دورية أكاديمية
Mechanisms on salt tolerant of Paenibacillus polymyxa SC2 and its growth-promoting effects on maize seedlings under saline conditions.
| العنوان: | Mechanisms on salt tolerant of Paenibacillus polymyxa SC2 and its growth-promoting effects on maize seedlings under saline conditions. |
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| المؤلفون: | Wang C; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China. Electronic address: wangcq@sdau.edu.cn., Pei J; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Li H; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Zhu X; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Zhang Y; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Wang Y; Institute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Li W; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Wang Z; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Liu K; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Du B; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China., Jiang J; Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin 150030, China. Electronic address: jjqdainty@163.com., Zhao D; College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University, Tai'an 271018, China; College of Life Sciences, Dezhou University, Dezhou 253023, China. Electronic address: zhaodongying4321@163.com. |
| المصدر: | Microbiological research [Microbiol Res] 2024 May; Vol. 282, pp. 127639. Date of Electronic Publication: 2024 Feb 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: G. Fischer Country of Publication: Germany NLM ID: 9437794 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-0623 (Electronic) Linking ISSN: 09445013 NLM ISO Abbreviation: Microbiol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Jena : G. Fischer, c1994- |
| مواضيع طبية MeSH: | Seedlings*/microbiology , Paenibacillus polymyxa*/genetics, Zea mays/microbiology ; Soil ; Ethylenes/metabolism |
| مستخلص: | Soil salinity negatively affects microbial communities, soil fertility, and agricultural productivity and has become a major agricultural problem worldwide. Plant growth-promoting rhizobacteria (PGPR) with salt tolerance can benefit plant growth under saline conditions and diminish the negative effects of salt stress on plants. In this study, we aimed to understand the salt-tolerance mechanism of Paenibacillus polymyxa at the genetic and metabolic levels and elucidate the mechanism of strain SC2 in promoting maize growth under saline conditions. Under salt stress, we found that strain SC2 promoted maize seedling growth, which was accompanied by a significant upregulation of genes encoding for the biosynthesis of peptidoglycan, polysaccharide, and fatty acid, the metabolism of purine and pyrimidine, and the transport of osmoprotectants such as trehalose, glycine betaine, and K + in strain SC2. To further enhance the salt resistance of strain SC2, three mutants (SC2-11, SC2-13, and SC2-14) with higher capacities for salt resistance and exopolysaccharide synthesis were obtained via atmospheric and room-temperature plasma mutagenesis. In saline-alkaline soil, the mutants showed better promoting effect on maize seedlings than wild-type SC2. The fresh weight of maize seedlings was increased by 68.10% after treatment with SC2-11 compared with that of the control group. The transcriptome analysis of maize roots demonstrated that SC2 and SC2-11 could induce the upregulation of genes related to the plant hormone signal transduction, starch and sucrose metabolism, reactive oxygen species scavenging, and auxin and ethylene signaling under saline-alkaline stress. In addition, various transcription factors, such as zinc finger proteins, ethylene-responsive-element-binding protein, WRKY, myeloblastosis proteins, basic helix-loop-helix proteins, and NAC proteins, were up-regulated in response to abiotic stress. Moreover, the microbial community composition of maize rhizosphere soil after inoculating with strain SC2 was varied from the one after inoculating with mutant SC2-11. Our results provide new insights into the various genes involved in the salt resistance of strain SC2 and a theoretical basis for utilizing P. polymyxa in saline-alkaline environments. Competing Interests: Declaration of competing interest All the authors declare that there are no conflicts of interest. (Copyright © 2024 Elsevier GmbH. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Maize; Paenibacillus polymyxa; Salt-tolerance; Transcriptome |
| المشرفين على المادة: | 0 (Soil) 0 (Ethylenes) |
| تواريخ الأحداث: | Date Created: 20240214 Date Completed: 20240318 Latest Revision: 20240318 |
| رمز التحديث: | 20240318 |
| DOI: | 10.1016/j.micres.2024.127639 |
| PMID: | 38354626 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1618-0623 |
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| DOI: | 10.1016/j.micres.2024.127639 |