دورية أكاديمية
Apium extract alleviates indomethacin-induced gastric ulcers in rats via modulating the VEGF and IK-κB/NF-κB p65 signaling pathway: insights from in silico and in vivo investigations.
| العنوان: | Apium extract alleviates indomethacin-induced gastric ulcers in rats via modulating the VEGF and IK-κB/NF-κB p65 signaling pathway: insights from in silico and in vivo investigations. |
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| المؤلفون: | Abu-Baih DH; Department of Biochemistry, Faculty of Pharmacy, Deraya University, New Minia, 61111, Egypt., Gomaa AA; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt., Abdel-Wahab NM; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt., Abdelaleem ER; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt., Zaher AMA; Department of Pathology, Faculty of Medicine, Minia University, Minia, Egypt., Hassan NF; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, 11571, Egypt., Bringmann G; Institute of Organic Chemistry, University of Würzburg, Am Hubland, Würzburg, 97074, Germany., Abdelmohsen UR; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt. usama.ramadan@mu.edu.eg.; Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Minia, 61111, Egypt. usama.ramadan@mu.edu.eg., Altemani FH; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia., Algehainy NA; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia., Mokhtar FA; Fujairah Research Centre, Sakamkam Road, Fujairah, United Arab Emirates.; Department of pharmacognosy, Faculty of pharmacy, El Saleheya El Gadida University, El Saleheya El Gadida, 44813, Sharkia, Egypt., Abdelwahab MF; Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt. |
| المصدر: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2024 Feb 14; Vol. 24 (1), pp. 88. Date of Electronic Publication: 2024 Feb 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2020]- |
| مواضيع طبية MeSH: | Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy , Apium*/metabolism , Anti-Ulcer Agents*/adverse effects, Rats ; Animals ; Indomethacin/adverse effects ; Vascular Endothelial Growth Factor A ; NF-kappa B/metabolism ; Plant Extracts/therapeutic use ; Signal Transduction |
| مستخلص: | Background: Gastric ulcers represent a worldwide health problem, characterized by erosions that affect the mucous membrane of the stomach and may even reach the muscular layer, leading to serious complications. Numerous natural products have been assessed as anti-ulcerogenic agents, and have been considered as new approaches for treatment or prevention of gastric ulcers. The present research investigated the preventive benefits of Apium graveolens L. (Apiaceae), known as celery, seed extract towards indomethacin-induced ulceration of the stomach in rats. Methods: Metabolomic profiling, employing liquid chromatography coupled to high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS), was implemented with the aim of investigating the chemical profile of the seeds. Histopathological analysis of gastric tissues, as well as assessment of numerous inflammatory cytokines and oxidative stress indicators, confirmed the in vivo evaluation. Results: The prior treatment with A. graveolens seed extract resulted in a substantial reduction in the ulcer index when compared to the indomethacin group, indicating an improvement in stomach mucosal injury. Moreover, the gastroprotective effect was demonstrated through examination of the oxidative stress biomarkers which was significantly attenuated upon pre-treatment with A. graveolens seed extract. Vascular endothelial growth factor (VEGF), a fundamental angiogenic factor that stimulates angiogenesis, was markedly inhibited by indomethacin. A. graveolens seed extract restored this diminished level of VEGF. The dramatic reductions in NF-κB protein levels indicate a considerable attenuation of the indomethacin-induced IKκB/NF-κB p65 signaling cascade. These activities were also correlated to the tentatively featured secondary metabolites including, phenolic acids, coumarins and flavonoids, previously evidenced to exert potent anti-inflammatory and antioxidant activities. According to our network pharmacology study, the identified metabolites annotated 379 unique genes, among which only 17 genes were related to gastric ulcer. The PTGS2, MMP2 and PTGS1 were the top annotated genes related to gastric ulcer. The top biological pathway was the VEGF signaling pathway. Conclusion: A. graveolens seed extract possesses significant anti-ulcer activity, similar to famotidine, against gastric lesions induced by indomethacin in rats. It is worth highlighting that the extract overcomes the negative effects of conventional chemical anti-secretory drugs because it does not lower stomach acidity. (© 2024. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Apium graveolens L.; Gastric ulcer; Inflammatory cytokines; Metabolomic profiling; Network pharmacology |
| المشرفين على المادة: | XXE1CET956 (Indomethacin) 0 (Vascular Endothelial Growth Factor A) 0 (NF-kappa B) 0 (Anti-Ulcer Agents) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20240214 Date Completed: 20240216 Latest Revision: 20240217 |
| رمز التحديث: | 20240217 |
| مُعرف محوري في PubMed: | PMC10865661 |
| DOI: | 10.1186/s12906-023-04333-w |
| PMID: | 38355510 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2662-7671 |
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| DOI: | 10.1186/s12906-023-04333-w |