دورية أكاديمية
أعرض في EDS

Antigen stasis and airway nitrosative stress in human primary ciliary dyskinesia.

التفاصيل البيبلوغرافية
العنوان: Antigen stasis and airway nitrosative stress in human primary ciliary dyskinesia.
المؤلفون: Gaston B; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States., Smith LA; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States., Davis MD; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States., Saunders J; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States., Daniels I; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States., Horani A; Department of Medicine, Washington University, St. Louis, Missouri, United States., Brody SL; Department of Medicine, Washington University, St. Louis, Missouri, United States., Giddings O; Department of Medicine, Case Western Reserve University, Cleveland, Ohio, United States., Zhao Y; Department of Biostatistics and Health Data Science, Indiana University School of Medicine, Indianapolis, Indiana, United States., Marozkina N; Herman B. Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana, United States.
المصدر: American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2024 Apr 01; Vol. 326 (4), pp. L468-L476. Date of Electronic Publication: 2024 Feb 06.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901229 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1504 (Electronic) Linking ISSN: 10400605 NLM ISO Abbreviation: Am J Physiol Lung Cell Mol Physiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Bethesda, MD : American Physiological Society, c1989-
مواضيع طبية MeSH: Ciliary Motility Disorders* , Kartagener Syndrome*/metabolism, Humans ; Hydrogen Peroxide ; Nitrosative Stress ; Breath Tests ; Nitric Oxide/metabolism ; Biomarkers/metabolism
مستخلص: Nasal nitric oxide (nNO) is low in most patients with primary ciliary dyskinesia (PCD). Decreased ciliary motion could lead to antigen stasis, increasing oxidant production and NO oxidation in the airways. This could both decrease gas phase NO and increase nitrosative stress. We studied primary airway epithelial cells from healthy controls (HCs) and patients with PCD with several different genotypes. We measured antigen clearance in fenestrated membranes exposed apically to the fluorescently labeled antigen Dermatophagoides pteronyssinus (Derp1-f). We immunoblotted for 3-nitrotyrosine (3-NT) and for oxidative response enzymes. We measured headspace NO above primary airway cells without and with a PCD-causing genotype. We measured nNO and exhaled breath condensate (EBC) H 2 O 2 in vivo. Apical Derp1-f was cleared from HC better than from PCD cells. DUOX1 expression was lower in HC than in PCD cells at baseline and after 24-h Derp1-f exposure. HC cells had less 3-NT and NO 3 - than PCD cells. However, NO consumption by HC cells was less than that by PCD cells; NO loss was prevented by superoxide dismutase (SOD) and by apocynin. nNO was higher in HCs than in patients with PCD. EBC H 2 O 2 was lower in HC than in patients with PCD. The PCD airway epithelium does not optimally clear antigens and is subject to oxidative and nitrosative stress. Oxidation associated with antigen stasis could represent a therapeutic target in PCD, one with convenient monitoring biomarkers. NEW & NOTEWORTHY The PCD airway epithelium does not optimally clear antigens, and antigen exposure can lead to NO oxidation and nitrosative stress. Oxidation caused by antigen stasis could represent a therapeutic target in PCD, and there are convenient monitoring biomarkers.
