دورية أكاديمية
Cytogenetic and Genomic Characterization of a Novel Wheat-Tetraploid Thinopyrum elongatum 1BS⋅1EL Translocation Line with Stripe Rust Resistance.
| العنوان: | Cytogenetic and Genomic Characterization of a Novel Wheat-Tetraploid Thinopyrum elongatum 1BS⋅1EL Translocation Line with Stripe Rust Resistance. |
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| المؤلفون: | Wu D; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Zhao X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Xie Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Li L; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Li Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Zhu W; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Xu L; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Wang Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Zeng J; College of Resources, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Cheng Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Sha L; College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Fan X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Zhang H; College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Zhou Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China., Kang H; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. |
| المصدر: | Plant disease [Plant Dis] 2024 Jul; Vol. 108 (7), pp. 2065-2072. Date of Electronic Publication: 2024 Jun 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Phytopathological Society Country of Publication: United States NLM ID: 9882809 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0191-2917 (Print) Linking ISSN: 01912917 NLM ISO Abbreviation: Plant Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: St. Paul, Minn. : American Phytopathological Society, [1980]- |
| مواضيع طبية MeSH: | Triticum*/microbiology , Triticum*/genetics , Plant Diseases*/microbiology , Plant Diseases*/genetics , Plant Diseases*/immunology , Disease Resistance*/genetics , Tetraploidy* , Poaceae*/genetics , Poaceae*/microbiology , Translocation, Genetic* , Puccinia*/physiology, Chromosomes, Plant/genetics ; Basidiomycota/physiology ; Plant Breeding ; In Situ Hybridization, Fluorescence ; Polymorphism, Single Nucleotide/genetics ; Genome, Plant/genetics |
| مستخلص: | Stripe rust, caused by Puccinia striiformis f. sp. tritici , is a destructive wheat disease pathogen. Thinopyrum elongatum is a valuable germplasm including diploid, tetraploid, and decaploid with plenty of biotic and abiotic resistance. In a previous study, we generated a stripe rust-resistant wheat-tetraploid Th. elongatum 1E/1D substitution line, K17-841-1. To further apply the wild germplasm for wheat breeding, we selected and obtained a new homozygous wheat-tetraploid Th. elongatum translocation line, T1BS⋅1EL, using genomic in situ hybridization, fluorescence in situ hybridization (FISH), oligo-FISH painting, and the wheat 55K single nucleotide polymorphism genotyping array. The T1BS⋅1EL is highly resistant to stripe rust at the seedling and adult stages. Pedigree and molecular marker analyses revealed that the resistance gene was located on the chromosome arm 1EL of tetraploid Th . elongatum, tentatively named Yr1EL . In addition, we developed and validated 32 simple sequence repeat markers and two kompetitive allele-specific PCR assays that were specific to the tetraploid Th. elongatum chromosome arm 1EL to facilitate marker-assisted selection for alien 1EL stripe rust resistance breeding. This will help us explore and locate the stripe rust resistance gene mapping on the 1E chromosome and deploy it in the wheat breeding program. Competing Interests: The author(s) declare no conflict of interest. |
| فهرسة مساهمة: | Keywords: chromosome translocation line; molecular marker; stripe rust; tetraploid Thinopyrum elongatum |
| SCR Organism: | Puccinia striiformis |
| تواريخ الأحداث: | Date Created: 20240221 Date Completed: 20240715 Latest Revision: 20240715 |
| رمز التحديث: | 20240715 |
| DOI: | 10.1094/PDIS-12-23-2799-RE |
| PMID: | 38381966 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0191-2917 |
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| DOI: | 10.1094/PDIS-12-23-2799-RE |