دورية أكاديمية
miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose.
| العنوان: | miR-140-y targets TCF4 to regulate the Wnt signaling pathway and promote embryonic feather follicle development in Hungarian white goose. |
|---|---|
| المؤلفون: | Ma J; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Song Y; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Mabrouk I; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Zhou Y; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Liu Q; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Yu J; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Li X; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Xue G; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Wang J; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Yu Z; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China., Sun Y; College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China; Key Laboratory of Animal Production, Product Quality and Security, Jilin Agricultural University, Ministry of Education, Changchun, 130118, China; Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. Electronic address: sunyongfeng@jlau.edu.cn. |
| المصدر: | Poultry science [Poult Sci] 2024 Apr; Vol. 103 (4), pp. 103508. Date of Electronic Publication: 2024 Feb 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0401150 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-3171 (Electronic) Linking ISSN: 00325791 NLM ISO Abbreviation: Poult Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Cambridge, UK] : Elsevier Original Publication: Champaign Il : Poultry Science Association |
| مواضيع طبية MeSH: | Wnt Signaling Pathway* , MicroRNAs*/genetics , MicroRNAs*/metabolism, Animals ; Geese/genetics ; Geese/metabolism ; Chickens/genetics ; Feathers ; Hungary ; Embryonic Development ; Cell Proliferation/genetics |
| مستخلص: | Goose down feather has become one of the most important economical products in the goose breeding industry and it provides several essential physiological roles in birds. Therefore, understanding and regulating the development of skin and feather follicles during embryogenesis is critical for avian biology and the poultry industry. MicroRNAs are known to play an important role in controlling gene expression during skin and feather follicle development. In this study, bioinformatics analysis was conducted to select miR-140-y as a potential miRNA involved in skin and feather follicle development and to predict TCF4 as its target gene. This gene was expressed at significant levels during embryonic feather follicle development, as identified by qPCR and Western blot. The targeting relationship was confirmed by a dual-luciferase assay in 293T cells. Then, the miR-140-y/TCF4 function in dermal fibroblast cells was explored. The results showed that miR-140-y could suppress the proliferation of goose embryonic dermal fibroblast cells (GEDFs) by suppressing the activity of some Wingless-types (Wnt) pathway related genes and proliferation marker genes, while miR-140-y inhibition led to the opposite effect. Similarly, the inhibition of the TCF4 gene results in blocking the proliferation of GEDFs by reducing the activity of some Wnt pathway-related genes. Finally, the co-transfection of miR-140-y inhibitor and siRNA-TCF4 results in a rescue of the TCF4 function and an increase of the Wnt signaling pathway and GEDFs proliferation. In conclusion, these results demonstrated that the miR-140-y-TCF4 axis influences the activity of the Wnt signaling pathway and works as a dynamic regulator during skin and feather follicle development. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: TCF4; Wnt/β-catenin signaling; dermal fibroblast cell; feather follicle; miR-140-y |
| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20240213 Date Completed: 20240322 Latest Revision: 20240322 |
| رمز التحديث: | 20240322 |
| مُعرف محوري في PubMed: | PMC10874752 |
| DOI: | 10.1016/j.psj.2024.103508 |
| PMID: | 38350393 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-3171 |
|---|---|
| DOI: | 10.1016/j.psj.2024.103508 |