دورية أكاديمية
Metagenomics for Pathogen Detection During a Mass Mortality Event in Songbirds.
| العنوان: | Metagenomics for Pathogen Detection During a Mass Mortality Event in Songbirds. |
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| المؤلفون: | Mwakibete L; Chan Zuckerberg Biohub, San Francisco, California 94158, USA., Greening SS; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA., Kalantar K; Chan Zuckerberg Initiative, Redwood City, California 94063, USA., Ahyong V; Chan Zuckerberg Biohub, San Francisco, California 94158, USA., Anis E; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA.; Department of Pathobiology, PADLS New Bolton Center, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA., Miller EA; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA., Needle DB; New Hampshire Veterinary Diagnostic Lab, University of New Hampshire, Durham, New Hampshire 03824, USA., Oglesbee M; Infectious Diseases Institute, The Ohio State University, Columbus, Ohio 43210, USA., Thomas WK; Hubbard Center for Genome Studies, University of New Hampshire, Durham, New Hampshire 03824, USA., Sevigny JL; Hubbard Center for Genome Studies, University of New Hampshire, Durham, New Hampshire 03824, USA., Gordon LM; Hubbard Center for Genome Studies, University of New Hampshire, Durham, New Hampshire 03824, USA., Nemeth NM; Southeastern Cooperative Wildlife Disease Study and Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.; Department of Pathology, College of Veterinary Medicine, University of Georgia, Georgia 30602, USA., Ogbunugafor CB; Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA., Ayala AJ; Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA., Faith SA; Infectious Diseases Institute, The Ohio State University, Columbus, Ohio 43210, USA., Neff N; Chan Zuckerberg Biohub, San Francisco, California 94158, USA., Detweiler AM; Chan Zuckerberg Biohub, San Francisco, California 94158, USA., Baillargeon T; New Hampshire Veterinary Diagnostic Lab, University of New Hampshire, Durham, New Hampshire 03824, USA., Tanguay S; New Hampshire Veterinary Diagnostic Lab, University of New Hampshire, Durham, New Hampshire 03824, USA., Simpson SD; Hubbard Center for Genome Studies, University of New Hampshire, Durham, New Hampshire 03824, USA., Murphy LA; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA.; Department of Pathobiology, PADLS New Bolton Center, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA., Ellis JC; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA., Tato CM; Chan Zuckerberg Biohub, San Francisco, California 94158, USA., Gagne RB; Department of Pathobiology, Wildlife Futures Program, University of Pennsylvania School of Veterinary Medicine, New Bolton Center, Kennett Square, Pennsylvania 19348, USA. |
| المصدر: | Journal of wildlife diseases [J Wildl Dis] 2024 Apr 01; Vol. 60 (2), pp. 362-374. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wildlife Disease Association Country of Publication: United States NLM ID: 0244160 Publication Model: Print Cited Medium: Internet ISSN: 1943-3700 (Electronic) Linking ISSN: 00903558 NLM ISO Abbreviation: J Wildl Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Lawrence, KS : Wildlife Disease Association Original Publication: Ames, Iowa, Wildlife Disease Assn. |
| مواضيع طبية MeSH: | Songbirds* , Communicable Diseases, Emerging*/veterinary, Animals ; Animals, Wild ; Metagenome ; Bacteria/genetics ; Metagenomics/methods |
| مستخلص: | Mass mortality events in wildlife can be indications of an emerging infectious disease. During the spring and summer of 2021, hundreds of dead passerines were reported across the eastern US. Birds exhibited a range of clinical signs including swollen conjunctiva, ocular discharge, ataxia, and nystagmus. As part of the diagnostic investigation, high-throughput metagenomic next-generation sequencing was performed across three molecular laboratories on samples from affected birds. Many potentially pathogenic microbes were detected, with bacteria forming the largest proportion; however, no singular agent was consistently identified, with many of the detected microbes also found in unaffected (control) birds and thus considered to be subclinical infections. Congruent results across laboratories have helped drive further investigation into alternative causes, including environmental contaminants and nutritional deficiencies. This work highlights the utility of metagenomic approaches in investigations of emerging diseases and provides a framework for future wildlife mortality events. (© Wildlife Disease Association 2024.) |
| فهرسة مساهمة: | Keywords: Avian; metagenomics; mortality event; rapid response; wildlife |
| تواريخ الأحداث: | Date Created: 20240212 Date Completed: 20240408 Latest Revision: 20240606 |
| رمز التحديث: | 20240606 |
| DOI: | 10.7589/JWD-D-23-00109 |
| PMID: | 38345467 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-3700 |
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| DOI: | 10.7589/JWD-D-23-00109 |