CRISPR technologies for genome, epigenome and transcriptome editing.

التفاصيل البيبلوغرافية
العنوان:	CRISPR technologies for genome, epigenome and transcriptome editing.
المؤلفون:	Villiger L; McGovern Institute for Brain Research, Massachusetts Institute of Technology Cambridge, Cambridge, MA, USA., Joung J; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Koblan L; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Weissman J; Whitehead Institute for Biomedical Research, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA.; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA., Abudayyeh OO; McGovern Institute for Brain Research, Massachusetts Institute of Technology Cambridge, Cambridge, MA, USA. omar@abudayyeh.science., Gootenberg JS; McGovern Institute for Brain Research, Massachusetts Institute of Technology Cambridge, Cambridge, MA, USA. jgoot@mit.edu.
المصدر:	Nature reviews. Molecular cell biology [Nat Rev Mol Cell Biol] 2024 Jun; Vol. 25 (6), pp. 464-487. Date of Electronic Publication: 2024 Feb 02.
نوع المنشور:	Journal Article; Review
اللغة:	English
بيانات الدورية:	Publisher: Nature Pub. Group Country of Publication: England NLM ID: 100962782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1471-0080 (Electronic) Linking ISSN: 14710072 NLM ISO Abbreviation: Nat Rev Mol Cell Biol Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: London, UK : Nature Pub. Group, [2000-
مواضيع طبية MeSH:	Gene Editing/methods , CRISPR-Cas Systems/genetics , Epigenome/genetics , Transcriptome/genetics, Humans ; Animals ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Genome/genetics
مستخلص:	Our ability to edit genomes lags behind our capacity to sequence them, but the growing understanding of CRISPR biology and its application to genome, epigenome and transcriptome engineering is narrowing this gap. In this Review, we discuss recent developments of various CRISPR-based systems that can transiently or permanently modify the genome and the transcriptome. The discovery of further CRISPR enzymes and systems through functional metagenomics has meaningfully broadened the applicability of CRISPR-based editing. Engineered Cas variants offer diverse capabilities such as base editing, prime editing, gene insertion and gene regulation, thereby providing a panoply of tools for the scientific community. We highlight the strengths and weaknesses of current CRISPR tools, considering their efficiency, precision, specificity, reliance on cellular DNA repair mechanisms and their applications in both fundamental biology and therapeutics. Finally, we discuss ongoing clinical trials that illustrate the potential impact of CRISPR systems on human health. (© 2024. Springer Nature Limited.)
التعليقات:	Erratum in: Nat Rev Mol Cell Biol. 2024 May 13;:. (PMID: 38740926)
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تواريخ الأحداث:	Date Created: 20240202 Date Completed: 20240528 Latest Revision: 20240529
رمز التحديث:	20240530
DOI:	10.1038/s41580-023-00697-6
PMID:	38308006
قاعدة البيانات:	MEDLINE

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تدمد:	1471-0080
DOI:	10.1038/s41580-023-00697-6