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Results of an international interlaboratory study on dioxin-like activities in drinking-, river surface- and wastewater using DR CALUX bioassay.

التفاصيل البيبلوغرافية
العنوان: Results of an international interlaboratory study on dioxin-like activities in drinking-, river surface- and wastewater using DR CALUX bioassay.
المؤلفون: Behnisch P; BioDetection Systems B.V. (BDS), Science Park 406, 1098 XH Amsterdam, the Netherlands. Electronic address: Behnisch@bds.nl., Besselink H; BioDetection Systems B.V. (BDS), Science Park 406, 1098 XH Amsterdam, the Netherlands., Suzuki G; Center for Material Cycles and Waste Management Research, National Institute for Environmental Studies (NIES), Tsukuba 305-8506, Japan., Buchinger S; Bundesanstalt für Gewässerkunde (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany., Reifferscheid G; Bundesanstalt für Gewässerkunde (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany., Lukas M; Wastewater Analysis, Monitoring Methods, German Environment Agency (UBA), Berlin, Germany., Wollenweber M; Goethe University Frankfurt/Main (GU), Department Evolutionary Ecology and Environmental Toxicology, Max-von-Laue-Strasse 13, 60438 Frankfurt/Main, Germany., Wollenweber S; Goethe University Frankfurt/Main (GU), Department Evolutionary Ecology and Environmental Toxicology, Max-von-Laue-Strasse 13, 60438 Frankfurt/Main, Germany., Hollert H; Goethe University Frankfurt/Main (GU), Department Evolutionary Ecology and Environmental Toxicology, Max-von-Laue-Strasse 13, 60438 Frankfurt/Main, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Department Environmental Media Related Ecotoxicology, Auf dem Aberg 1, 57392 Schmallenberg, Germany., Kunisue T; Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan., Tue NM; Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, Matsuyama 790-8577, Japan., Alijagic A; Man-Technology-Environment Research Centre (MTM), School of Science and Technology, Örebro University (ORU), SE-701 82 Örebro, Sweden., Larsson M; Man-Technology-Environment Research Centre (MTM), School of Science and Technology, Örebro University (ORU), SE-701 82 Örebro, Sweden., Engwall M; Man-Technology-Environment Research Centre (MTM), School of Science and Technology, Örebro University (ORU), SE-701 82 Örebro, Sweden., Ohno K; Center of International Cooperation and Environmental Technologies of the Japan Environmental Management Association for Industry (JEMAI), 3-1, Uchisaiwaicho 1-chome Chiyoda-ku, Tokyo, Japan., Brouwer A; BioDetection Systems B.V. (BDS), Science Park 406, 1098 XH Amsterdam, the Netherlands; VU University Amsterdam (VU), Faculty of Sciences, Department of Animal Ecology, De Boelelaan 1085, 1081 HV Amsterdam, the Netherlands. Electronic address: bram.brouwer@bds.nl.
المصدر: The Science of the total environment [Sci Total Environ] 2024 Apr 10; Vol. 920, pp. 170759. Date of Electronic Publication: 2024 Feb 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier.
مواضيع طبية MeSH: Polychlorinated Dibenzodioxins*/analysis , Dioxins*/toxicity , Dioxins*/analysis , Polychlorinated Biphenyls*/analysis, Rats ; Animals ; Wastewater ; Reproducibility of Results ; Dibenzofurans/analysis ; Rivers ; Luciferases ; Biological Assay/methods ; Dibenzofurans, Polychlorinated/analysis
مستخلص: Aquatic animals and consumers of aquatic animals are exposed to increasingly complex mixtures of known and as-yet-unknown chemicals with dioxin-like toxicities in the water cycle. Effect- and cell-based bioanalysis can cover known and yet unknown dioxin and dioxin-like compounds as well as complex mixtures thereof but need to be standardized and integrated into international guidelines for environmental testing. In an international laboratory testing (ILT) following ISO/CD 24295 as standard procedure for rat cell-based DR CALUX un-spiked and spiked extracts of drinking-, surface-, and wastewater were validated to generate precision data for the development of the full ISO-standard. We found acceptable repeatability and reproducibility ranges below 36 % by DR CALUX bioassay for the tested un-spiked and spiked water of different origins. The presence of 17 PCDD/Fs and 12 dioxin-like PCBs was also confirmed by congener-specific GC-HRMS analysis. We compared the sum of dioxin-like activity levels measured by DR CALUX bioassay (expressed in 2,3,7,8-TCDD Bioanalytical Equivalents, BEQ; ISO 23196, 2022) with the obtained GC-HRMS chemical analysis results converted to toxic equivalents (TEQ; van den Berg et al., 2013).
Competing Interests: Declaration of competing interest The authors are not involved in water production, recycling of water, or any other activity related to the product water, except for the analyses of contaminants. Therefore, we declare no conflict of interest. Peter Behnisch, Harrie Besselink and Abraham Brouwer are employee of BioDetection Systems. All stable transfected rat cell-lines were provided by the company.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: DR CALUX; Dioxins and dioxin-like compounds; Effect-based method (EBM); International laboratory trial (ILT); Polychlorinated biphenyls (PCBs); Polychlorinated dibenzo-p-dioxin and dibenzofurans (PCDD/PCDFs)
المشرفين على المادة: 0 (Polychlorinated Dibenzodioxins)
0 (Dioxins)
0 (Wastewater)
0 (Dibenzofurans)
EC 1.13.12.- (Luciferases)
DFC2HB4I0K (Polychlorinated Biphenyls)
0 (Dibenzofurans, Polychlorinated)
تواريخ الأحداث: Date Created: 20240209 Date Completed: 20240306 Latest Revision: 20240306
رمز التحديث: 20240306
DOI: 10.1016/j.scitotenv.2024.170759
PMID: 38336065
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1026
DOI:10.1016/j.scitotenv.2024.170759