References: Chest. 2016 Jun;149(6):1445-59. (PMID: 26836936)
Sci Rep. 2021 Jul 29;11(1):15498. (PMID: 34326365)
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11393-8. (PMID: 20534503)
Annu Rev Physiol. 2015;77:431-52. (PMID: 25668023)
Free Radic Biol Med. 1994 Jul;17(1):1-10. (PMID: 7959161)
Lancet Respir Med. 2022 May;10(5):459-468. (PMID: 35051411)
Lancet. 2000 Feb 19;355(9204):624. (PMID: 10696986)
Am J Respir Cell Mol Biol. 1995 Apr;12(4):369-78. (PMID: 7695916)
Am J Respir Crit Care Med. 2002 Feb 1;165(3):387-90. (PMID: 11818326)
J Clin Invest. 2002 Feb;109(3):317-25. (PMID: 11827991)
Am J Respir Crit Care Med. 1999 Jul;160(1):1-9. (PMID: 10390372)
J Biol Chem. 2016 Mar 18;291(12):6423-32. (PMID: 26823467)
Am J Respir Crit Care Med. 2004 Dec 15;170(12):1317-23. (PMID: 15466253)
J Allergy Clin Immunol. 2016 May;137(5):1545-1556.e11. (PMID: 26597162)
Transl Sci Rare Dis. 2019;4(1-2):51-75. (PMID: 31572664)
Chron Respir Dis. 2017 Nov;14(4):392-406. (PMID: 29081265)
Redox Biol. 2019 Oct;27:101146. (PMID: 30833143)
Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10089-93. (PMID: 7524082)
Eur Respir J. 2017 Apr 26;49(4):. (PMID: 28446552)
Br J Pharmacol. 2018 May;175(9):1401-1418. (PMID: 29405261)
Pediatr Pulmonol. 2019 Jan;54(1):27-32. (PMID: 30485726)
Methods Mol Biol. 2013;945:109-21. (PMID: 23097104)
J Clin Invest. 1994 Dec;94(6):2407-13. (PMID: 7989597)
Am J Respir Crit Care Med. 2019 Jan 15;199(2):190-198. (PMID: 30067075)
J Immunol. 2008 May 15;180(10):6911-22. (PMID: 18453612)
J Biol Chem. 2001 Oct 26;276(43):39872-8. (PMID: 11518706)
Nat Genet. 2014 Jun;46(6):646-51. (PMID: 24747639)
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 15;851(1-2):140-51. (PMID: 17379583)
BMC Med Genet. 2020 Nov 26;21(1):237. (PMID: 33243178)
J Biol Chem. 2016 Oct 28;291(44):23282-23293. (PMID: 27650496)
Am J Respir Crit Care Med. 2023 Jun 1;207(11):1486-1497. (PMID: 36952660)
Am J Physiol Lung Cell Mol Physiol. 2019 Aug 1;317(2):L247-L258. (PMID: 31116581)
Respir Med. 2013 Mar;107(3):380-6. (PMID: 23290188)
Am J Respir Crit Care Med. 2004 Feb 15;169(4):459-67. (PMID: 14656747)
Mediators Inflamm. 2008;2008:106507. (PMID: 19096513)
Pediatr Pulmonol. 2021 Aug;56(8):2776-2779. (PMID: 34102041)
Am J Pathol. 2005 Mar;166(3):663-74. (PMID: 15743779)
Respir Med. 2008 Apr;102(4):541-7. (PMID: 18187313)
PLoS One. 2014 Oct 17;9(10):e110708. (PMID: 25329652)
J Clin Med. 2022 Nov 16;11(22):. (PMID: 36431268)
Pediatr Pulmonol. 2016 Feb;51(2):115-32. (PMID: 26418604)
J Clin Invest. 1999 Jul;104(1):123-33. (PMID: 10393706)
J Pediatr. 2011 Aug;159(2):248-55.e1. (PMID: 21392781)
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2622-7. (PMID: 11226289)
معلومات مُعتمدة: P01 HL128192 United States HL NHLBI NIH HHS; P01 HL158507 United States HL NHLBI NIH HHS; R01 HL128370 United States HL NHLBI NIH HHS; R01 HL146601 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: DUOX1; nasal NO; nitrosative stress; oxidation; primary ciliary dyskinesia
المشرفين على المادة: BBX060AN9V (Hydrogen Peroxide)
31C4KY9ESH (Nitric Oxide)
0 (Biomarkers)
تواريخ الأحداث: Date Created: 20240206 Date Completed: 20240328 Latest Revision: 20240728
رمز التحديث: 20240728
مُعرف محوري في PubMed: PMC11281798
DOI: 10.1152/ajplung.00208.2022
PMID: 38318660
قاعدة البيانات: MEDLINE
الوصف
تدمد:1522-1504
DOI:10.1152/ajplung.00208.2022