دورية أكاديمية
Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating β-cryptoxanthin in Citrus.
العنوان: | Cytochrome P450 CitCYP97B modulates carotenoid accumulation diversity by hydroxylating β-cryptoxanthin in Citrus. |
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المؤلفون: | Zhang Y; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Jin J; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Wang N; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Sun Q; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Feng D; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Zhu S; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Wang Z; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Li S; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Ye J; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Chai L; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Xie Z; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China., Deng X; National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China. Electronic address: xxdeng@mail.hzau.edu.cn. |
المصدر: | Plant communications [Plant Commun] 2024 Jun 10; Vol. 5 (6), pp. 100847. Date of Electronic Publication: 2024 Feb 19. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Published by the Plant Communications Shanghai Office in association with Cell Press, an imprint of Elsevier Inc Country of Publication: China NLM ID: 101769147 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2590-3462 (Electronic) Linking ISSN: 25903462 NLM ISO Abbreviation: Plant Commun Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Shanghai] : Published by the Plant Communications Shanghai Office in association with Cell Press, an imprint of Elsevier Inc., [2020]- |
مواضيع طبية MeSH: | Citrus*/genetics , Citrus*/metabolism , Beta-Cryptoxanthin*/metabolism , Cytochrome P-450 Enzyme System*/genetics , Cytochrome P-450 Enzyme System*/metabolism , Carotenoids*/metabolism, Hydroxylation ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Gene Expression Regulation, Plant ; Genome-Wide Association Study |
مستخلص: | Carotenoids in plant foods provide health benefits by functioning as provitamin A. One of the vital provitamin A carotenoids, β-cryptoxanthin, is typically plentiful in citrus fruit. However, little is known about the genetic basis of β-cryptoxanthin accumulation in citrus. Here, we performed a widely targeted metabolomic analysis of 65 major carotenoids and carotenoid derivatives to characterize carotenoid accumulation in Citrus and determine the taxonomic profile of β-cryptoxanthin. We used data from 81 newly sequenced representative accessions and 69 previously sequenced Citrus cultivars to reveal the genetic basis of β-cryptoxanthin accumulation through a genome-wide association study. We identified a causal gene, CitCYP97B, which encodes a cytochrome P450 protein whose substrate and metabolic pathways in land plants were undetermined. We subsequently demonstrated that CitCYP97B functions as a novel monooxygenase that specifically hydroxylates the β-ring of β-cryptoxanthin in a heterologous expression system. In planta experiments provided further evidence that CitCYP97B negatively regulates β-cryptoxanthin content. Using the sequenced Citrus accessions, we found that two critical structural cis-element variations contribute to increased expression of CitCYP97B, thereby altering β-cryptoxanthin accumulation in fruit. Hybridization/introgression appear to have contributed to the prevalence of two cis-element variations in different Citrus types during citrus evolution. Overall, these findings extend our understanding of the regulation and diversity of carotenoid metabolism in fruit crops and provide a genetic target for production of β-cryptoxanthin-biofortified products. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
