دورية أكاديمية
Comprehensive analysis of antigenic variations and genomic properties of hepatitis B virus in clinical samples in the mid-north east region of Bangladesh.
| العنوان: | Comprehensive analysis of antigenic variations and genomic properties of hepatitis B virus in clinical samples in the mid-north east region of Bangladesh. |
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| المؤلفون: | Hossain MG; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address: mghossain@bau.edu.bd., Islam M; Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh., Araf Y; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Paul SK; Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh., Akter S; Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Khan MK; Department of Community Medicine, Mymensingh Medical College, Mymensingh, Bangladesh., Ahmed MU; Department of Medicine, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Khan S; Research Center for Global and Local Infectious Diseases, Oita University, Oita, Japan; Department of Microbiology, Faculty of Medicine, Oita University, Oita, Japan., Akbar SMF; Research Center for Global and Local Infectious Diseases, Oita University, Oita, Japan; Department of Gastroenterology and Metabology, Ehime University Graduate School of Medicine, Ehime, Japan; Miyakawa Memorial Research Foundation, Tokyo, Japan., Debnath CR; Department of Hepatology, Mymensingh Medical College, Mymensingh, Bangladesh. |
| المصدر: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Apr; Vol. 119, pp. 105572. Date of Electronic Publication: 2024 Feb 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 101084138 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1567-7257 (Electronic) Linking ISSN: 15671348 NLM ISO Abbreviation: Infect Genet Evol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| مواضيع طبية MeSH: | Carcinoma, Hepatocellular* , Hepatitis B* , Liver Neoplasms* , Hepatitis B, Chronic*, Humans ; Male ; Hepatitis B virus ; Hepatitis B Surface Antigens/genetics ; Bangladesh/epidemiology ; Phylogeny ; Reproducibility of Results ; Mutation ; Genotype ; Antigenic Variation ; Genomics ; DNA, Viral/genetics |
| مستخلص: | This investigation delineates an exhaustive analysis of the clinical, immunological, and genomic landscapes of hepatitis B virus (HBV) infection across a cohort of 22 verified patients. The demographic analysis unveiled a pronounced male bias (77.27%), with patient ages spanning 20 to 85 years and durations of illness ranging from 10 days to 4 years. Predominant clinical manifestations included fever, fatigue, anorexia, abdominal discomfort, and arthralgia, alongside observed co-morbidities such as chronic renal disorders and hepatocellular carcinoma. Antigenic profiling of the HBV envelope proteins elucidated significant heterogeneity among the infected subjects, particularly highlighted by discordances in the detection capabilities of small and large HBsAg assays, suggesting antigenic diversity. Quantitative assessment of viral loads unveiled a broad spectrum, accompanied by atypical HBeAg reactivity patterns, challenging the reliability of existing serological markers. Correlative studies between viral burden and antigenicity of the envelope proteins unearthed phenomena indicative of diagnostic evasion. Notably, samples demonstrating robust viral replication were paradoxically undetectable by the large HBsAg ELISA kit, advocating for more sophisticated diagnostic methodologies. Genotypic examination of three HBV isolates classified them as genotype D (D2), with phylogenetic alignment to strains from various global origins. Mutational profiling identified pivotal mutations within the basic core promoter and preS2/S1 regions, associated with an augmented risk of hepatocellular carcinoma. Further, mutations discerned in the small HBsAg and RT/overlap regions were recognized as contributors to vaccine and/or diagnostic escape mechanisms. In summation, this scholarly discourse elucidates the intricate interplay of clinical presentations, antigenic diversity, and genomic attributes in HBV infection, accentuating the imperative for ongoing investigative endeavors to refine diagnostic and therapeutic modalities. Competing Interests: Declaration of competing interest The authors affirm no known financial conflicts or personal relationships that might have influenced the work presented in this paper. (Copyright © 2024. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Antigenicity; Clinical features; Complete genome; Hepatitis B virus; Surface proteins |
| المشرفين على المادة: | 0 (Hepatitis B Surface Antigens) 0 (DNA, Viral) |
| تواريخ الأحداث: | Date Created: 20240217 Date Completed: 20240327 Latest Revision: 20240327 |
| رمز التحديث: | 20240327 |
| DOI: | 10.1016/j.meegid.2024.105572 |
| PMID: | 38367678 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1567-7257 |
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| DOI: | 10.1016/j.meegid.2024.105572 |