دورية أكاديمية
Effects of non-nutritive sweeteners on growth and intestinal health by regulating hypothalamic RNA profile and ileum microbiota in guinea pigs.
| العنوان: | Effects of non-nutritive sweeteners on growth and intestinal health by regulating hypothalamic RNA profile and ileum microbiota in guinea pigs. |
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| المؤلفون: | Zhu S; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.; College of Agriculture, Jinhua Polytechnic, Jinhua, China., Li J; College of Agriculture, Jinhua Polytechnic, Jinhua, China., Li Z; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Wang Z; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Wei Q; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Shi F; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. |
| المصدر: | Journal of the science of food and agriculture [J Sci Food Agric] 2024 May; Vol. 104 (7), pp. 4342-4353. Date of Electronic Publication: 2024 Feb 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: England NLM ID: 0376334 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0010 (Electronic) Linking ISSN: 00225142 NLM ISO Abbreviation: J Sci Food Agric Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005-> : Chichester, West Sussex : John Wiley & Sons Original Publication: London, Society of Chemical Industry. |
| مواضيع طبية MeSH: | Non-Nutritive Sweeteners* , Gastrointestinal Microbiome*, Guinea Pigs ; Animals ; Body Weight ; Ileum ; RNA, Messenger |
| مستخلص: | Background: Non-nutritive sweeteners (NNS) are commonly used in sweetened foods and beverages; however their role in metabolic regulation is still not clear. In this experiment, we used guinea pigs as an animal model to study the effect of NNS on body growth and intestinal health by modifying gut microbiota and hypothalamus-related proteins. Results: For a 28-day feeding experiment a total of 40 guinea pigs were randomly divided into four groups, one control (CN) group and three treatments, in which three NNS were added to the diet: rebaudioside A (RA, 330 mg kg -1 ), sodium saccharin (SS, 800 mg kg -1 ), and sucralose (TGS, 167 mg kg -1 ), respectively. The TGS group exhibited significantly reduced food consumption in comparison with the CN group (P < 0.05) whereas the RA group showed increased food consumption in comparison with the CN group (P < 0.05). Notably, Taste receptor type 1 subunit 2 (T1R2) expression in the hypothalamus was significantly higher in the RA group than in the CN group (P < 0.05). The mRNA expressions of appetite-stimulated genes arouti-related neuropeptide (AGRP), neuropeptide Y (NPY), and thyroid stimulating hormone (TSHB) were significantly higher than those in the CN group (P < 0.05) but mRNA expressions of appetite-suppressed genes tryptophan hydroxylase 2(THP2) were significantly lower in the TGS group (P < 0.05). Furthermore, NNS in the guinea pig diets (RA, SS, TGS) significantly increased the relative abundance of Muribaculaceae but decreased the relative abundance of Clostridia_vadin BB60 in comparison with the CN group (P < 0.05). We also found that dietary supplementation with RA also significantly altered the relative abundance of Lactobacillus. Conclusion: Our finding confirmed that dietary supplementation with RA and TGS affected body growth and intestinal health by modulating hypothalamic RNA profiles and ileum microbiota, suggesting that NNS should be included in guinea-pig feeding. © 2024 Society of Chemical Industry. (© 2024 Society of Chemical Industry.) |
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| معلومات مُعتمدة: | 31972565 National Natural Science Foundation of China; KYT2023002 Fundamental Research Funds for the Central Universities |
| فهرسة مساهمة: | Keywords: guinea pig; gut microbiota; hypothalamus; non‐nutritive sweetener |
| المشرفين على المادة: | 0 (Non-Nutritive Sweeteners) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20240208 Date Completed: 20240411 Latest Revision: 20240411 |
| رمز التحديث: | 20240411 |
| DOI: | 10.1002/jsfa.13320 |
| PMID: | 38328855 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1097-0010 |
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| DOI: | 10.1002/jsfa.13320 |