMP-9 in oral squamous cell carcinoma and oral leukoplakia using ELISA.
المؤلفون: Pazhani J; Department of Oral Pathology, Azeezia College of Dental Sciences and Research, Kollam, Kerala, India.; Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India., Chanthu K; Department of Oral Pathology, Azeezia College of Dental Sciences and Research, Kollam, Kerala, India., Jayaraman S; Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India., Varun BR; Department of Oral Pathology, PMS College of Dental Sciences and Research, Trivandrum, Kerala, India.
المصدر: Journal of oral and maxillofacial pathology : JOMFP [J Oral Maxillofac Pathol] 2023 Oct-Dec; Vol. 27 (4), pp. 649-654. Date of Electronic Publication: 2023 Dec 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Medknow Publications Pvt. Ltd Country of Publication: India NLM ID: 101227995 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0973-029X (Print) Linking ISSN: 0973029X NLM ISO Abbreviation: J Oral Maxillofac Pathol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2007- : Mumbai : Medknow Publications Pvt. Ltd.
Original Publication: Chennai, Tamilnadu : [s.n.]
مستخلص: Background: Cancer of the lip and the oral cavity is collectively the sixth most common malignancy worldwide, out of which 90% are oral squamous cell carcinomas (OSCCs). Oral cancer survival rates depend mainly upon the stage in which it is diagnosed. Successful early detection would eventually increase the survival rate. OSCCs may be preceded by potentially malignant disorders (PMDs) that are characterised by visible clinical changes in the oral mucosa. Correct diagnosis and timely treatment of PMDs may help prevent malignant transformation in oral lesions. Oral leukoplakia (OL) is the best known potentially malignant disorder of the oral mucosa with a malignant transformation rate of about 3% to 33%. Tumour markers in saliva have emerged as a new diagnostic tool in the early detection of oral cancer. Matrix metalloproteinase 9 (MMP-9) is a gelatinase which plays an important role in tumourogenisis. The present study was done to evaluate the salivary levels of MMP-9 in OSCC and oral leukoplakia patients using enzyme-linked immunosorbent assay (ELISA).
Materials and Methods: The study was conducted among 102 subjects, which included 34 OSCC patients (group I), 34 OL patients (group II), and 34 healthy subjects (group III). Unstimulated saliva was collected by the passive drooling method from all the study subjects during the study period, centrifuged, and stored at -80°C. The salivary MMP-9 was estimated in mg/ml using the sandwich ELISA technique. The data were analysed using a statistical software package, EZR. One-way analysis of variance was used for the comparison of salivary MMP-9 levels in OSCC, OL, and normal oral mucosa. Scheffe's multiple comparison was carried out to compare salivary MMP-9 levels among the different histological grades of OSCC and oral epithelial dysplasia. For all statistical interpretations, P ≤ 0.0 was considered the threshold for statistical significance.
Results and Conclusion: The mean salivary MMP-9 level in OSCC, OL, and normal oral mucosa was 50.9 ± 5.7 ng/ml, 31.6 ± 6 ng/ml, and 16.2 ± 4.8 ng/ml, respectively. Patients with OSCC had significantly higher levels of salivary MMP-9 when compared to OL and normal mucosa. Higher levels of salivary MMP-9 were observed in poorly differentiated OSCC when compared to well and moderately differentiated OSCCs. The salivary MMP-9 was higher in severe oral epithelial dysplasia when compared to mild and moderate oral epithelial dysplasias. As malignant transformation rates are higher in patients with severe oral epithelial dysplasia when compared to mild and moderate oral epithelial dysplasia, salivary MMP-9 could be considered as a surrogate marker of malignant transformation.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2023 Journal of Oral and Maxillofacial Pathology.)
References: Crit Rev Biochem Mol Biol. 2013 May-Jun;48(3):222-72. (PMID: 23547785)
J Clin Diagn Res. 2016 Aug;10(8):ZC78-83. (PMID: 27656569)
J Oral Biosci. 2019 Jun;61(2):84-94. (PMID: 31109866)
Crit Rev Oral Biol Med. 1993;4(2):197-250. (PMID: 8435466)
Monogr Oral Sci. 2014;24:88-98. (PMID: 24862597)
J Family Med Prim Care. 2018 Nov-Dec;7(6):1173-1176. (PMID: 30613493)
J Oral Maxillofac Pathol. 2012 May;16(2):256-61. (PMID: 22923900)
Oncotarget. 2017 Jan 3;8(1):364-378. (PMID: 27861153)
Contemp Oncol (Pozn). 2017;21(2):178-183. (PMID: 28947890)
Br J Cancer. 2010 Jul 27;103(3):303-9. (PMID: 20628386)
J Dent Res. 2021 Nov;100(12):1313-1320. (PMID: 34036828)
Ann N Y Acad Sci. 1993 Sep 20;694:72-7. (PMID: 8215087)
Indian J Med Sci. 2003 Sep;57(9):400-4. (PMID: 14515030)
J Oral Pathol Med. 2008 Jan;37(1):1-10. (PMID: 18154571)
J Natl Cancer Inst. 2020 Oct 1;112(10):1047-1054. (PMID: 31860085)
Cochrane Database Syst Rev. 2013 Nov 19;(11):CD004150. (PMID: 24254989)
BMC Cancer. 2019 Jun 14;19(1):581. (PMID: 31200666)
Med Oral Patol Oral Cir Bucal. 2017 May 1;22(3):e270-275. (PMID: 28160595)
Clin Microbiol Rev. 2013 Oct;26(4):781-91. (PMID: 24092855)
Int J Clin Pediatr Dent. 2014 May;7(2):86-92. (PMID: 25356006)
Curr Issues Mol Biol. 2022 Dec 12;44(12):6306-6322. (PMID: 36547091)
فهرسة مساهمة: Keywords: ELISA; MMP-9; oral leukoplakia; oral squamous cell carcinoma
تواريخ الأحداث: Date Created: 20240202 Latest Revision: 20240203
رمز التحديث: 20240205
مُعرف محوري في PubMed: PMC10829443
DOI: 10.4103/jomfp.jomfp_426_23
PMID: 38304520
قاعدة البيانات: MEDLINE
الوصف
تدمد:0973-029X
DOI:10.4103/jomfp.jomfp_426_23