دورية أكاديمية
أعرض في EDS

New Insights into Pediatric Kidney Transplant Rejection Biomarkers: Tissue, Plasma and Urine MicroRNAs Compared to Protocol Biopsy Histology.

التفاصيل البيبلوغرافية
العنوان: New Insights into Pediatric Kidney Transplant Rejection Biomarkers: Tissue, Plasma and Urine MicroRNAs Compared to Protocol Biopsy Histology.
المؤلفون: Carraro A; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy., De Gaspari P; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy.; Laboratory Reference, Euroimmun Italy, 35127 Padua, Italy., Antoniello B; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy., Marzenta D; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy.; Pediatric Nephrology, Department of Women's and Children's Health, Padua University Hospital, 35128 Padua, Italy., Vianello E; Pediatric Nephrology, Department of Women's and Children's Health, Padua University Hospital, 35128 Padua, Italy., Bussolati B; Department of Molecular Biotechnology and Health Sciences, University of Turin, 10124 Torino, Italy., Tritta S; Department of Molecular Biotechnology and Health Sciences, University of Turin, 10124 Torino, Italy., Collino F; Department of Clinical Sciences and Community Health, University of Milano, 20126 Milan, Italy.; Paediatric Nephrology, Dialysis and Transplant Unit, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy., Bertoldi L; BMR Genomics srl, 35131 Padua, Italy., Benvenuto G; BMR Genomics srl, 35131 Padua, Italy., Vedovelli L; Unit of Biostatistics, Epidemiology and Public Health, Department of Cardio-Thoraco-Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy., Benetti E; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy.; Pediatric Nephrology, Department of Women's and Children's Health, Padua University Hospital, 35128 Padua, Italy.; Pediatric Research Institute 'IRP Città della Speranza', 35127 Padua, Italy., Negrisolo S; Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Women's and Children's Health, University of Padova, 35127 Padua, Italy.; Pediatric Research Institute 'IRP Città della Speranza', 35127 Padua, Italy.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2024 Feb 05; Vol. 25 (3). Date of Electronic Publication: 2024 Feb 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: MicroRNAs*/genetics , Kidney Transplantation*/adverse effects, Humans ; Child ; Retrospective Studies ; Kidney/pathology ; Biopsy ; Biomarkers/urine ; Graft Rejection/pathology
مستخلص: The early identification of a subclinical rejection (SCR) can improve the long-term outcome of the transplanted kidney through intensified immunosuppression. However, the only approved diagnostic method is the protocol biopsy, which remains an invasive method and not without minor and/or major complications. The protocol biopsy is defined as the sampling of allograft tissue at pre-established times even in the absence of an impaired renal function; however, it does not avoid histological damage. Therefore, the discovery of new possible biomarkers useful in the prevention of SCR has gained great interest. Among all the possible candidates, there are microRNAs (miRNAs), which are short, noncoding RNA sequences, that are involved in mediating numerous post-transcriptional pathways. They can be found not only in tissues, but also in different biological fluids, both as free particles and contained in extracellular vesicles (EVs) released by different cell types. In this study, we firstly performed a retrospective miRNA screening analysis on biopsies and serum EV samples of 20 pediatric transplanted patients, followed by a second screening on another 10 pediatric transplanted patients' urine samples at one year post-transplant. In both cohorts, we divided the patients into two groups: patients with histological SCR and patients without histological SCR at one year post-transplantation. The isolated miRNAs were analyzed in an NGS platform to identify different expressions in the two allograft states. Although no statistical data were found in sera, in the tissue and urinary EVs, we highlighted signatures of miRNAs associated with the histological SCR state.
