دورية أكاديمية
A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.
العنوان: | A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear. |
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المؤلفون: | Goyal MM; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Shen SA; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Lehar M; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Martinez A; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Hiel H; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Wang C; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Liu Y; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Wang C; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Sun DQ; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.; Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. |
المصدر: | The Laryngoscope [Laryngoscope] 2024 Jul; Vol. 134 (7), pp. 3355-3362. Date of Electronic Publication: 2024 Feb 20. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8607378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4995 (Electronic) Linking ISSN: 0023852X NLM ISO Abbreviation: Laryngoscope Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2009- >: Philadelphia, PA : Wiley-Blackwell Original Publication: St. Louis, Mo. : [s.n., 1896- |
مواضيع طبية MeSH: | Round Window, Ear*/metabolism , Drug Delivery Systems*, Guinea Pigs ; Animals ; Ear, Inner/metabolism ; Magnetic Iron Oxide Nanoparticles/chemistry ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Magnetite Nanoparticles ; Printing, Three-Dimensional ; Polyethylene Glycols/chemistry |
مستخلص: | Introduction: The round window membrane (RWM) presents a significant barrier to the local application of therapeutics to the inner ear. We demonstrate a benchtop preclinical RWM model and evaluate superparamagnetic iron oxide nanoparticles (SPIONs) as vehicles for magnetically assisted drug delivery. Methods: Guinea pig RWM explants were inset into a 3D-printed dual chamber benchtop device. Custom-synthesized 7-nm iron core nanoparticles were modified with different polyethylene glycol chains to yield two sizes of SPIONs (NP-PEG600 and NP-PEG3000) and applied to the benchtop model with and without a magnetic field. Histologic analysis of the RWM was performed using transmission electron microscopy (TEM) and confocal microscopy. Results: Over a 4-h period, 19.5 ± 1.9% of NP-PEG3000 and 14.6 ± 1.9% of NP-PEG600 were transported across the guinea pig RWM. The overall transport increased by 1.45× to 28.4 ± 5.8% and 21.0 ± 2.0%, respectively, when a magnetic field was applied. Paraformaldehyde fixation of the RWM decreased transport significantly (NP-PEG3000: 7.6 ± 1.5%; NP-PEG600: 7.0 ± 1.6%). Confocal and electron microscopy analysis demonstrated nanoparticle localization throughout all cellular layers and layer-specific transport characteristics within RWM. Conclusion: The guinea pig RWM explant benchtop model allows for targeted and practical investigations of transmembrane transport in the development of nanoparticle drug delivery vehicles. The presence of a magnetic field increases SPION delivery by 45%-50% in a nanoparticle size- and cellular layer-dependent manner. Level of Evidence: NA Laryngoscope, 134:3355-3362, 2024. (© 2024 The American Laryngological, Rhinological and Otological Society, Inc.) |
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معلومات مُعتمدة: | K08 DC020780 United States DC NIDCD NIH HHS; K08DC020780 United States DC NIDCD NIH HHS; 5T32DC000027-33 United States DC NIDCD NIH HHS; K08DC020780 United States DC NIDCD NIH HHS; 5T32DC000027-33 United States DC NIDCD NIH HHS |
فهرسة مساهمة: | Keywords: drug delivery; ex vivo model; hearing loss; intratympanic delivery; nanoparticle transport; round window membrane; super‐paramagnetic iron oxide nanoparticles |
المشرفين على المادة: | 0 (Magnetite Nanoparticles) 3WJQ0SDW1A (Polyethylene Glycols) |
تواريخ الأحداث: | Date Created: 20240221 Date Completed: 20240617 Latest Revision: 20240617 |
رمز التحديث: | 20240617 |
DOI: | 10.1002/lary.31345 |
PMID: | 38379206 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1531-4995 |
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DOI: | 10.1002/lary.31345 |