دورية أكاديمية
CRISPR-Cas in Pseudomonas aeruginosa provides transient population-level immunity against high phage exposures.
| العنوان: | CRISPR-Cas in Pseudomonas aeruginosa provides transient population-level immunity against high phage exposures. |
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| المؤلفون: | Watson BNJ; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., Capria L; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., Alseth EO; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom.; Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, GA 30332, United States., Pons BJ; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., Biswas A; Department of Microbiology and Immunology, University of Otago, Dunedin, 9059, Otago, New Zealand., Lenzi L; Institute of Integrative Biology, University of Liverpool, Liverpool, Merseyside, L69 7BE, United Kingdom., Buckling A; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., van Houte S; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., Westra ER; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom., Meaden S; Biosciences, University of Exeter, Penryn, Cornwall, TR10 9FE, United Kingdom.; Department of Biology, University of York, Wentworth Way, York, North Yorkshire YO10 3DB, United Kingdom. |
| المصدر: | The ISME journal [ISME J] 2024 Jan 08; Vol. 18 (1). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101301086 Publication Model: Print Cited Medium: Internet ISSN: 1751-7370 (Electronic) Linking ISSN: 17517362 NLM ISO Abbreviation: ISME J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2024- : Oxford : Oxford University Press Original Publication: London : Nature Pub. Group |
| مواضيع طبية MeSH: | Bacteriophages*/genetics, Pseudomonas aeruginosa/genetics ; CRISPR-Cas Systems ; Bacteria/genetics ; Mutation |
| مستخلص: | The prokaryotic adaptive immune system, CRISPR-Cas (clustered regularly interspaced short palindromic repeats; CRISPR-associated), requires the acquisition of spacer sequences that target invading mobile genetic elements such as phages. Previous work has identified ecological variables that drive the evolution of CRISPR-based immunity of the model organism Pseudomonas aeruginosa PA14 against its phage DMS3vir, resulting in rapid phage extinction. However, it is unclear if and how stable such acquired immunity is within bacterial populations, and how this depends on the environment. Here, we examine the dynamics of CRISPR spacer acquisition and loss over a 30-day evolution experiment and identify conditions that tip the balance between long-term maintenance of immunity versus invasion of alternative resistance strategies that support phage persistence. Specifically, we find that both the initial phage dose and reinfection frequencies determine whether or not acquired CRISPR immunity is maintained in the long term, and whether or not phage can coexist with the bacteria. At the population genetics level, emergence and loss of CRISPR immunity are associated with high levels of spacer diversity that subsequently decline due to invasion of bacteria carrying pilus-associated mutations. Together, these results provide high resolution of the dynamics of CRISPR immunity acquisition and loss and demonstrate that the cumulative phage burden determines the effectiveness of CRISPR over ecologically relevant timeframes. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.) |
| معلومات مُعتمدة: | International ERC_ European Research Council; United Kingdom BB_ Biotechnology and Biological Sciences Research Council; United Kingdom WT_ Wellcome Trust; BB/N017412/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/X010600/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
| فهرسة مساهمة: | Keywords: CRISPR; ecological dynamics; evolution; immunity; phage |
| تواريخ الأحداث: | Date Created: 20240216 Date Completed: 20240223 Latest Revision: 20240717 |
| رمز التحديث: | 20240717 |
| مُعرف محوري في PubMed: | PMC10873826 |
| DOI: | 10.1093/ismejo/wrad039 |
| PMID: | 38366022 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1751-7370 |
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| DOI: | 10.1093/ismejo/wrad039 |