References: Am J Transplant. 2006 Sep;6(9):2006-12. (PMID: 16796717)
Kidney Int. 2020 Aug;98(2):436-447. (PMID: 32624181)
PLoS One. 2015 Jun 16;10(6):e0127175. (PMID: 26079930)
Ann Oncol. 2021 Apr;32(4):466-477. (PMID: 33548389)
Kidney Int Suppl (2011). 2011 Aug;1(2):36-39. (PMID: 25018901)
Anal Bioanal Chem. 2021 Oct;413(24):5995-6011. (PMID: 34363087)
J Transl Med. 2021 Aug 13;19(1):346. (PMID: 34389032)
Pediatr Nephrol. 2023 Sep;38(9):2939-2955. (PMID: 36648536)
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5330-5. (PMID: 19289845)
Pediatr Clin North Am. 2019 Feb;66(1):269-280. (PMID: 30454748)
Transpl Immunol. 2015 Sep;33(1):1-6. (PMID: 26002284)
Br J Clin Pharmacol. 2017 Dec;83(12):2636-2650. (PMID: 28880456)
Transplant Proc. 2019 Apr;51(3):715-721. (PMID: 30979455)
Lab Invest. 2020 Nov;100(11):1436-1446. (PMID: 32514126)
PLoS One. 2013;8(4):e60702. (PMID: 23577151)
Mol Cancer. 2022 Nov 1;21(1):207. (PMID: 36320056)
Front Immunol. 2023 Aug 22;14:1206929. (PMID: 37675106)
Biomolecules. 2021 Aug 12;11(8):. (PMID: 34439863)
Nat Rev Nephrol. 2017 Dec;13(12):731-749. (PMID: 29081510)
Semin Intervent Radiol. 2019 Jun;36(2):97-103. (PMID: 31123379)
Pediatr Nephrol. 2023 Sep 29;:. (PMID: 37775581)
Transplantation. 2010 Jul 15;90(1):68-74. (PMID: 20463643)
Front Immunol. 2023 Aug 17;14:1154650. (PMID: 37662919)
N Engl J Med. 2004 Jun 24;350(26):2654-62. (PMID: 15215481)
Int J Mol Sci. 2021 Sep 28;22(19):. (PMID: 34638835)
Nat Rev Nephrol. 2015 Jan;11(1):23-33. (PMID: 25385286)
Nat Rev Nephrol. 2022 Oct;18(10):643-662. (PMID: 35974169)
Transplant Proc. 2015 Jul-Aug;47(6):1683-7. (PMID: 26293033)
Front Immunol. 2023 May 09;14:1190576. (PMID: 37228607)
J Nanobiotechnology. 2021 Aug 21;19(1):250. (PMID: 34419056)
J Extracell Vesicles. 2018 Nov 23;7(1):1535750. (PMID: 30637094)
Nat Rev Mol Cell Biol. 2014 Aug;15(8):509-24. (PMID: 25027649)
Front Pediatr. 2023 Jan 20;10:1087841. (PMID: 36741087)
Transplantation. 2021 Jun 1;105(6):1176-1187. (PMID: 33196628)
PLoS One. 2016 Jan 06;11(1):e0146378. (PMID: 26734715)
PLoS One. 2021 Jan 22;16(1):e0245880. (PMID: 33481955)
Int J Mol Med. 2021 Sep;48(3):. (PMID: 34278441)
N Engl J Med. 2014 Aug 7;371(6):549-58. (PMID: 25099579)
Transplantation. 2018 Nov;102(11):1795-1814. (PMID: 30028786)
Clin Lab Med. 2019 Mar;39(1):125-143. (PMID: 30709501)
J Transl Med. 2011 Jun 08;9:86. (PMID: 21651777)
Cell Physiol Biochem. 2019;52(6):1463-1483. (PMID: 31099507)
Sci Rep. 2018 Jul 27;8(1):11329. (PMID: 30054510)
J Extracell Vesicles. 2022 May;11(5):e12213. (PMID: 35524458)
Int J Mol Sci. 2022 Oct 14;23(20):. (PMID: 36293110)
J Cell Mol Med. 2020 Oct;24(20):12154-12163. (PMID: 32918330)
PLoS One. 2017 Jan 23;12(1):e0170628. (PMID: 28114422)
Braz J Med Biol Res. 2017 Apr 03;50(4):e5533. (PMID: 28380212)
BMC Mol Biol. 2006 Jan 31;7:3. (PMID: 16448564)
معلومات مُعتمدة: NA Il Sogno di Stefano
فهرسة مساهمة: Keywords: biomarkers; extracellular vesicle; microRNA; protocol biopsy; renal transplant; subclinical rejection
المشرفين على المادة: 0 (MicroRNAs)
0 (Biomarkers)
تواريخ الأحداث: Date Created: 20240210 Date Completed: 20240214 Latest Revision: 20240214
رمز التحديث: 20240214
مُعرف محوري في PubMed: PMC10856071
DOI: 10.3390/ijms25031911
PMID: 38339187
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms25031911