دورية أكاديمية
Different behavior of Ferguson plot between agarose and polyacrylamide gels.
| العنوان: | Different behavior of Ferguson plot between agarose and polyacrylamide gels. |
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| المؤلفون: | Tomioka Y; Product Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan. Electronic address: y.tomioka@kyokutoseiyaku.co.jp., Akuta T; Product Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan. Electronic address: t.akuta@kyokutoseiyaku.co.jp., Tokunaga M; Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima-shi 890-0065, Japan., Arakawa T; Alliance Protein Laboratories, 13380 Pantera Rd, San Diego, CA 92130, USA. Electronic address: tarakawa2@aol.com. |
| المصدر: | Biophysical chemistry [Biophys Chem] 2024 Apr; Vol. 307, pp. 107200. Date of Electronic Publication: 2024 Feb 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science B.V Country of Publication: Netherlands NLM ID: 0403171 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4200 (Electronic) Linking ISSN: 03014622 NLM ISO Abbreviation: Biophys Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science B.V Original Publication: Amsterdam, North-Holland Pub. Co. |
| مواضيع طبية MeSH: | Proteins* , Endopeptidase Clp* , Acrylic Resins*, Sepharose ; Electrophoresis, Polyacrylamide Gel ; Electrophoresis, Agar Gel/methods ; Gels |
| مستخلص: | In this study, we conducted Ferguson plot analyses using both agarose and polyacrylamide gels in native electrophoresis and SDS-PAGE. The results revealed intriguing differences in the behavior of bovine serum albumin (BSA) and other model proteins. Specifically, BSA exhibited Ferguson plot slopes that were dependent on the oligomer size in agarose native gel electrophoresis, while such size-dependent behavior was not observed in native-PAGE or SDS-PAGE. These findings suggest that Ferguson plot analysis is a suitable approach when using agarose gel under the electrophoretic conditions employed in this study. Furthermore, our investigation extended to model proteins with acidic isoelectric points and larger molecular weights, namely Ferritin and caseinolytic peptidase B (ClpB). Notably, these proteins displayed distinct Ferguson plot slopes when subjected to agarose gel electrophoresis. Intriguingly, when polyacrylamide gel was employed, ClpB exhibited multiple bands, each with its unique Ferguson plot slope, deviating from the expected behavior based on molecular size. This divergence in Ferguson plot characteristics between agarose and polyacrylamide gels points to an interesting and complex interplay between protein properties and gel electrophoresis conditions. Competing Interests: Declaration of competing interest This work was supported by Kyokuto Pharmaceutical Industrial Co., Ltd. Y.T., and T.A. (Teruo Akuta) are employees of the for-profit company Kyokuto Pharmaceuticals. M.T. is emeritus professor of the Kagoshima university and has no conflict of interest. T.A. (Tsutomu Arakawa) used to belong to the for-profit company Alliance Protein Laboratories but currently has no conflict of interest. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Agarose gel; Ferguson plot; Native gel electrophoresis; Polyacrylamide gel |
| المشرفين على المادة: | 9012-36-6 (Sepharose) 0 (polyacrylamide gels) 0 (Proteins) EC 3.4.21.92 (Endopeptidase Clp) 0 (Gels) 0 (Acrylic Resins) |
| تواريخ الأحداث: | Date Created: 20240217 Date Completed: 20240229 Latest Revision: 20240229 |
| رمز التحديث: | 20240229 |
| DOI: | 10.1016/j.bpc.2024.107200 |
| PMID: | 38367540 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4200 |
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| DOI: | 10.1016/j.bpc.2024.107200 |