دورية أكاديمية
أعرض في EDS

Successful xenotransplantation of testicular cells following fractionated chemotherapy of recipient birds.

التفاصيل البيبلوغرافية
العنوان: Successful xenotransplantation of testicular cells following fractionated chemotherapy of recipient birds.
المؤلفون: Blank MH; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Duque de Caxias Norte 255, Pirassununga, SP , CEP 13635-900, Brazil. marcel.henriqueblank@gmail.com., Kawaoku AJT; Hendrix Genetics Brazil, Estrada Municipal Slt-161 Km 08, Salto, 13328-400, Brazil., Rui BR; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Duque de Caxias Norte 255, Pirassununga, SP , CEP 13635-900, Brazil., Carreira ACO; Cell and Molecular Therapy Center (NUCEL), Medical School, University of Sao Paulo, Rua Pangaré 100, São Paulo, 05360-130, Brazil., Hamilton TRDS; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Duque de Caxias Norte 255, Pirassununga, SP , CEP 13635-900, Brazil., Goissis MD; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Duque de Caxias Norte 255, Pirassununga, SP , CEP 13635-900, Brazil., Pereira RJG; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, Av. Duque de Caxias Norte 255, Pirassununga, SP , CEP 13635-900, Brazil. ricpereira@usp.br.
المصدر: Scientific reports [Sci Rep] 2024 Feb 07; Vol. 14 (1), pp. 3085. Date of Electronic Publication: 2024 Feb 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Testis* , Spermatogonia*, Male ; Animals ; Busulfan ; Chickens ; Transplantation, Heterologous ; Semen ; Spermatogenesis ; Quail
مستخلص: An essential step in the success of germ cell transplantation is the preparation of the recipient's testicular environment to increase the availability of stem cell niches. However, most methods for this purpose in birds face serious limitations such as partial germ cell depletion, high toxicity and mortality, or the need to use expensive technologies. Here, we validated a simple and practical technique of transferring quail testicular cells into chicken testes depleted of endogenous spermatozoa by fractioned chemotherapy (20 mg/kg/week busulfan for 5 weeks). This protocol resulted in a very low mortality of the treated day-old chicks and, despite maintenance of androgenic activity, sperm production was decreased by 84.3% at 25 weeks of age. NANOG immunostaining revealed that very few to no germ cells were present following treatment with 20 and 40 mg/kg, respectively. RT-qPCR data also showed that c-MYC and NANOG expression declined in these treatments, but GRFα1 and BID expressions remained unaltered among groups. After xenotransplantation, quail germ cells were immunodetected in chicken testes using a species-specific antibody (QCPN), and quail ovalbumin DNA was found in seminal samples collected from chicken recipients. Together, these data confirm that fractionated administration of busulfan in hatchlings is a practical, effective, and safe protocol to prepare recipient male birds capable of supporting xenogeneic spermatogenesis.
(© 2024. The Author(s).)
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معلومات مُعتمدة: 2020/11479-9 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2013/21839-3 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2012/ 08621-1 Fundação de Amparo à Pesquisa do Estado de São Paulo
المشرفين على المادة: G1LN9045DK (Busulfan)
تواريخ الأحداث: Date Created: 20240206 Date Completed: 20240208 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10847125
DOI: 10.1038/s41598-023-45019-0
PMID: 38321093
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-45019-0