دورية أكاديمية
أعرض في EDS

Progesterone-induced progesterone receptor membrane component 1 rise-to-decline changes are essential for decidualization.

التفاصيل البيبلوغرافية
العنوان: Progesterone-induced progesterone receptor membrane component 1 rise-to-decline changes are essential for decidualization.
المؤلفون: Liu H; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany., Franken A; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany., Bielfeld AP; Department of OB/GYN & REI, UniKiD, University Hospital and Faculty of Medicine, Heinrich Heine University, Duesseldorf, Germany., Fehm T; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany., Niederacher D; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany., Cheng Z; Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.; Institute of Gynecological Minimally Invasive Medicine, Tongji University School of Medicine, Shanghai, 200072, China., Neubauer H; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany. Hans.Neubauer@med.uni-duesseldorf.de., Stamm N; Department of Obstetrics and Gynecology, Life Science Center, University Hospital and Medical Faculty of the Heinrich-Heine University Duesseldorf, Duesseldorf, Germany. Nadia.Stamm@med.uni-duesseldorf.de.
المصدر: Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2024 Feb 03; Vol. 22 (1), pp. 20. Date of Electronic Publication: 2024 Feb 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101153627 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-7827 (Electronic) Linking ISSN: 14777827 NLM ISO Abbreviation: Reprod Biol Endocrinol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2003-
مواضيع طبية MeSH: Prohibitins* , Progesterone*/pharmacology , Progesterone*/metabolism, Pregnancy ; Female ; Humans ; Decidua/metabolism ; Receptors, Progesterone/genetics ; Progestins/metabolism ; Endometrium/metabolism ; Stromal Cells/metabolism ; Membrane Proteins/genetics ; Membrane Proteins/metabolism
مستخلص: Background: Decidualization of endometrial cells is the prerequisite for embryo implantation and subsequent placenta formation and is induced by rising progesterone levels following ovulation. One of the hormone receptors contributing to endometrial homeostasis is Progesterone Receptor Membrane Component 1 (PGRMC1), a non-classical membrane-bound progesterone receptor with yet unclear function. In this study, we aimed to investigate how PGRMC1 contributes to human decidualization.
Methods: We first analyzed PGRMC1 expression profile during a regular menstrual cycle in RNA-sequencing datasets. To further explore the function of PGRMC1 in human decidualization, we implemented an inducible decidualization system, which is achieved by culturing two human endometrial stromal cell lines in decidualization-inducing medium containing medroxyprogesterone acetate and 8-Br-cAMP. In our system, we measured PGRMC1 expression during hormone induction as well as decidualization status upon PGRMC1 knockdown at different time points. We further conferred proximity ligation assay to identify PGRMC1 interaction partners.
Results: In a regular menstrual cycle, PGRMC1 mRNA expression is gradually decreased from the proliferative phase to the secretory phase. In in vitro experiments, we observed that PGRMC1 expression follows a rise-to-decline pattern, in which its expression level initially increased during the first 6 days after induction (PGRMC1 increasing phase) and decreased in the following days (PGRMC1 decreasing phase). Knockdown of PGRMC1 expression before the induction led to a failed decidualization, while its knockdown after induction did not inhibit decidualization, suggesting that the progestin-induced 'PGRMC1 increasing phase' is essential for normal decidualization. Furthermore, we found that the interactions of prohibitin 1 and prohibitin 2 with PGRMC1 were induced upon progestin treatment. Knocking down each of the prohibitins slowed down the decidualization process compared to the control, suggesting that PGRMC1 cooperates with prohibitins to regulate decidualization.
Conclusions: According to our findings, PGRMC1 expression followed a progestin-induced rise-to-decline expression pattern during human endometrial decidualization process; and the correct execution of this expression program was crucial for successful decidualization. Thereby, the results of our in vitro model explained how PGRMC1 dysregulation during decidualization may present a new perspective on infertility-related diseases.
(© 2024. The Author(s).)
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معلومات مُعتمدة: NE 805/6-1 Deutsche Forschungsgemeinschaft; NE 805/6-1 Deutsche Forschungsgemeinschaft; #51 The Brigitte and Dr. Konstanze Wegener Foundation; #51 The Brigitte and Dr. Konstanze Wegener Foundation; SHDC12021113 The Shanghai Shenkang Hospital Development Center; #48/2018 The Medical Faculty of the Heinrich-Heine University Duesseldorf
فهرسة مساهمة: Keywords: AG205; Decidualization; Endometrium; Infertility; Progesterone receptor membrane component 1 (PGRMC1); Prohibitin-1 (PHB1); Prohibitin-2 (PHB2); Rise-to-decline pattern; Telomerase-immortalized human endometrial stromal cells (T-HESCs)
المشرفين على المادة: 0 (Prohibitins)
4G7DS2Q64Y (Progesterone)
0 (Receptors, Progesterone)
0 (Progestins)
0 (PGRMC1 protein, human)
0 (Membrane Proteins)
تواريخ الأحداث: Date Created: 20240202 Date Completed: 20240205 Latest Revision: 20240205
رمز التحديث: 20240205
مُعرف محوري في PubMed: PMC10837943
DOI: 10.1186/s12958-024-01188-9
PMID: 38308254
قاعدة البيانات: MEDLINE
الوصف
تدمد:1477-7827
DOI:10.1186/s12958-024-01188-9