دورية أكاديمية
RNA and protein biomarkers for detecting enhanced metabolic resistance to herbicides mesosulfuron-methyl and fenoxaprop-ethyl in black-grass (Alopecurus myosuroides).
العنوان: | RNA and protein biomarkers for detecting enhanced metabolic resistance to herbicides mesosulfuron-methyl and fenoxaprop-ethyl in black-grass (Alopecurus myosuroides). |
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المؤلفون: | Lowe C; Protecting Crops and the Environment, Rothamsted Research, Harpenden, UK., Onkokesung N; Agriculture, School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, UK., Goldberg A; Agriculture, School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, UK., Beffa R; Senior Scientific Consultant, Liederbach, Germany., Neve P; Protecting Crops and the Environment, Rothamsted Research, Harpenden, UK., Edwards R; Agriculture, School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, UK., Comont D; Protecting Crops and the Environment, Rothamsted Research, Harpenden, UK. |
المصدر: | Pest management science [Pest Manag Sci] 2024 Jun; Vol. 80 (6), pp. 2539-2551. Date of Electronic Publication: 2024 Feb 20. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
مواضيع طبية MeSH: | Herbicides*/pharmacology , Poaceae*/drug effects , Poaceae*/metabolism , Poaceae*/genetics , Herbicide Resistance*/genetics , Sulfonylurea Compounds*/pharmacology , Propionates*/pharmacology , Propionates*/metabolism , Biomarkers*/metabolism, Plant Proteins/metabolism ; Plant Proteins/genetics ; Oxazoles/pharmacology |
مستخلص: | Background: The evolution of non-target site resistance (NTSR) to herbicides leads to a significant reduction in herbicide control of agricultural weed species. Detecting NTSR in weed populations prior to herbicide treatment would provide valuable information for effective weed control. While not all NTSR mechanisms have been fully identified, enhanced metabolic resistance (EMR) is one of the better studied, conferring tolerance through increased herbicide detoxification. Confirming EMR towards specific herbicides conventionally involves detecting metabolites of the active herbicide molecule in planta, but this approach is time-consuming and requires access to well-equipped laboratories. Results: In this study, we explored the potential of using molecular biomarkers to detect EMR before herbicide treatment in black-grass (Alopecurus myosuroides). We tested the reliability of selected biomarkers to predict EMR and survival after herbicide treatments in both reference and 27 field-derived black-grass populations collected from sites across the UK. The combined analysis of the constitutive expression of biomarkers and metabolism studies confirmed three proteins, namely, AmGSTF1, AmGSTU2 and AmOPR1, as differential biomarkers of EMR toward the herbicides fenoxaprop-ethyl and mesosulfuron in black-grass. Conclusion: Our findings demonstrate that there is potential to use molecular biomarkers to detect EMR toward specific herbicides in black-grass without reference to metabolism analysis. However, biomarker development must include testing at both transcript and protein levels in order to be reliable indicators of resistance. This work is a first step towards more robust resistance biomarker development, which could be expanded into other herbicide chemistries for on-farm testing and monitoring EMR in uncharacterised black-grass populations. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. (© 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.) |
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معلومات مُعتمدة: | United Kingdom BB_ Biotechnology and Biological Sciences Research Council |
فهرسة مساهمة: | Keywords: enhanced metabolic resistance; fenoxaprop; herbicide resistance black‐grass; mesosulfuron; molecular biomarkers; non‐target site resistance |
المشرفين على المادة: | 0 (Herbicides) 7U20WEM458 (fenoxaprop ethyl) 0 (Sulfonylurea Compounds) 0 (Propionates) 0 (Biomarkers) 22L00R79A6 (mesosulfuron-methyl) 0 (Plant Proteins) 0 (Oxazoles) |
تواريخ الأحداث: | Date Created: 20240220 Date Completed: 20240513 Latest Revision: 20240513 |
رمز التحديث: | 20240513 |
DOI: | 10.1002/ps.7960 |
PMID: | 38375975 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1526-4998 |
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DOI: | 10.1002/ps.7960 |