دورية أكاديمية
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Gene Editing of Candida glycerinogenes by Designed Toxin-Antitoxin Cassette.

التفاصيل البيبلوغرافية
العنوان: Gene Editing of Candida glycerinogenes by Designed Toxin-Antitoxin Cassette.
المؤلفون: Lv W; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.; Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi 214122, China., Lu X; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.; Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi 214122, China., Zhuge B; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.; Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi 214122, China., Zong H; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.; Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
المصدر: ACS synthetic biology [ACS Synth Biol] 2024 Mar 15; Vol. 13 (3), pp. 816-824. Date of Electronic Publication: 2024 Feb 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, c2012-
مواضيع طبية MeSH: Antitoxins*/genetics , Bacterial Toxins*/genetics , Phenylethyl Alcohol* , Pichia*, Gene Editing/methods ; Escherichia coli/genetics ; Escherichia coli/metabolism
مستخلص: Candida glycerinogenes is an industrial yeast with excellent multistress resistance. However, due to the diploid genome and the lack of meiosis and screening markers, its molecular genetic operation is limited. Here, a gene editing system using the toxin-antitoxin pair relBE from the type II toxin-antitoxin system in Escherichia coli as a screening marker was constructed. The RelBE complex can specifically and effectively regulate cell growth and arrest through a conditionally controlled toxin RelE switch, thereby achieving the selection of positive recombinants. The constructed editing system achieved precise gene deletion, replacement, insertion, and gene episomal expression in C. glycerinogenes . Compared with the traditional amino acid deficiency complementation editing system, this editing system produced higher biomass and the gene deletion efficiency was increased by 3.5 times. Using this system, the production of 2-phenylethanol by C. glycerinogenes was increased by 11.5-13.5% through metabolic engineering and tolerance engineering strategies. These results suggest that the stable gene editing system based on toxin-antitoxin pairs can be used for gene editing of C. glycerinogenes to modify metabolic pathways and promote industrial applications. Therefore, the constructed gene editing system is expected to provide a promising strategy for polyploid industrial microorganisms lacking gene manipulation methods.
فهرسة مساهمة: Keywords: Candida glycerinogenes; gene editing system; polyploid; relBE; toxin−antitoxin (TA) system
المشرفين على المادة: 0 (Antitoxins)
0 (Bacterial Toxins)
ML9LGA7468 (Phenylethyl Alcohol)
SCR Organism: Pichia kudriavzevii
تواريخ الأحداث: Date Created: 20240216 Date Completed: 20240318 Latest Revision: 20240620
رمز التحديث: 20240620
DOI: 10.1021/acssynbio.3c00640
PMID: 38365187
قاعدة البيانات: MEDLINE
الوصف
تدمد:2161-5063
DOI:10.1021/acssynbio.3c00640