دورية أكاديمية
"MeiQuant": An Integrated Tool for Analyzing Meiotic Prophase I Spread Images.
| العنوان: | "MeiQuant": An Integrated Tool for Analyzing Meiotic Prophase I Spread Images. |
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| المؤلفون: | Cau J; Biocampus Montpellier, University of Montpellier, CNRS, INSERM, Montpellier, France. julien.cau@biocampus.cnrs.fr., Toe LD; CBS (Centre de Biologie Structurale), Univ Montpellier, CNRS, INSERM, Montpellier, France., Zainu A; Institut de Génétique Humaine, University of Montpellier, CNRS, Montpellier, France., Baudat F; Institut de Génétique Humaine, University of Montpellier, CNRS, Montpellier, France. frederic.baudat@igh.cnrs.fr., Robert T; CBS (Centre de Biologie Structurale), Univ Montpellier, CNRS, INSERM, Montpellier, France. thomas.robert@cbs.cnrs.fr. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2770, pp. 263-285. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Meiosis* , Meiotic Prophase I*, Animals ; Mice ; Prophase ; Chromosomes |
| مستخلص: | Immunocytochemical analysis of meiotic proteins on mouse chromosome spreads is one method of choice to study prophase I chromosome organization and homologous recombination. In recent decades, the development of microscopic approaches led to the production of a large number of images that monitor fluorescent proteins, defined as fluorescent objects, and a major challenge facing the community is the deep analysis of these fluorescent objects (measurement of object length, intensity, distance between objects, as well as foci identification, counting, and colocalization). We propose a set of tools designed from the macro language of the widely used image analysis software ImageJ (Schindelin et al., Nat Methods 9: 676-682, 2012), embedded in the "MeiQuant" macro, which are specifically designed for analyzing objects in the field of meiosis. Our aim is to propose a unified evolutive common tool for image analysis, with a specific focus on mouse prophase I meiotic events. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Chromosome structure; Foci count; Homologous recombination; Image analysis; ImageJ; Intensity measurement; MeiQuant; Meiosis; Protein localization |
| تواريخ الأحداث: | Date Created: 20240214 Date Completed: 20240215 Latest Revision: 20240215 |
| رمز التحديث: | 20240215 |
| DOI: | 10.1007/978-1-0716-3698-5_17 |
| PMID: | 38351458 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3698-5_17 |