دورية أكاديمية
Dynamic nitrogen fixation in an aerobic endophyte of Populus.
| العنوان: | Dynamic nitrogen fixation in an aerobic endophyte of Populus. |
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| المؤلفون: | Sher AW; School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, WA, 98195-2100, United States., Aufrecht JA; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Herrera D; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Zimmerman AE; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Kim YM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Munoz N; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Trejo JB; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Paurus VL; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Cliff JB; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Hu D; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Chrisler WB; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Tournay RJ; School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, WA, 98195-2100, United States., Gomez-Rivas E; School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, WA, 98195-2100, United States., Orr G; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Ahkami AH; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, United States., Doty SL; School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, WA, 98195-2100, United States. |
| المصدر: | The ISME journal [ISME J] 2024 Jan 08; Vol. 18 (1). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101301086 Publication Model: Print Cited Medium: Internet ISSN: 1751-7370 (Electronic) Linking ISSN: 17517362 NLM ISO Abbreviation: ISME J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2024- : Oxford : Oxford University Press Original Publication: London : Nature Pub. Group |
| مواضيع طبية MeSH: | Nitrogen Fixation*/physiology , Populus*/genetics , Populus*/metabolism, Endophytes/genetics ; Oxidoreductases/genetics ; In Situ Hybridization, Fluorescence ; Nitrogenase/genetics ; Nitrogenase/metabolism ; Nitrogen |
| مستخلص: | Biological nitrogen fixation by microbial diazotrophs can contribute significantly to nitrogen availability in non-nodulating plant species. In this study of molecular mechanisms and gene expression relating to biological nitrogen fixation, the aerobic nitrogen-fixing endophyte Burkholderia vietnamiensis, strain WPB, isolated from Populus trichocarpa served as a model for endophyte-poplar interactions. Nitrogen-fixing activity was observed to be dynamic on nitrogen-free medium with a subset of colonies growing to form robust, raised globular like structures. Secondary ion mass spectrometry (NanoSIMS) confirmed that N-fixation was uneven within the population. A fluorescent transcriptional reporter (GFP) revealed that the nitrogenase subunit nifH is not uniformly expressed across genetically identical colonies of WPB and that only ~11% of the population was actively expressing the nifH gene. Higher nifH gene expression was observed in clustered cells through monitoring individual bacterial cells using single-molecule fluorescence in situ hybridization. Through 15N2 enrichment, we identified key nitrogenous metabolites and proteins synthesized by WPB and employed targeted metabolomics in active and inactive populations. We cocultivated WPB Pnif-GFP with poplar within a RhizoChip, a synthetic soil habitat, which enabled direct imaging of microbial nifH expression within root epidermal cells. We observed that nifH expression is localized to the root elongation zone where the strain forms a unique physical interaction with the root cells. This work employed comprehensive experimentation to identify novel mechanisms regulating both biological nitrogen fixation and beneficial plant-endophyte interactions. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.) |
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| معلومات مُعتمدة: | #50774 Environmental Molecular Sciences Laboratory; United States Department of Energy Office of Science; DE-SC0021137 Office of Biological and Environmental Research |
| فهرسة مساهمة: | Keywords: 15 N-tracking metabolomics; 15 N-tracking proteomics; Burkholderia; Populus; endophyte; microbial ecology; nanoSIMS; nitrogen fixation; nitrogenase expression; plant–microbe interactions |
| المشرفين على المادة: | EC 1.- (Oxidoreductases) EC 1.18.6.1 (Nitrogenase) N762921K75 (Nitrogen) |
| تواريخ الأحداث: | Date Created: 20240216 Date Completed: 20240219 Latest Revision: 20240219 |
| رمز التحديث: | 20240219 |
| مُعرف محوري في PubMed: | PMC10833079 |
| DOI: | 10.1093/ismejo/wrad012 |
| PMID: | 38365250 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1751-7370 |
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| DOI: | 10.1093/ismejo/wrad012 |