دورية أكاديمية
Layered double hydroxides (LDHs) as efficient and safe carriers for miRNA inhibitors: In vitro and in vivo assessment of biocompatibility.
| العنوان: | Layered double hydroxides (LDHs) as efficient and safe carriers for miRNA inhibitors: In vitro and in vivo assessment of biocompatibility. |
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| المؤلفون: | de Araújo MA; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil. Electronic address: mykaella.bio@gmail.com., Dos Santos Júnior ED; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Dos Santos BP; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Dos Santos YDR; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Paulino PAT; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Dos Santos EC; Laboratory of Research in Virology and Immunology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Souza TPM; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Anhezini L; Laboratory for in Vivo Analysis of Toxicity and Neurodegenerative Diseases, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Bassi ÊJ; Laboratory of Research in Virology and Immunology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Duzzioni M; Department of Physiology and Pharmacology, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceio, 57072-900, Brazil., de Castro OW; Department of Physiology and Pharmacology, Institute of Biological Sciences and Health, Federal University of Alagoas, Maceio, 57072-900, Brazil., de Andrade TG; Circadian Medicine Center, Faculty of Medicine, Federal University of Alagoas, Maceio, 57072-900, Brazil., Dornelas CB; Laboratory for Active Substance Carrier Nanosystems Technology, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil., Gitaí DLG; Laboratory of Cellular and Molecular Biology, Institute of Biological and Health Sciences, Federal University of Alagoas, Maceio, 57072-900, Brazil. Electronic address: daniel.gitai@icbs.ufal.br. |
| المصدر: | Chemico-biological interactions [Chem Biol Interact] 2024 Mar 01; Vol. 391, pp. 110874. Date of Electronic Publication: 2024 Feb 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Ireland NLM ID: 0227276 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7786 (Electronic) Linking ISSN: 00092797 NLM ISO Abbreviation: Chem Biol Interact Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , Antineoplastic Agents*, Animals ; Drosophila melanogaster ; Hydroxides/chemistry ; Water ; RNA, Small Interfering |
| مستخلص: | Layered double hydroxides (LDHs) have been employed as nano-sized carriers for therapeutic/bio-active molecules, including small interfering RNAs (siRNAs). However, the potential of LDHs nanoparticles for an efficient and safe antisense oligonucleotide (AMO) delivery still requires studies. In this research, we have tested the suitability of a Mg-Al-LDH-based nanocarrier loaded with a miRNA-196b-5p inhibitor. LDHs (and LDH-Oligo complex) were synthesized by the coprecipitation method followed by physicochemical characterization as hydrodynamic size, surface charge, crystallinity, and chemical groups. Thymic endothelial cell line (tEnd.1) were transfected with LDH-Oligo and were evaluated for i. cell viability by MTT, trypan blue, and propidium iodide assays; ii. transfection efficiency by flow cytometry, and iii. depletion of miRNA-196b-5p by RT-qPCR. In addition, Drosophila melanogaster larvae were fed LDHs and evaluated for: i. larval motility; ii. pupation rate; iii. larval-pupal transition; iv. lethality, and v. emergence rate. We demonstrated that LDHs nanoparticles are stable in aqueous solutions and exhibit a regular hexagonal shape. The LDH-AMO complex showed a transfection efficiency of 93.95 ± 2.15 % and induced a significant depletion of miRNA-196b-5p 48h after transfection. No cytotoxic effects were detected in tEnd.1 cells at concentrations up to 50 μg/ml, as well as in Drosophila exposed up to 500 μg of LDH. In conclusion, our data suggest that LDHs are biocompatible and efficient carriers for miRNA inhibitors and can be used as a viable and effective tool in functional miRNA inhibition assays. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Carrier; LDH; Nanotechnology; microRNA |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Hydroxides) 059QF0KO0R (Water) 0 (Antineoplastic Agents) 0 (RNA, Small Interfering) |
| تواريخ الأحداث: | Date Created: 20240204 Date Completed: 20240306 Latest Revision: 20240306 |
| رمز التحديث: | 20240306 |
| DOI: | 10.1016/j.cbi.2024.110874 |
| PMID: | 38311162 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7786 |
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| DOI: | 10.1016/j.cbi.2024.110874 |