دورية أكاديمية
Evidence of binding between diethylstilbestrol (DES) and the goldfish ( Carassius auratus ) membrane progesterone receptor α.
| العنوان: | Evidence of binding between diethylstilbestrol (DES) and the goldfish ( Carassius auratus ) membrane progesterone receptor α. |
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| المؤلفون: | Hossain MF; Department of Bioscience, Graduate School of Science and Technology, National University Corporation, Shizuoka University, Shizuoka, Japan., Mustary UH; Department of Bioscience, Graduate School of Science and Technology, National University Corporation, Shizuoka University, Shizuoka, Japan., Tokumoto T; Department of Bioscience, Graduate School of Science and Technology, National University Corporation, Shizuoka University, Shizuoka, Japan. |
| المصدر: | Toxicology mechanisms and methods [Toxicol Mech Methods] 2024 Jun; Vol. 34 (5), pp. 563-571. Date of Electronic Publication: 2024 Feb 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Informa Healthcare Country of Publication: England NLM ID: 101134521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-6524 (Electronic) Linking ISSN: 15376516 NLM ISO Abbreviation: Toxicol Mech Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Informa Healthcare Original Publication: London : Taylor and Francis, c2002- |
| مواضيع طبية MeSH: | Diethylstilbestrol*/toxicity , Fish Proteins*/metabolism , Fish Proteins*/genetics , Goldfish*/metabolism , Receptors, Progesterone*/metabolism, Animals ; Binding, Competitive ; Cell Membrane/metabolism ; Cell Membrane/drug effects ; Oocytes/metabolism ; Oocytes/drug effects ; Progesterone/metabolism ; Protein Binding |
| مستخلص: | Background: In a previous study, diethylstilbestrol (DES) was shown to induce oocyte maturation in fish. In the present study, the interaction of DES on goldfish membrane progesterone receptor α (GmPRα) was investigated using a competitive binding assay with radiolabeled steroids. The results indicate that DES exerts its effects on membrane progesterone receptor alpha (mPRα) and induces oocyte maturation through nongenomic steroid mechanisms. This study provides empirical data that demonstrate the binding between DES and GmPRα. Methods: Binding of DES to GmPRα was achieved by using radiolabeled DES and recombinant GmPRα expressed in culture cells or purified GmPRα proteins that coupled to graphene quantum dots (GQDs). Additionally, the competitive binding of fluorescently labeled progesterone to GmPRα-expressing cells was evaluated. Results: Although significant nonspecific binding of radiolabeled DES to the cell membrane that expresses GmPRα has been observed, specific binding of DES to GmPRα has been successfully identified in the presence of digitonin. Furthermore, the specific binding of DES to GmPRα was confirmed by a binding assay using GQD-GmPRα. The radiolabeled DES was shown to bind to GQD-GmPRα. Additionally, the competition for the binding of fluorescently labeled progesterone to GmPRα-expressing cells was achieved with the DES. Conclusions: The results of the experiments revealed that DES binds to GmPRα. Thus, it can be concluded that DES induces goldfish oocyte maturation by binding to GmPRα. |
| فهرسة مساهمة: | Keywords: Diethylstilbestrol (DES); endocrine disrupting chemicals (EDCs); goldfish; mPRα; oocyte maturation |
| المشرفين على المادة: | 731DCA35BT (Diethylstilbestrol) 0 (Fish Proteins) 4G7DS2Q64Y (Progesterone) 0 (Receptors, Progesterone) |
| تواريخ الأحداث: | Date Created: 20240206 Date Completed: 20240515 Latest Revision: 20240613 |
| رمز التحديث: | 20240613 |
| DOI: | 10.1080/15376516.2024.2311185 |
| PMID: | 38317456 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1537-6524 |
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| DOI: | 10.1080/15376516.2024.2311185 |