دورية أكاديمية
Oocyte competence and gene expression in parthenogenetic produced embryos from repeat breeder and normally fertile buffaloes (Bubalus bubalis) raised in sub-humid tropical climate.
العنوان: | Oocyte competence and gene expression in parthenogenetic produced embryos from repeat breeder and normally fertile buffaloes (Bubalus bubalis) raised in sub-humid tropical climate. |
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المؤلفون: | Kumar S; Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil. Electronic address: satishhau@gmail.com., Chaves MS; Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil., Ferreira ACA; Laboratory of Oocytes and Preantral Follicles Manipulation, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil., Bezerra da Silva AF; Laboratory of Oocytes and Preantral Follicles Manipulation, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil., Pereira LMC; Post-Graduate Program in Veterinary Science, Faculty of Veterinary, State University of Ceará, 60714-903 Fortaleza, Brazil., Vale WG; Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil., Filho STR; Animal Reproduction Sector, Federal Rural University of Amazon, 66077-830 Belém, Brazil., Watanabe YF; Vitrogen, 14140-000 Cravinhos, Brazil., Melo LM; Molecular Genetics Research Unit, University Center Fametro (Unifametro), 60010-470 Fortaleza, Brazil., Figueirêdo Freitas VJ; Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State, University of Ceará, 60714-903 Fortaleza, Brazil. |
المصدر: | Animal reproduction science [Anim Reprod Sci] 2024 Mar; Vol. 262, pp. 107426. Date of Electronic Publication: 2024 Feb 01. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
مواضيع طبية MeSH: | Buffaloes*/genetics , Tropical Climate*, Female ; Animals ; Fertilization in Vitro/veterinary ; Oocytes/physiology ; Blastocyst/physiology ; Gene Expression ; In Vitro Oocyte Maturation Techniques/veterinary ; Embryonic Development/physiology |
مستخلص: | The reproductive management of the buffalo species still faces several unresolved problems, which directly affect the productivity of the herd, one of them being the presence of repeat breeder females. Given this scenario, this study aimed to verify the developmental competence of oocytes obtained from repeat breeder females and submitted to parthenogenetic activation. In addition, embryo gene expression was compared to normally fertile females. Murrah buffaloes were divided into two groups: repeat breeder (RB, n = 8) and normally fertile or control (CR, n = 7). Cumulus-oocyte complexes (COCs) were aspirated by transvaginal ovum pick-up from estrus synchronized females. The COCs were submitted to IVM for 24 h, and subsequently, the oocytes were activated using ionomycin, followed by 6-DMAP. Afterwards, the presumptive parthenotes were cultured for six or seven days in a microenvironment of 5 % CO Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
فهرسة مساهمة: | Keywords: Chemical activation; Embryo; Repeat breeding; Subfertility |
تواريخ الأحداث: | Date Created: 20240220 Date Completed: 20240301 Latest Revision: 20240301 |
رمز التحديث: | 20240301 |
DOI: | 10.1016/j.anireprosci.2024.107426 |
PMID: | 38377631 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1873-2232 |
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DOI: | 10.1016/j.anireprosci.2024.107426 |