دورية أكاديمية
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Diosgenin biosynthesis investigation in medicinal herb (Tribulus terrestris) by transcriptome analysis.

التفاصيل البيبلوغرافية
العنوان: Diosgenin biosynthesis investigation in medicinal herb (Tribulus terrestris) by transcriptome analysis.
المؤلفون: Tyagi P; Plant Molecular Biology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282005, India., Prasad M; Plant Molecular Biology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282005, India., Mathur S; Plant Molecular Biology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282005, India., Ranjan R; Plant Molecular Biology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, Agra-282005, India. Electronic address: rajivranjanbt@gmail.com.
المصدر: Gene [Gene] 2024 Jan 30; Vol. 893, pp. 147937. Date of Electronic Publication: 2023 Oct 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
مواضيع طبية MeSH: Tribulus*/genetics , Plants, Medicinal*/genetics , Diosgenin*, Databases, Factual ; Gene Expression Profiling
مستخلص: Next-generation sequencing (NGS) has revolutionized the analysis of specific genes, pathways, and their regulation in various species. Tribulus terrestris L., an annual medicinal herb of Zygophyllaceae family, has gained significant attention due to its diverse medicinal properties, including anti-inflammatory, antimicrobial, and anti-cancer effects. Diosgenin, a steroidal saponin, is the major bioactive compound responsible for the medicinal importance of T. terrestris. However, there is a paucity of information regarding the genes involved in the diosgenin biosynthetic pathway in T. terrestris. To address this gap, this study aimed to identify candidate genes associated with diosgenin biosynthesis through whole transcriptome profiling. A total of ∼7.9 GB of data, comprising 482 million reads, was obtained and assembled into 148,871 unigenes. Subsequently, functional annotations were assigned to 50 % of the unigenes using sequence similarity searches against the NCBI non-redundant (NR), Uniprot, KEGG, Pfam, GO, and COG databases, primarily based on Gene Ontology and KEGG-KAAS pathways. The majority of unigenes associated with the biosynthesis of the steroidal diosgenin backbone exhibited up-regulation in the fruit, leaf, and root tissues, except the SQE gene in root. The differential expression of selected genes was further validated through quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the study identified 21,026 unigenes related to transcription factors and 15,551 unigenes containing simple sequence repeats (SSR). Notably, di-nucleotide SSR motifs exhibited a high repeat frequency. These findings greatly enhance our understanding of the diosgenin biosynthesis pathway and provide a basis for future research in molecular investigation and metabolic engineering, specifically for boosting diosgenin content.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Diosgenin biosynthesis; Homology; SSRs; Transcription factor; Transcriptome analysis; Tribulus terrestris
المشرفين على المادة: K49P2K8WLX (Diosgenin)
تواريخ الأحداث: Date Created: 20240221 Date Completed: 20240222 Latest Revision: 20240222
رمز التحديث: 20240222
DOI: 10.1016/j.gene.2023.147937
PMID: 38381509
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0038
DOI:10.1016/j.gene.2023.147937