دورية أكاديمية
أعرض في EDS

Improvement of Mouse Preantral Follicle Survival and Development following Co-Culture with Ovarian Parenchyma Cell Suspension.

التفاصيل البيبلوغرافية
العنوان: Improvement of Mouse Preantral Follicle Survival and Development following Co-Culture with Ovarian Parenchyma Cell Suspension.
المؤلفون: Najafi Salehi J; Department of Basic Sciences and New Biological Technologies, Science and Culture University, Tehran, Iran.; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Eimani H; Department of Basic Sciences and New Biological Technologies, Science and Culture University, Tehran, Iran.Email: eimanih@royaninstitute.org., Shahverdi A; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.; Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Totonchi M; Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Fathi R; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Moosavi SA; Department of Lab Sciences, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran., Taher Mofrad SMJ; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Tahaei LS; Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
المصدر: International journal of fertility & sterility [Int J Fertil Steril] 2024 Feb 02; Vol. 18 (2), pp. 153-161. Date of Electronic Publication: 2024 Feb 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Royan Institute of Iran Country of Publication: Iran NLM ID: 101487941 Publication Model: Electronic Cited Medium: Print ISSN: 2008-076X (Print) Linking ISSN: 20080778 NLM ISO Abbreviation: Int J Fertil Steril Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Tehran : Royan Institute of Iran
مستخلص: Background: The parallel and continued improvements in both infertility treatment and the management of malignancy cases have brought to the forefront the potential for fertility preservation. Using ovarian follicular resources can effectively improve reproductive capacity and prevent infertility. The primary aim of this research was to try to generate an appropriate in vivo environment for the growth of the mouse follicles. Hence, the possible effects of the ovarian parenchyma cell suspension were explored on the growth and maturation of preantral follicles in vitro.
Materials and Methods: In this experimental study, ovarian parenchymal cells were mechanically dissociated from preantral follicles of 12-14 days-old NMRI mice and then divided into 5 experimental groups (G1: Control, G2: Fresh follicle with fresh parenchyma cell suspension, G3: Vitrified-warmed follicle with fresh parenchyma cell suspension, G4: Fresh follicle with frozen-thawed parenchyma cell suspension, and G5: Vitrified-warmed follicle with frozenthawed parenchyma cell suspension). The diameter of the follicles and immature oocytes, viability, antrum formation, resumption of meiosis, in vitro fertilization (IVF), and Gdf9, Bmp6, and Bmp15 gene expression were examined on different periods.
Results: The diameter of the follicles and the oocytes on days 4 and 8, as well as the survival rate of the follicles up to day 12, were significantly higher in G2 and G4 compared to the Ctrl group (G1: 73.66%, G2:87.99%, G3: 82.70%, G4: 94.37%, and G5: 78.59%). Expression of growth marker genes for G3, and G5 groups was significantly higher than other groups, which indicated the protective effects of parenchyma cell suspension on follicles damaged by vitrification solutions.
Conclusion: The growth, survival, and maturation of preantral follicles could be enhanced by co-culturing them with ovarian parenchyma cells. Further studies are needed to optimize the conditions for a successful parenchyma cell suspension-induced in vitro maturation (IVM) to occur in infertility clinics.
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فهرسة مساهمة: Keywords: Co-culture; Fertility Preservation; In Vitro Culture; Ovarian Tissue; Preantral Follicle
تواريخ الأحداث: Date Created: 20240218 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC10875308
DOI: 10.22074/ijfs.2023.1990372.1439
PMID: 38368519
قاعدة البيانات: MEDLINE
الوصف
تدمد:2008-076X
DOI:10.22074/ijfs.2023.1990372.1439