دورية أكاديمية
أعرض في EDS

Identification and Validation of Magnolol Biosynthesis Genes in Magnolia officinalis .

التفاصيل البيبلوغرافية
العنوان: Identification and Validation of Magnolol Biosynthesis Genes in Magnolia officinalis .
المؤلفون: Yang Y; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China., Li Z; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China., Zong H; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China., Liu S; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Du Q; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Wu H; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Li Z; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China., Wang X; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Huang L; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Lai C; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China., Zhang M; Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, China., Wang W; School of Ecology and Environment, Northwestern Polytechnical University, Xi'an 710072, China., Chen X; Jiaxing Synbiolab Biotechnology Co., Ltd., Jiaxing 314006, China.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2024 Jan 25; Vol. 29 (3). Date of Electronic Publication: 2024 Jan 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Magnolia*/genetics , Magnolia*/chemistry , Lignans*/chemistry , Allyl Compounds* , Phenols*, Humans ; Laccase ; Biphenyl Compounds/chemistry
مستخلص: Bacterial infections pose a significant risk to human health. Magnolol, derived from Magnolia officinalis , exhibits potent antibacterial properties. Synthetic biology offers a promising approach to manufacture such natural compounds. However, the plant-based biosynthesis of magnolol remains obscure, and the lack of identification of critical genes hampers its synthetic production. In this study, we have proposed a one-step conversion of magnolol from chavicol using laccase. After leveraging 20 transcriptomes from diverse parts of M. officinalis , transcripts were assembled, enriching genome annotation. Upon integrating this dataset with current genomic information, we could identify 30 laccase enzymes. From two potential gene clusters associated with magnolol production, highly expressed genes were subjected to functional analysis. In vitro experiments confirmed MoLAC14 as a pivotal enzyme in magnolol synthesis. Improvements in the thermal stability of MoLAC14 were achieved through selective mutations, where E345P, G377P, H347F, E346C, and E346F notably enhanced stability. By conducting alanine scanning, the essential residues in MoLAC14 were identified, and the L532A mutation further boosted magnolol production to an unprecedented level of 148.83 mg/L. Our findings not only elucidated the key enzymes for chavicol to magnolol conversion, but also laid the groundwork for synthetic biology-driven magnolol production, thereby providing valuable insights into M. officinalis biology and comparative plant science.
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فهرسة مساهمة: Keywords: Magnolia officinalis; enzyme activity; in vitro; magnolol synthesis; transcripts
المشرفين على المادة: 001E35HGVF (magnolol)
0 (4-allylphenol)
EC 1.10.3.2 (Laccase)
0 (Lignans)
0 (Biphenyl Compounds)
0 (Allyl Compounds)
0 (Phenols)
تواريخ الأحداث: Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214
رمز التحديث: 20240214
مُعرف محوري في PubMed: PMC10856379
DOI: 10.3390/molecules29030587
PMID: 38338333
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules29030587