دورية أكاديمية
أعرض في EDS

Development of collagenous scaffolds for wound healing: characterization and in vivo analysis.

التفاصيل البيبلوغرافية
العنوان: Development of collagenous scaffolds for wound healing: characterization and in vivo analysis.
المؤلفون: Rodrigues JP; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil. jessica.prt@hotmail.com., da Costa Silva JR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Ferreira BA; Department of Physiological Sciences, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil., Veloso LI; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Quirino LS; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Rosa RR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Barbosa MC; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Rodrigues CM; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Gaspari PBF; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Beletti ME; Department of Cell Biology, Histology and Embryology, Federal University of Uberlândia, UFU, Uberlândia, MG, Brazil., Goulart LR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil., Corrêa NCR; Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of Uberlandia, Av. Amazonas s/n, Campus Umuarama BL-2E, SL-248, Uberlândia, Minas Gerais, 38400-902, Brazil.
المصدر: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2024 Feb 05; Vol. 35 (1), pp. 12. Date of Electronic Publication: 2024 Feb 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 9013087 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4838 (Electronic) Linking ISSN: 09574530 NLM ISO Abbreviation: J Mater Sci Mater Med Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Norwell, MA : Springer
Original Publication: London : Chapman and Hall, c1990-
مواضيع طبية MeSH: Acetylcysteine* , Anti-Infective Agents*/pharmacology , Chitosan*/chemistry, Animals ; Mice ; Biocompatible Materials/chemistry ; Collagen/chemistry ; Tissue Scaffolds/chemistry ; Wound Healing ; Polylysine/chemistry
مستخلص: The development of wound dressings from biomaterials has been the subject of research due to their unique structural and functional characteristics. Proteins from animal origin, such as collagen and chitosan, act as promising materials for applications in injuries and chronic wounds, functioning as a repairing agent. This study aims to evaluate in vitro effects of scaffolds with different formulations containing bioactive compounds such as collagen, chitosan, N-acetylcysteine (NAC) and ε-poly-lysine (ε-PL). We manufactured a scaffold made of a collagen hydrogel bioconjugated with chitosan by crosslinking and addition of NAC and ε-PL. Cell viability was verified by resazurin and live/dead assays and the ultrastructure of biomaterials was evaluated by SEM. Antimicrobial sensitivity was assessed by antibiogram. The healing potential of the biomaterial was evaluated in vivo, in a model of healing of excisional wounds in mice. On the 7th day after the injury, the wounds and surrounding skin were processed for evaluation of biochemical and histological parameters associated with the inflammatory process. The results showed great cell viability and increase in porosity after crosslinking while antimicrobial action was observed in scaffolds containing NAC and ε-PL. Chitosan scaffolds bioconjugated with NAC/ε-PL showed improvement in tissue healing, with reduced lesion size and reduced inflammation. It is concluded that scaffolds crosslinked with chitosan-NAC-ε-PL have the desirable characteristics for tissue repair at low cost and could be considered promising biomaterials in the practice of regenerative medicine.
(© 2024. The Author(s).)
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المشرفين على المادة: WYQ7N0BPYC (Acetylcysteine)
0 (Anti-Infective Agents)
0 (Biocompatible Materials)
9012-76-4 (Chitosan)
0 (chitosan-N-acetyl-L-cysteine)
9007-34-5 (Collagen)
25104-18-1 (Polylysine)
تواريخ الأحداث: Date Created: 20240205 Date Completed: 20240208 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10844142
DOI: 10.1007/s10856-023-06774-8
PMID: 38315254
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4838
DOI:10.1007/s10856-023-06774-8