دورية أكاديمية
Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses.
| العنوان: | Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses. |
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| المؤلفون: | Mimura Y; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan., Hiono T; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.; One Health Research Center, Hokkaido University, Hokkaido, Japan.; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan.; Hokkaido University Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, Hokkaido, Japan., Huynh LT; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.; Faculty of Veterinary Medicine, College of Agriculture, Can Tho University, Can Tho, Vietnam., Ogino S; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan., Kobayashi M; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan., Isoda N; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.; One Health Research Center, Hokkaido University, Hokkaido, Japan.; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan.; Hokkaido University Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, Hokkaido, Japan., Sakoda Y; Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.; One Health Research Center, Hokkaido University, Hokkaido, Japan.; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Hokkaido, Japan.; Hokkaido University Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, Hokkaido, Japan. |
| المصدر: | The Journal of veterinary medical science [J Vet Med Sci] 2024 Apr 01; Vol. 86 (4), pp. 389-395. Date of Electronic Publication: 2024 Feb 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Japanese Society Of Veterinary Science Country of Publication: Japan NLM ID: 9105360 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1347-7439 (Electronic) Linking ISSN: 09167250 NLM ISO Abbreviation: J Vet Med Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Tokyo : Japanese Society Of Veterinary Science |
| مواضيع طبية MeSH: | Pestivirus*/genetics , Superinfection*/veterinary , Classical Swine Fever Virus*/genetics , Swine Diseases*, Animals ; Swine ; Luciferases/genetics |
| مستخلص: | Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE - /HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. |
| References: | J Gen Virol. 1998 Dec;79 ( Pt 12):2981-7. (PMID: 9880012) J Virol. 2019 Oct 29;93(22):. (PMID: 31462560) J Virol. 1985 May;54(2):351-7. (PMID: 3989908) J Virol. 2000 Dec;74(24):11792-9. (PMID: 11090179) In Vitro Cell Dev Biol Anim. 1998 Jan;34(1):53-7. (PMID: 9542636) J Vet Med Sci. 2001 Jul;63(7):751-8. (PMID: 11503902) J Virol. 2018 Jan 2;92(2):. (PMID: 29093094) J Gen Virol. 2007 Oct;88(Pt 10):2730-2739. (PMID: 17872526) Virus Res. 2020 Jan 15;276:197809. (PMID: 31715204) J Virol. 2009 Nov;83(22):11765-76. (PMID: 19726510) Pathogens. 2020 Mar 04;9(3):. (PMID: 32143534) J Virol. 1998 Jun;72(6):5318-22. (PMID: 9573312) J Clin Microbiol. 2013 Aug;51(8):2710-2. (PMID: 23698523) J Virol Methods. 1998 Nov;75(1):59-68. (PMID: 9820575) J Gen Virol. 2003 Feb;84(Pt 2):447-452. (PMID: 12560578) J Virol. 1995 Jun;69(6):3683-9. (PMID: 7745717) Nature. 1989 Oct 12;341(6242):491. (PMID: 2552321) J Virol. 2005 Mar;79(6):3231-42. (PMID: 15731218) Virology. 1992 Nov;191(1):368-86. (PMID: 1329326) ACS Chem Biol. 2016 Feb 19;11(2):400-8. (PMID: 26569370) J Virol. 2016 Mar 28;90(8):4049-58. (PMID: 26842480) Curr Biol. 2005 Apr 26;15(8):714-23. (PMID: 15854903) J Virol. 2008 May;82(10):4834-43. (PMID: 18321971) Proc Soc Exp Biol Med. 1962 Jun;110:248-50. (PMID: 13898635) J Virol. 2003 Apr;77(7):4283-90. (PMID: 12634385) Antiviral Res. 2014 Sep;109:15-21. (PMID: 24956495) PLoS One. 2016 Feb 26;11(2):e0149469. (PMID: 26919741) Vet Microbiol. 1992 Nov;33(1-4):231-8. (PMID: 1336241) ACS Chem Biol. 2018 Feb 16;13(2):467-474. (PMID: 28892606) J Virol. 1994 May;68(5):3289-97. (PMID: 8151789) J Virol Methods. 2006 Dec;138(1-2):140-6. (PMID: 17046073) J Virol. 2007 Apr;81(8):3693-703. (PMID: 17287280) |
| فهرسة مساهمة: | Keywords: classical swine fever virus; infectivity titer; luciferase activity; pestivirus; superinfection exclusion |
| المشرفين على المادة: | EC 1.13.12.- (Luciferases) |
| تواريخ الأحداث: | Date Created: 20240214 Date Completed: 20240402 Latest Revision: 20240503 |
| رمز التحديث: | 20240503 |
| مُعرف محوري في PubMed: | PMC11061576 |
| DOI: | 10.1292/jvms.24-0005 |
| PMID: | 38355118 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1347-7439 |
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| DOI: | 10.1292/jvms.24-0005 |