دورية أكاديمية
أعرض في EDS

A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.

التفاصيل البيبلوغرافية
العنوان: A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.
المؤلفون: Goyal MM; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Shen SA; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Lehar M; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Martinez A; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Hiel H; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Wang C; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Liu Y; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA., Wang C; Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Sun DQ; Department of Chemical & Biomolecular Engineering, Johns Hopkins University, Baltimore, Maryland, USA.; Department of Otolaryngology-Head and Neck Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
المصدر: The Laryngoscope [Laryngoscope] 2024 Jul; Vol. 134 (7), pp. 3355-3362. Date of Electronic Publication: 2024 Feb 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8607378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-4995 (Electronic) Linking ISSN: 0023852X NLM ISO Abbreviation: Laryngoscope Subsets: MEDLINE
أسماء مطبوعة: Publication: <2009- >: Philadelphia, PA : Wiley-Blackwell
Original Publication: St. Louis, Mo. : [s.n., 1896-
مواضيع طبية MeSH: Round Window, Ear*/metabolism , Drug Delivery Systems*, Guinea Pigs ; Animals ; Ear, Inner/metabolism ; Magnetic Iron Oxide Nanoparticles/chemistry ; Microscopy, Confocal ; Microscopy, Electron, Transmission ; Magnetite Nanoparticles ; Printing, Three-Dimensional ; Polyethylene Glycols/chemistry
مستخلص: Introduction: The round window membrane (RWM) presents a significant barrier to the local application of therapeutics to the inner ear. We demonstrate a benchtop preclinical RWM model and evaluate superparamagnetic iron oxide nanoparticles (SPIONs) as vehicles for magnetically assisted drug delivery.
Methods: Guinea pig RWM explants were inset into a 3D-printed dual chamber benchtop device. Custom-synthesized 7-nm iron core nanoparticles were modified with different polyethylene glycol chains to yield two sizes of SPIONs (NP-PEG600 and NP-PEG3000) and applied to the benchtop model with and without a magnetic field. Histologic analysis of the RWM was performed using transmission electron microscopy (TEM) and confocal microscopy.
Results: Over a 4-h period, 19.5 ± 1.9% of NP-PEG3000 and 14.6 ± 1.9% of NP-PEG600 were transported across the guinea pig RWM. The overall transport increased by 1.45× to 28.4 ± 5.8% and 21.0 ± 2.0%, respectively, when a magnetic field was applied. Paraformaldehyde fixation of the RWM decreased transport significantly (NP-PEG3000: 7.6 ± 1.5%; NP-PEG600: 7.0 ± 1.6%). Confocal and electron microscopy analysis demonstrated nanoparticle localization throughout all cellular layers and layer-specific transport characteristics within RWM.
Conclusion: The guinea pig RWM explant benchtop model allows for targeted and practical investigations of transmembrane transport in the development of nanoparticle drug delivery vehicles. The presence of a magnetic field increases SPION delivery by 45%-50% in a nanoparticle size- and cellular layer-dependent manner.
Level of Evidence: NA Laryngoscope, 134:3355-3362, 2024.
(© 2024 The American Laryngological, Rhinological and Otological Society, Inc.)
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معلومات مُعتمدة: K08 DC020780 United States DC NIDCD NIH HHS; K08DC020780 United States DC NIDCD NIH HHS; 5T32DC000027-33 United States DC NIDCD NIH HHS; K08DC020780 United States DC NIDCD NIH HHS; 5T32DC000027-33 United States DC NIDCD NIH HHS
فهرسة مساهمة: Keywords: drug delivery; ex vivo model; hearing loss; intratympanic delivery; nanoparticle transport; round window membrane; super‐paramagnetic iron oxide nanoparticles
المشرفين على المادة: 0 (Magnetite Nanoparticles)
3WJQ0SDW1A (Polyethylene Glycols)
تواريخ الأحداث: Date Created: 20240221 Date Completed: 20240617 Latest Revision: 20240617
رمز التحديث: 20240617
DOI: 10.1002/lary.31345
PMID: 38379206
قاعدة البيانات: MEDLINE
الوصف
تدمد:1531-4995
DOI:10.1002/lary.31345