دورية أكاديمية
أعرض في EDS

The Macrophage Landscape Across the Lifespan of a Human Cardiac Allograft.

التفاصيل البيبلوغرافية
العنوان: The Macrophage Landscape Across the Lifespan of a Human Cardiac Allograft.
المؤلفون: Li X; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.)., Turaga D; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.).; Division of Critical Care Medicine (D.T.), Texas Children's Hospital, Houston TX., Li RG; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.)., Tsai CR; Department of Integrative Physiology (C.-R.T., K.C., J.F.M.), Baylor College of Medicine, Houston, TX., Quinn JN; Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston (J.N.Q., J.W.)., Zhao Y; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.)., Wilson R; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.)., Carlson K; Department of Integrative Physiology (C.-R.T., K.C., J.F.M.), Baylor College of Medicine, Houston, TX., Wang J; Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston (J.N.Q., J.W.)., Spinner JA; Department of Pediatrics (D.T., J.A.S.), Baylor College of Medicine, Houston, TX.; Division of Cardiology (J.A.S.), Texas Children's Hospital, Houston TX., Hickey EJ; Department of Surgery (E.J.H., I.A.), Baylor College of Medicine, Houston, TX.; Division of Congenital Heart Surgery (E.J.H., I.A.), Texas Children's Hospital, Houston TX., Adachi I; Department of Surgery (E.J.H., I.A.), Baylor College of Medicine, Houston, TX.; Division of Congenital Heart Surgery (E.J.H., I.A.), Texas Children's Hospital, Houston TX., Martin JF; The Texas Heart Institute, Houston (X.L., R.G.L., Y.Z., R.W., J.F.M.).; Department of Integrative Physiology (C.-R.T., K.C., J.F.M.), Baylor College of Medicine, Houston, TX.; Center for Organ Repair and Renewal (J.F.M.), Baylor College of Medicine, Houston, TX.
المصدر: Circulation [Circulation] 2024 May 21; Vol. 149 (21), pp. 1650-1666. Date of Electronic Publication: 2024 Feb 12.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0147763 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1524-4539 (Electronic) Linking ISSN: 00097322 NLM ISO Abbreviation: Circulation Subsets: MEDLINE
أسماء مطبوعة: Publication: Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: [Dallas, Tex., etc., American Heart Association, etc.]
مواضيع طبية MeSH: Heart Transplantation*/adverse effects , Macrophages*/metabolism , Allografts* , Graft Rejection*/immunology , Graft Rejection*/genetics, Humans ; Male ; Female ; Child ; Child, Preschool ; Myocardium/pathology ; Graft Survival ; Infant ; Killer Cells, Natural/immunology ; Killer Cells, Natural/metabolism ; Adolescent
مستخلص: Background: Much of our knowledge of organ rejection after transplantation is derived from rodent models.
Methods: We used single-nucleus RNA sequencing to investigate the inflammatory myocardial microenvironment in human pediatric cardiac allografts at different stages after transplantation. We distinguished donor- from recipient-derived cells using naturally occurring genetic variants embedded in single-nucleus RNA sequencing data.
Results: Donor-derived tissue resident macrophages, which accompany the allograft into the recipient, are lost over time after transplantation. In contrast, monocyte-derived macrophages from the recipient populate the heart within days after transplantation and form 2 macrophage populations: recipient MP1 and recipient MP2. Recipient MP2s have cell signatures similar to donor-derived resident macrophages; however, they lack signatures of pro-reparative phagocytic activity typical of donor-derived resident macrophages and instead express profibrotic genes. In contrast, recipient MP1s express genes consistent with hallmarks of cellular rejection. Our data suggest that recipient MP1s activate a subset of natural killer cells, turning them into a cytotoxic cell population through feed-forward signaling between recipient MP1s and natural killer cells.
Conclusions: Our findings reveal an imbalance of donor-derived and recipient-derived macrophages in the pediatric cardiac allograft that contributes to allograft failure.
Competing Interests: Disclosures None.
