دورية أكاديمية
أعرض في EDS

Prevalence, antimicrobial susceptibility test and associated factors of Salmonella and Shigella in ready-to-eat fruit juices and salads in Mekelle, northern Ethiopia.

التفاصيل البيبلوغرافية
العنوان: Prevalence, antimicrobial susceptibility test and associated factors of Salmonella and Shigella in ready-to-eat fruit juices and salads in Mekelle, northern Ethiopia.
المؤلفون: Berihu T; Department of Medical Laboratory Science, College of Health Science and Comprehensive Specialized Hospital, Aksum University, Axum, Tigray, Ethiopia., Gebremariam G; Department of Medical Laboratory Science, College of Health Science and Comprehensive Specialized Hospital, Aksum University, Axum, Tigray, Ethiopia. gueshgebre60@gmail.com., Weldu Y; Department of Medical Microbiology and Immunology, College of Health Science and Comprehensive Specialized Hospital, Mekelle University, Mekelle, Tigray, Ethiopia., Kahsay A; Department of Medical Microbiology and Immunology, College of Health Science and Comprehensive Specialized Hospital, Mekelle University, Mekelle, Tigray, Ethiopia., Asmelash T; Department of Medical Microbiology and Immunology, College of Health Science and Comprehensive Specialized Hospital, Mekelle University, Mekelle, Tigray, Ethiopia., Gebreyesus A; Department of Medical Microbiology and Immunology, College of Health Science and Comprehensive Specialized Hospital, Mekelle University, Mekelle, Tigray, Ethiopia.
المصدر: BMC infectious diseases [BMC Infect Dis] 2024 Feb 13; Vol. 24 (1), pp. 191. Date of Electronic Publication: 2024 Feb 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100968551 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2334 (Electronic) Linking ISSN: 14712334 NLM ISO Abbreviation: BMC Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Salads* , Shigella* , Anti-Infective Agents*, Amoxicillin-Potassium Clavulanate Combination ; Ethiopia/epidemiology ; Prevalence ; Cross-Sectional Studies ; Agar ; Fruit and Vegetable Juices ; Microbial Sensitivity Tests ; Salmonella ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Ampicillin ; Tetracycline ; Dust
مستخلص: Background: Food borne diseases is a challenging problem nowadays. Salmonella and Shigella species are great concern of food-born outbreaks. Thus, this study was aimed to assess the prevalence, antimicrobial susceptibility test and associated factors of Salmonella and Shigella species in fruit juices and salads.
Methods: A community based cross sectional study design was carried out on 50 juice houses from December to March 2020 in Mekelle. One hundred fifty samples were collected aseptically from the juice houses for laboratory analysis. Information related to risk factors was obtained using a structured questionnaire. In the laboratory, samples were homogenized using peptone water and incubated overnight for enrichment. Then, Salmonella and Shigella species were isolated on Salmonella-Shigella agar and Xylose Lysine Deoxycholate agar. Disc diffusion method was used to perform antimicrobial susceptibility test. Using SPSS (version 22) package, descriptive statistics and Chi square test (χ2) were used to analyze the data, and p < 0.05 was considered as statistically significant.
Result: The overall prevalence of Salmonella and Shigella species was 41/150 (27.33%; 95% CI: 20.20 - 34.46) with 33 (22%) Salmonella spp. and 8(5.33%) Shigella spp. Antimicrobial susceptibility tests of both Salmonella and Shigella spp.showed high resistance against ampicillin (100%), tetracycline (63.6 and 62.5%, respectively) and amoxicillin-clavulanic acid (100%). Accessibility of fruits to flies and dust had statistical association (p = 0.021) with occurrence of Salmonell a and/or Shigella spp.
Conclusion: The overall prevalence of Salmonella and Shigella spp. was found to be significant. The resistant rate of isolates against ampicillin, tetracycline and amoxicillin-clavulanic acid was high. Storage sites for fruits should be inaccessible to flies and dust. Therefore, routine monitoring of juice houses should be promoted and regular evaluation of bacterial resistance pattern should be done for selective antimicrobial therapy. Furthermore, consistent training of juice makers on food safety and hygiene should be implemented by the concerned body.
(© 2024. The Author(s).)
References: Biol Res. 2010;43(2):141-8. (PMID: 21031258)
J Antimicrob Chemother. 2004 Aug;54(2):311-20. (PMID: 15215223)
Ethiop J Health Sci. 2012 Jul;22(2):121-8. (PMID: 22876076)
Ethiop J Health Sci. 2010 Mar;20(1):41-8. (PMID: 22434959)
Int J Environ Res Public Health. 2013 Jun 28;10(7):2643-69. (PMID: 23812024)
Int J Food Sci. 2014;2014:408085. (PMID: 26904628)
J Appl Microbiol. 2007 Jul;103(1):219-27. (PMID: 17584468)
Int J Food Microbiol. 2015 Aug 3;206:1-6. (PMID: 25910073)
Ethiop J Health Sci. 2016 Nov;26(6):533-542. (PMID: 28450768)
Jpn J Infect Dis. 2009 Mar;62(2):93-7. (PMID: 19305047)
Immunol Cell Biol. 2007 Feb-Mar;85(2):112-8. (PMID: 17146467)
Virulence. 2011 Nov-Dec;2(6):580-92. (PMID: 22030860)
Ethiop J Health Sci. 2013 Mar;23(1):19-26. (PMID: 23559834)
فهرسة مساهمة: Keywords: Antimicrobial susceptibility; Fruit juice; Salad; Salmonella; Shigella
المشرفين على المادة: 74469-00-4 (Amoxicillin-Potassium Clavulanate Combination)
9002-18-0 (Agar)
0 (Anti-Bacterial Agents)
7C782967RD (Ampicillin)
0 (Anti-Infective Agents)
F8VB5M810T (Tetracycline)
0 (Dust)
تواريخ الأحداث: Date Created: 20240213 Date Completed: 20240215 Latest Revision: 20240217
رمز التحديث: 20240217
مُعرف محوري في PubMed: PMC10863215
DOI: 10.1186/s12879-024-09066-w
PMID: 38350855
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2334
DOI:10.1186/s12879-024-09066-w