دورية أكاديمية
Comparative analysis of miRNA expression profiles in flowering and non-flowering tissue of Crocus sativus L.
| العنوان: | Comparative analysis of miRNA expression profiles in flowering and non-flowering tissue of Crocus sativus L. |
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| المؤلفون: | Bhat A; Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu, 180006, Jammu and Kashmir, India., Mishra S; Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu, 180006, Jammu and Kashmir, India., Kaul S; Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu, 180006, Jammu and Kashmir, India., Dhar MK; Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu, 180006, Jammu and Kashmir, India. manojkdhar@rediffmail.com. |
| المصدر: | Protoplasma [Protoplasma] 2024 Jul; Vol. 261 (4), pp. 749-769. Date of Electronic Publication: 2024 Feb 10. |
| نوع المنشور: | Journal Article; Comparative Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Austria NLM ID: 9806853 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-6102 (Electronic) Linking ISSN: 0033183X NLM ISO Abbreviation: Protoplasma Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <1998->: Wien ; New York : Springer Original Publication: Leipzig : Verlag von Gebrüder Borntraeger, 1927- |
| مواضيع طبية MeSH: | MicroRNAs*/genetics , MicroRNAs*/metabolism , Flowers*/genetics , Flowers*/growth & development , Flowers*/metabolism , Crocus*/genetics , Gene Expression Regulation, Plant*, Gene Expression Profiling ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism |
| مستخلص: | Crocus sativus is a valuable plant due to the presence of apocarotenoids in its stigma. Considerable work has been done in the past to understand the apocarotenoid biosynthetic pathway in saffron. However, the reports on understanding the regulation of flowering at the post-transcriptional level are meagre. The study aimed to discover the candidate miRNAs, target genes, transcription factors (TFs), and apocarotenoid biosynthetic pathway genes associated with the regulation and transition of flowering in C. sativus. In the present investigation, miRNA profiling was performed in flowering and non-flowering corms of saffron, along with expression analysis of apocarotenoid genes and transcription factors involved in the synthesis of secondary metabolites. Significant modulation in the expression of miR156, miR159, miR166, miR172, miR395, miR396, miR399, and miR408 gene families was observed. We obtained 36 known miRNAs (26 in flowering and 10 in non-flowering) and 64 novel miRNAs (40 in flowering and 24 in non-flowering) unique to specific tissues in our analysis. TFs, including CsMADS and CsMYb, showed significant modulation in expression in flowering tissue, followed by CsHB. Additionally, the miRNAs were predicted to be involved in carbohydrate metabolism, phytohormone signalling, regulation of flower development, and response to stress, cold, and defence. The comprehensive study has enhanced our understanding of the regulatory machinery comprising factors like phytohormones, abiotic stress, apocarotenoid genes, transcription factors, and miRNAs responsible for the synthesis of apocarotenoids and developmental processes during and after flowering. (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
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| معلومات مُعتمدة: | BT/PR5397/PBD/16/974/ 2012 DBT; BT/PR5409/PBD/16/977/2012 DBT |
| فهرسة مساهمة: | Keywords: Crocus sativus; Apocarotenoid pathway; Flowering; Regulation; Transcription factor; miRNA |
| المشرفين على المادة: | 0 (MicroRNAs) 0 (Transcription Factors) 0 (Plant Proteins) |
| تواريخ الأحداث: | Date Created: 20240210 Date Completed: 20240624 Latest Revision: 20240624 |
| رمز التحديث: | 20240624 |
| DOI: | 10.1007/s00709-024-01931-4 |
| PMID: | 38340171 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1615-6102 |
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| DOI: | 10.1007/s00709-024-01931-4 |