دورية أكاديمية
Spatial MS multiomics on clinical prostate cancer tissues.
| العنوان: | Spatial MS multiomics on clinical prostate cancer tissues. |
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| المؤلفون: | Truong JXM; The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia.; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia.; Freemasons Centre for Male Health and Wellbeing, University of Adelaide, North Terrace, Adelaide, South Australia, 5000, Australia.; South Australian immunoGENomics Cancer Institute (SAiGENCI), North Terrace, Adelaide, South Australia, 5000, Australia., Rao SR; The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia.; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia., Ryan FJ; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia.; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, South Australia, 5042, Australia., Lynn DJ; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia.; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, South Australia, 5042, Australia., Snel MF; The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia.; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia., Butler LM; The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia.; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia.; Freemasons Centre for Male Health and Wellbeing, University of Adelaide, North Terrace, Adelaide, South Australia, 5000, Australia.; South Australian immunoGENomics Cancer Institute (SAiGENCI), North Terrace, Adelaide, South Australia, 5000, Australia., Trim PJ; The University of Adelaide, North Terrace, Adelaide, SA, 5000, Australia. Paul.Trim@sahmri.com.; South Australian Health and Medical Research Institute (SAHMRI), North Terrace, Adelaide, South Australia, 5000, Australia. Paul.Trim@sahmri.com. |
| المصدر: | Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Mar; Vol. 416 (7), pp. 1745-1757. Date of Electronic Publication: 2024 Feb 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Heidelberg : Springer-Verlag, 2002- |
| مواضيع طبية MeSH: | Multiomics* , Prostatic Neoplasms*, Male ; Humans ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Laser Capture Microdissection ; Phosphatidylcholines/metabolism |
| مستخلص: | Mass spectrometry (MS) and MS imaging (MSI) are used extensively for both the spatial and bulk characterization of samples in lipidomics and proteomics workflows. These datasets are typically generated independently due to different requirements for sample preparation. However, modern omics technologies now provide higher sample throughput and deeper molecular coverage, which, in combination with more sophisticated bioinformatic and statistical pipelines, make generating multiomics data from a single sample a reality. In this workflow, we use spatial lipidomics data generated by matrix-assisted laser desorption/ionization MSI (MALDI-MSI) on prostate cancer (PCa) radical prostatectomy cores to guide the definition of tumor and benign tissue regions for laser capture microdissection (LCM) and bottom-up proteomics all on the same sample and using the same mass spectrometer. Accurate region of interest (ROI) mapping was facilitated by the SCiLS region mapper software and dissected regions were analyzed using a dia-PASEF workflow. A total of 5525 unique protein groups were identified from all dissected regions. Lysophosphatidylcholine acyltransferase 1 (LPCAT1), a lipid remodelling enzyme, was significantly enriched in the dissected regions of cancerous epithelium (CE) compared to benign epithelium (BE). The increased abundance of this protein was reflected in the lipidomics data with an increased ion intensity ratio for pairs of phosphatidylcholines (PC) and lysophosphatidylcholines (LPC) in CE compared to BE. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Laser capture microdissection; Lipidomics; MALDI imaging; Multiomics; Prostate cancer; Proteomics |
| المشرفين على المادة: | 0 (Phosphatidylcholines) |
| تواريخ الأحداث: | Date Created: 20240207 Date Completed: 20240229 Latest Revision: 20240313 |
| رمز التحديث: | 20240313 |
| DOI: | 10.1007/s00216-024-05178-z |
| PMID: | 38324070 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1618-2650 |
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| DOI: | 10.1007/s00216-024-05178-z |