References: | Int J Cancer. 2000 Oct 1;88(1):146-50. (PMID: 10962453) Mol Plant. 2018 Jan 8;11(1):58-74. (PMID: 28958604) J Exp Bot. 2021 Sep 30;72(18):6274-6290. (PMID: 34125891) J Agric Food Chem. 2015 Sep 30;63(38):8601-14. (PMID: 26329679) Fly (Austin). 2012 Apr-Jun;6(2):80-92. (PMID: 22728672) J Biol Chem. 1999 Nov 5;274(45):32351-9. (PMID: 10542276) Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943) J Integr Plant Biol. 2014 Sep;56(9):902-15. (PMID: 24942088) Br J Nutr. 2011 Jan;105(2):212-9. (PMID: 20807466) Bioinformatics. 2012 Sep 15;28(18):i333-i339. (PMID: 22962449) Bioinformatics. 2009 Jul 15;25(14):1754-60. (PMID: 19451168) Plant J. 2007 Sep;51(6):1126-36. (PMID: 17666025) Nat Genet. 2017 May;49(5):765-772. (PMID: 28394353) Genome Biol. 2019 Dec 19;20(1):291. (PMID: 31856913) Annu Rev Plant Biol. 2010;61:291-315. (PMID: 20192745) Brief Bioinform. 2021 Jul 20;22(4):. (PMID: 33126247) Nat Methods. 2023 Mar;20(3):408-417. (PMID: 36658279) Phytochemistry. 2010 Feb;71(2-3):168-78. (PMID: 19939422) Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18232-7. (PMID: 19011084) Nutr Rev. 2016 Feb;74(2):69-82. (PMID: 26747887) New Phytol. 2022 Apr;234(1):164-178. (PMID: 35048386) Cancer Lett. 2001 Dec 28;174(2):141-50. (PMID: 11689289) Nat Biotechnol. 2014 Jul;32(7):656-62. (PMID: 24908277) Genome Res. 2010 Sep;20(9):1297-303. (PMID: 20644199) Plant Biotechnol J. 2014 Jan;12(1):17-27. (PMID: 24034339) J Exp Bot. 2012 Jul;63(12):4403-17. (PMID: 22611233) Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Nov;1865(11):158652. (PMID: 32035228) Methods Enzymol. 2022;670:179-212. (PMID: 35871836) J Exp Bot. 2014 Jun;65(9):2545-56. (PMID: 24692648) Trends Genet. 2015 Dec;31(12):709-719. (PMID: 26603610) Trends Plant Sci. 2019 Dec;24(12):1075-1082. (PMID: 31377174) Bioinformatics. 2019 May 15;35(10):1786-1788. (PMID: 30321304) J Exp Bot. 2020 Feb 19;71(4):1306-1321. (PMID: 31728522) Bioinformatics. 2019 Nov 1;35(21):4453-4455. (PMID: 31070718) Front Plant Sci. 2021 Dec 10;12:787049. (PMID: 34956282) Bioinformatics. 2016 Apr 15;32(8):1220-2. (PMID: 26647377) Genome Biol. 2021 Jul 12;22(1):202. (PMID: 34253237) Nat Genet. 2010 Apr;42(4):348-54. (PMID: 20208533) Am J Hum Genet. 2011 Jan 7;88(1):76-82. (PMID: 21167468) Nat Plants. 2020 Feb;6(2):131-142. (PMID: 32055045) J Sci Food Agric. 2015 Jul;95(9):1786-94. (PMID: 25270992) Mol Hortic. 2022 Jan 21;2(1):3. (PMID: 37789426) Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Nov;1865(11):158664. (PMID: 32068105) Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14150-14157. (PMID: 32513704) BMC Plant Biol. 2007 Mar 02;7:11. (PMID: 17335571) BMC Plant Biol. 2012 Apr 18;12:50. (PMID: 22513258) Am J Clin Nutr. 2013 Nov;98(5):1200-8. (PMID: 24004891) Bioinformatics. 2011 Aug 1;27(15):2156-8. (PMID: 21653522) Plant Cell. 2020 May;32(5):1536-1555. (PMID: 32132132) Mol Biol Evol. 2015 Jan;32(1):244-57. (PMID: 25246699) Nat Protoc. 2022 Aug;17(8):1735-1761. (PMID: 35715522) Nature. 2018 Feb 15;554(7692):311-316. (PMID: 29414943) Mol Plant. 2019 Sep 2;12(9):1294-1307. (PMID: 31102783) Mol Aspects Med. 2005 Dec;26(6):459-516. (PMID: 16309738) Poult Sci. 2012 Feb;91(2):432-8. (PMID: 22252357) Genetics. 2017 May;206(1):429-438. (PMID: 28341652) BMC Plant Biol. 2016 Jun 29;16(1):148. (PMID: 27358074) Plant Cell. 2010 Oct;22(10):3348-56. (PMID: 20889914) Am J Hum Genet. 2007 Sep;81(3):559-75. (PMID: 17701901) BMC Genomics. 2013 Jul 08;14:457. (PMID: 23834441) Genome Res. 2009 Sep;19(9):1655-64. (PMID: 19648217) J Agric Food Chem. 2014 Dec 10;62(49):11892-900. (PMID: 25393127) Nat Plants. 2021 Jul;7(7):954-965. (PMID: 34140668) Nat Biotechnol. 2005 Apr;23(4):482-7. (PMID: 15793573) J Agric Food Chem. 2006 Jun 14;54(12):4397-406. (PMID: 16756373) Plant Cell Physiol. 2009 Mar;50(3):463-79. (PMID: 19147649) Arch Biochem Biophys. 2007 Feb 15;458(2):146-57. (PMID: 17196929) Mol Plant. 2018 Aug 6;11(8):1024-1037. (PMID: 29885473) J Agric Food Chem. 2007 Mar 21;55(6):2356-68. (PMID: 17300198) J Biosci Bioeng. 2019 Dec;128(6):755-765. (PMID: 31277909) Osteoporos Int. 2011 Jan;22(1):143-52. (PMID: 20480147) Plant Physiol. 2019 Mar;179(3):830-843. (PMID: 30361256) Hortic Res. 2022 Dec 30;10(3):uhac290. (PMID: 36938563) Hortic Res. 2022 Mar 14;9:uhac063. (PMID: 35611182) |
فهرسة مساهمة: | Keywords: CYP97B; carotenoids; cytochrome P450; hydroxylation; β-cryptoxanthin |
المشرفين على المادة: | 0 (Beta-Cryptoxanthin) 9035-51-2 (Cytochrome P-450 Enzyme System) 36-88-4 (Carotenoids) 0 (Plant Proteins) |
تواريخ الأحداث: | Date Created: 20240221 Date Completed: 20240611 Latest Revision: 20240630 |
رمز التحديث: | 20240630 |
مُعرف محوري في PubMed: | PMC11211522 |
DOI: | 10.1016/j.xplc.2024.100847 |
PMID: | 38379285 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2590-3462 |
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DOI: | 10.1016/j.xplc.2024.100847 |