دورية أكاديمية
أعرض في EDS

Single-cell and spatial multi-omics highlight effects of anti-integrin therapy across cellular compartments in ulcerative colitis.

التفاصيل البيبلوغرافية
العنوان: Single-cell and spatial multi-omics highlight effects of anti-integrin therapy across cellular compartments in ulcerative colitis.
المؤلفون: Mennillo E; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Kim YJ; Chan Zuckerberg Biohub, San Francisco, CA, USA., Lee G; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Rusu I; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Patel RK; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA., Dorman LC; Chan Zuckerberg Biohub, San Francisco, CA, USA., Flynn E; CoLabs, University of California San Francisco, San Francisco, CA, USA., Li S; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Bain JL; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Andersen C; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA., Rao A; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA., Tamaki S; CoLabs, University of California San Francisco, San Francisco, CA, USA., Tsui J; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA.; Department of Pathology, University of California San Francisco, San Francisco, CA, 94143, USA., Shen A; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA.; Department of Pathology, University of California San Francisco, San Francisco, CA, 94143, USA., Lotstein ML; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA., Rahim M; Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA, 94143, USA., Naser M; Biological Imaging Development CoLab, University of California San Francisco, San Francisco, CA, USA., Bernard-Vazquez F; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Eckalbar W; CoLabs, University of California San Francisco, San Francisco, CA, USA., Cho SJ; Department of Pathology, University of California San Francisco, San Francisco, CA, 94143, USA., Beck K; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., El-Nachef N; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Lewin S; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Selvig DR; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Terdiman JP; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Mahadevan U; Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Oh DY; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; Division of Hematology/Oncology, Department of Medicine, University of California San Francisco, San Francisco, CA, USA., Fragiadakis GK; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA., Pisco A; Chan Zuckerberg Biohub, San Francisco, CA, USA., Combes AJ; Department of Medicine, University of California San Francisco, San Francisco, CA, USA.; CoLabs, University of California San Francisco, San Francisco, CA, USA.; Department of Pathology, University of California San Francisco, San Francisco, CA, 94143, USA., Kattah MG; Department of Medicine, University of California San Francisco, San Francisco, CA, USA. michael.kattah@ucsf.edu.
المصدر: Nature communications [Nat Commun] 2024 Feb 19; Vol. 15 (1), pp. 1493. Date of Electronic Publication: 2024 Feb 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Colitis, Ulcerative*/drug therapy , Colitis, Ulcerative*/genetics, Humans ; Integrins/genetics ; Multiomics ; Proteomics ; Gastrointestinal Agents/therapeutic use ; Treatment Outcome ; Retrospective Studies
مستخلص: Ulcerative colitis (UC) is driven by immune and stromal subsets, culminating in epithelial injury. Vedolizumab (VDZ) is an anti-integrin antibody that is effective for treating UC. VDZ is known to inhibit lymphocyte trafficking to the intestine, but its broader effects on other cell subsets are less defined. To identify the inflammatory cells that contribute to colitis and are affected by VDZ, we perform single-cell transcriptomic and proteomic analyses of peripheral blood and colonic biopsies in healthy controls and patients with UC on VDZ or other therapies. Here we show that VDZ treatment is associated with alterations in circulating and tissue mononuclear phagocyte (MNP) subsets, along with modest shifts in lymphocytes. Spatial multi-omics of formalin-fixed biopsies demonstrates trends towards increased abundance and proximity of MNP and fibroblast subsets in active colitis. Spatial transcriptomics of archived specimens pre-treatment identifies epithelial-, MNP-, and fibroblast-enriched genes related to VDZ responsiveness, highlighting important roles for these subsets in UC.
(© 2024. The Author(s).)
التعليقات: Update of: bioRxiv. 2024 Jan 03:2023.01.21.525036. doi: 10.1101/2023.01.21.525036. (PMID: 36711576)
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معلومات مُعتمدة: P30 DK063720 United States DK NIDDK NIH HHS; K08 DK123202 United States DK NIDDK NIH HHS; S10 OD025187 United States OD NIH HHS; P30 AR070155 United States AR NIAMS NIH HHS; S10 OD018040 United States OD NIH HHS
المشرفين على المادة: 0 (Integrins)
0 (Gastrointestinal Agents)
تواريخ الأحداث: Date Created: 20240219 Date Completed: 20240221 Latest Revision: 20240612
رمز التحديث: 20240612
مُعرف محوري في PubMed: PMC10876948
DOI: 10.1038/s41467-024-45665-6
PMID: 38374043
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-024-45665-6