دورية أكاديمية
أعرض في EDS

A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities.

التفاصيل البيبلوغرافية
العنوان: A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities.
المؤلفون: Peng H; Department of Bioengineering, Imperial College London, London, UK.; Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Darlington APS; Warwick Integrative Synthetic Biology Centre, School of Engineering, University of Warwick, Coventry, UK., South EJ; Molecular Biology, Cell Biology and Biochemistry Program, Boston University, Boston, MA, USA., Chen HH; Department of Bioengineering, Imperial College London, London, UK.; Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.; School of Food Science and Engineering, South China University of Technology, Guangzhou, China., Jiang W; Department of Bioengineering, Imperial College London, London, UK.; Imperial College Centre for Synthetic Biology, Imperial College London, London, UK.; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Ledesma-Amaro R; Department of Bioengineering, Imperial College London, London, UK. r.ledesma-amaro@imperial.ac.uk.; Imperial College Centre for Synthetic Biology, Imperial College London, London, UK. r.ledesma-amaro@imperial.ac.uk.
المصدر: Nature microbiology [Nat Microbiol] 2024 Mar; Vol. 9 (3), pp. 848-863. Date of Electronic Publication: 2024 Feb 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101674869 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2058-5276 (Electronic) Linking ISSN: 20585276 NLM ISO Abbreviation: Nat Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Publishing Group, [2016]-
مواضيع طبية MeSH: Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Metabolic Engineering*, Microbial Consortia/genetics ; Synthetic Biology ; Engineering
مستخلص: Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model and key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic and overexpression yeast strains that can be used to create co-cultures through exchange of essential metabolites. Using these strains as modules, we engineered two- and three-member consortia using different cross-feeding architectures. Through a combination of ensemble modelling and experimentation, we explored how cellular (for example, metabolite production strength) and environmental (for example, initial population ratio, population density and extracellular supplementation) factors govern population dynamics in these systems. We tested the use of the toolkit in a division of labour biomanufacturing case study and show that it enables enhanced and tuneable antioxidant resveratrol production. We expect this toolkit to become a useful resource for a variety of applications in synthetic ecology and biomanufacturing.
(© 2024. The Author(s).)
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معلومات مُعتمدة: BB/R01602X/1, BB/T013176/1, BB/T011408/1 RCUK | Biotechnology and Biological Sciences Research Council (BBSRC); DEUSBIO - 949080, PERFECOAT- 101022370, Skindev -101098826 European Commission (EC)
تواريخ الأحداث: Date Created: 20240207 Date Completed: 20240307 Latest Revision: 20240308
رمز التحديث: 20240308
مُعرف محوري في PubMed: PMC10914607
DOI: 10.1038/s41564-023-01596-4
PMID: 38326570
قاعدة البيانات: MEDLINE
الوصف
تدمد:2058-5276
DOI:10.1038/s41564-023-01596-4