دورية أكاديمية
The toxicity response of the electrochemical signal of the cell to the drug metabolized by the S9 system.
| العنوان: | The toxicity response of the electrochemical signal of the cell to the drug metabolized by the S9 system. |
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| المؤلفون: | Zhang J; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn.; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China., Fei C; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn., Qi S; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn., Fu J; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn., Zhou S; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn.; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China., Wang Z; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China.; College of Biology and Agriculture, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China., Li J; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn.; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China., Zhao Y; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn.; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China., Wu D; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P. R. China. lijinlian@jmsu.edu.cn.; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, China. |
| المصدر: | The Analyst [Analyst] 2024 Mar 11; Vol. 149 (6), pp. 1921-1928. Date of Electronic Publication: 2024 Mar 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society |
| مواضيع طبية MeSH: | Benzo(a)pyrene*/metabolism , Benzo(a)pyrene*/toxicity , Biochemical Phenomena*, Fluorenes/toxicity ; Guanine ; Mutagens |
| مستخلص: | The electrochemical detection method of cytotoxicity using intracellular purines as biomarkers has shown great potential for in vitro drug toxicity evaluation. However, no electrochemical detection system based on an in vitro drug metabolism mechanism has been devised. In this paper, electrochemical voltammetry was used to investigate the effect of the S9 system on the electrochemical behavior of HepG2 cells, and benzo[ a ]pyrene, fluoranthene, and pyrene were employed to investigate the sensitivity of electrochemical signals of cells to the cytotoxicity of drugs metabolized by the S9 system. The results showed that, within 8 h of exposure to the S9 system, the electrochemical signal of HepG2 cells at 0.7 V did not alter noticeably. The levels of xanthine, guanine, hypoxanthine, and adenine in the cells were not significantly altered. Compared with the absence of S9 system metabolism, benzo[ a ]pyrene and fluoranthene processed by the S9 system decreased the electrochemical signal of the cells in a dose-dependent manner, while pyrene did not change it appreciably. HPLC also revealed that benzo[ a ]pyrene and fluoranthene metabolized by the S9 system decreased the intracellular purine levels, whereas pyrene had no effect on them before and after S9 system metabolism. The cytotoxicity results of the three drugs examined by electrochemical voltammetry and MTT assay showed a strong correlation and good agreement. The S9 system had no effect on the intracellular purine levels or the electrochemical signal of cells. When the drug was metabolized by the S9 system, variations in cytotoxicity could be precisely detected by electrochemical voltammetry. |
| المشرفين على المادة: | 360UOL779Z (fluoranthene) 3417WMA06D (Benzo(a)pyrene) 0 (Fluorenes) 5Z93L87A1R (Guanine) 0 (Mutagens) |
| تواريخ الأحداث: | Date Created: 20240220 Date Completed: 20240312 Latest Revision: 20240312 |
| رمز التحديث: | 20240312 |
| DOI: | 10.1039/d4an00010b |
| PMID: | 38375539 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1364-5528 |
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| DOI: | 10.1039/d4an00010b |