التعليقات: Comment in: Circulation. 2024 May 21;149(21):1667-1669. doi: 10.1161/CIRCULATIONAHA.124.068884. (PMID: 38768276)
References: Circulation. 2021 Apr 20;143(16):1614-1628. (PMID: 33682422)
Immunity. 2021 Sep 14;54(9):2057-2071.e6. (PMID: 34363749)
Genome Biol. 2014;15(12):550. (PMID: 25516281)
Circulation. 2022 Aug 23;146(8):623-638. (PMID: 35880523)
J Immunol. 2004 Sep 15;173(6):3953-61. (PMID: 15356144)
Nat Methods. 2017 Nov;14(11):1083-1086. (PMID: 28991892)
Adv Immunol. 2005;86:209-39. (PMID: 15705423)
Cell Syst. 2019 Apr 24;8(4):281-291.e9. (PMID: 30954476)
Nat Methods. 2020 Jun;17(6):615-620. (PMID: 32366989)
Proc Natl Acad Sci U S A. 2015 Feb 3;112(5):1535-40. (PMID: 25605927)
J Mol Med (Berl). 2017 Jul;95(7):767-777. (PMID: 28357477)
BMC Genomics. 2018 Jun 19;19(1):477. (PMID: 29914354)
Bioinformatics. 2016 Apr 15;32(8):1241-3. (PMID: 26668002)
Immunity. 2018 Aug 21;49(2):326-341.e7. (PMID: 30054204)
Sci Immunol. 2022 Jan 07;7(67):eabf7777. (PMID: 34995099)
Nature. 2018 Aug;560(7719):494-498. (PMID: 30089906)
Nat Med. 2005 Oct;11(10):1059-65. (PMID: 16155578)
J Immunol. 2015 Dec 15;195(12):5637-47. (PMID: 26561547)
Nat Methods. 2020 Jun;17(6):621-628. (PMID: 32424270)
Cell Death Dis. 2021 Sep 25;12(10):877. (PMID: 34564708)
OMICS. 2012 May;16(5):284-7. (PMID: 22455463)
Eur J Immunol. 2001 Mar;31(3):792-801. (PMID: 11241284)
Cell. 2021 Jun 24;184(13):3573-3587.e29. (PMID: 34062119)
J Am Soc Nephrol. 2020 Sep;31(9):1977-1986. (PMID: 32669324)
J Heart Lung Transplant. 2022 Jun;41(6):840-848. (PMID: 35317953)
Circ Res. 2016 Sep 16;119(7):853-64. (PMID: 27444755)
Circ Res. 2019 Jan 18;124(2):263-278. (PMID: 30582448)
Nat Commun. 2021 Feb 17;12(1):1088. (PMID: 33597522)
Cell. 2020 Oct 1;183(1):94-109.e23. (PMID: 32937105)
Transplantation. 2015 Dec;99(12):2467-75. (PMID: 26285017)
Nat Rev Immunol. 2006 Oct;6(10):751-60. (PMID: 16998508)
Science. 2022 May 13;376(6594):eabl4290. (PMID: 35549429)
Nat Biotechnol. 2018 Jun;36(5):411-420. (PMID: 29608179)
Nat Cardiovasc Res. 2022 Mar;1(3):263-280. (PMID: 35959412)
Nature. 2020 Dec;588(7838):466-472. (PMID: 32971526)
Nature. 2022 Aug;608(7924):766-777. (PMID: 35948637)
Nat Med. 2018 Aug;24(8):1234-1245. (PMID: 29892064)
J Leukoc Biol. 2000 May;67(5):725-34. (PMID: 10811014)
J Clin Invest. 2020 Apr 1;130(4):1629-1631. (PMID: 32175921)
Protein Cell. 2016 Mar;7(3):201-9. (PMID: 26874522)
Front Immunol. 2020 Mar 03;11:232. (PMID: 32194548)
Circ Heart Fail. 2023 Jan;16(1):e010119. (PMID: 36524467)
Arterioscler Thromb Vasc Biol. 2017 Aug;37(8):1494-1502. (PMID: 28596376)
Nat Biotechnol. 2018 Jan;36(1):89-94. (PMID: 29227470)
Nature. 2022 Aug;608(7921):181-191. (PMID: 35732239)
J Immunol. 2011 Nov 1;187(9):4835-43. (PMID: 21930960)
Proc Natl Acad Sci U S A. 2022 Apr 19;119(16):e2119168119. (PMID: 35412885)
Immunity. 2014 Jan 16;40(1):91-104. (PMID: 24439267)
Acta Biomater. 2014 Jun;10(6):2684-92. (PMID: 24561712)
Mol Syst Biol. 2021 Jan;17(1):e9620. (PMID: 33491336)
J Heart Lung Transplant. 2019 Oct;38(10):1056-1066. (PMID: 31548031)
Am J Transplant. 2018 Feb;18(2):289-292. (PMID: 28722285)
Blood Rev. 2006 May;20(3):123-37. (PMID: 16364519)
Science. 2022 Aug 5;377(6606):eabo1984. (PMID: 35926050)
معلومات مُعتمدة: R01 HL142704 United States HL NHLBI NIH HHS; R01 HL118761 United States HL NHLBI NIH HHS; R56 HL142704 United States HL NHLBI NIH HHS; R01 HL127717 United States HL NHLBI NIH HHS; R01 HL130804 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: allografts; heart; killer cells, natural; macrophages; pediatrics; tissue donor; transplant recipients
تواريخ الأحداث: Date Created: 20240212 Date Completed: 20240520 Latest Revision: 20240607
رمز التحديث: 20240607
مُعرف محوري في PubMed: PMC11105989
DOI: 10.1161/CIRCULATIONAHA.123.065294
PMID: 38344825
قاعدة البيانات: MEDLINE
الوصف
تدمد:1524-4539
DOI:10.1161/CIRCULATIONAHA.123.065294