دورية أكاديمية
Antibacterial synergy between a phage endolysin and citric acid against the Gram-negative kiwifruit pathogen Pseudomonas syringae pv. actinidiae .
| العنوان: | Antibacterial synergy between a phage endolysin and citric acid against the Gram-negative kiwifruit pathogen Pseudomonas syringae pv. actinidiae . |
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| المؤلفون: | Sisson HM; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Bioprotection Aotearoa, University of Otago, Dunedin, New Zealand., Fagerlund RD; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Bioprotection Aotearoa, University of Otago, Dunedin, New Zealand.; Genetics Otago, University of Otago, Dunedin, New Zealand.; Maurice Wilkins Centre for Molecular Biodiscovery, University of Otago, Dunedin, New Zealand., Jackson SA; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Bioprotection Aotearoa, University of Otago, Dunedin, New Zealand.; Genetics Otago, University of Otago, Dunedin, New Zealand.; Maurice Wilkins Centre for Molecular Biodiscovery, University of Otago, Dunedin, New Zealand., Briers Y; Laboratory of Applied Biotechnology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium., Warring SL; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand., Fineran PC; Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.; Bioprotection Aotearoa, University of Otago, Dunedin, New Zealand.; Genetics Otago, University of Otago, Dunedin, New Zealand.; Maurice Wilkins Centre for Molecular Biodiscovery, University of Otago, Dunedin, New Zealand. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2024 Mar 20; Vol. 90 (3), pp. e0184623. Date of Electronic Publication: 2024 Feb 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Bacteriophages* , Actinidia*/microbiology , Endopeptidases*, Pseudomonas syringae ; Copper ; Peptidoglycan ; Plant Diseases/prevention & control ; Plant Diseases/microbiology ; Anti-Bacterial Agents/pharmacology |
| مستخلص: | Horticultural diseases caused by bacterial pathogens provide an obstacle to crop production globally. Management of the infection of kiwifruit by the Gram-negative phytopathogen Pseudomonas syringae pv. actinidiae ( Psa ) currently includes copper and antibiotics. However, the emergence of bacterial resistance and a changing regulatory landscape are providing the impetus to develop environmentally sustainable antimicrobials. One potential strategy is the use of bacteriophage endolysins, which degrade peptidoglycan during normal phage replication, causing cell lysis and the release of new viral progeny. Exogenous use of endolysins as antimicrobials is impaired by the outer membrane of Gram-negative bacteria that provides an impermeable barrier and prevents endolysins from accessing their target peptidoglycan. Here, we describe the synergy between citric acid and a phage endolysin, which results in a reduction of viable Psa below detection. We show that citric acid drives the destabilization of the outer membrane via acidification and sequestration of divalent cations from the lipopolysaccharide, which is followed by the degradation of the peptidoglycan by the endolysin. Scanning electron microscopy revealed clear morphological differences, indicating cell lysis following the endolysin-citric acid treatment. These results show the potential for citric acid-endolysin combinations as a possible antimicrobial approach in agricultural applications. Importance: The phytopathogen Pseudomonas syringae pv. actinidiae ( Psa ) causes major impacts to kiwifruit horticulture, and the current control strategies are heavily reliant on copper and antibiotics. The environmental impact and increasing resistance to these agrichemicals are driving interest in alternative antimicrobials including bacteriophage-derived therapies. In this study, we characterize the endolysin from the Otagovirus Psa374 which infects Psa . When combined with citric acid, this endolysin displays an impressive antibacterial synergy to reduce viable Psa below the limit of detection. The use of citric acid as a synergistic agent with endolysins has not been extensively studied and has never been evaluated against a plant pathogen. We determined that the synergy involved a combination of the chelation activity of citric acid, acidic pH, and the specific activity of the ΦPsa374 endolysin. Our study highlights an exciting opportunity for alternative antimicrobials in agriculture. Competing Interests: The authors declare no conflict of interest. |
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| معلومات مُعتمدة: | Ministry for Business Innovation and Employment (MBIE) |
| فهرسة مساهمة: | Keywords: Pseudomonas syringae; bacteriophages; biocontrol; chelation; citric acid; endolysin |
| المشرفين على المادة: | EC 3.4.99.- (endolysin) 789U1901C5 (Copper) 0 (Peptidoglycan) 0 (Anti-Bacterial Agents) EC 3.4.- (Endopeptidases) |
| تواريخ الأحداث: | Date Created: 20240206 Date Completed: 20240321 Latest Revision: 20240807 |
| رمز التحديث: | 20240807 |
| مُعرف محوري في PubMed: | PMC10952447 |
| DOI: | 10.1128/aem.01846-23 |
| PMID: | 38319087 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
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| DOI: | 10.1128/aem.01846-23 |