دورية أكاديمية
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A two-dimensional iron-doped carbon-based nanoenzyme with catalase-like activity for the detection of alkaline phosphatase and ascorbate oxidase.

التفاصيل البيبلوغرافية
العنوان: A two-dimensional iron-doped carbon-based nanoenzyme with catalase-like activity for the detection of alkaline phosphatase and ascorbate oxidase.
المؤلفون: Han Z; Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China., Fu Q; Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China., Lv Y; Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China., Wang N; Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China., Su X; Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China. Electronic address: suxg@jlu.edu.cn.
المصدر: Talanta [Talanta] 2024 May 15; Vol. 272, pp. 125704. Date of Electronic Publication: 2024 Jan 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
مواضيع طبية MeSH: Alkaline Phosphatase* , Carbon*, Humans ; Ascorbate Oxidase ; Catalase ; Iron ; Reactive Oxygen Species ; Coloring Agents ; Limit of Detection
مستخلص: Herein, we successfully synthesized two-dimensional iron-doped carbon-based nanosheets (Fe-N 800 CS) with catalase-like activity through doping Fe into Zn MOF and introducing graphitic C 3 N 4 (g-C 3 N 4 ). The interaction of the Fe-N 800 CS with hydrogen peroxide could generated abundant reactive oxygen species (ROS) and further oxidize o-Phenylenediamine (OPD) to 2,3-diaminophenazine (DAP) which has constant fluorescence at 560 nm. Ascorbic acid (AA) could be generated via the hydrolysis reaction between alkaline phosphatase (ALP) and ascorbic acid 2-phosphate (AAP). AA can be oxidized to dehy-droascorbic acid (DHA) by ROS, and then combined with OPD to generate 3-(1,2-dihydroxyethyl)furo[3,4b]-quinoxaline (QXD) with fluorescence at 440 nm, which could increase as the concentration of AA enhanced. DHA could also be generated through oxidation of AA by ascorbate oxidase (AAO). Thus, by monitoring the fluorescence ratio (I 560 /I 440 ), a ratiometric fluorescence biosensing platform for ALP and AAO was established with the linear ranges in 0.2-10 U/L and 1-60 U/L, respectively. The limit of detection for ALP and AAO were 0.12 U/L and 0.59 U/L. Furthermore, the biosensing platform was successfully applied for the detection of ALP and AAO activity in human serum samples. This work provides a potential tool for future biomedical diagnostics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Alkaline phosphatase; Ascorbate oxidase; Metal-organic framework; Nanozyme; Ratiometric fluorescence
المشرفين على المادة: EC 3.1.3.1 (Alkaline Phosphatase)
7440-44-0 (Carbon)
EC 1.10.3.3 (Ascorbate Oxidase)
EC 1.11.1.6 (Catalase)
E1UOL152H7 (Iron)
0 (Reactive Oxygen Species)
0 (Coloring Agents)
تواريخ الأحداث: Date Created: 20240215 Date Completed: 20240318 Latest Revision: 20240318
رمز التحديث: 20240318
DOI: 10.1016/j.talanta.2024.125704
PMID: 38359716
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3573
DOI:10.1016/j.talanta.2024.125704