دورية أكاديمية
أعرض في EDS

Exploiting DNA methylation in cassava under water deficit for crop improvement.

التفاصيل البيبلوغرافية
العنوان: Exploiting DNA methylation in cassava under water deficit for crop improvement.
المؤلفون: Silva Filho JLBD; Laboratório de Genética Molecular de Plantas, Departamento de Genética, Universidade Federal de Pernambuco, Recife, Brazil., Pestana RKN; Embrapa Mandioca e Fruticultura, Rua da Embrapa, Cruz das Almas, BA, Brazil., Silva Júnior WJD; Laboratório de Genética Molecular de Plantas, Departamento de Genética, Universidade Federal de Pernambuco, Recife, Brazil., Coelho Filho MA; Embrapa Mandioca e Fruticultura, Rua da Embrapa, Cruz das Almas, BA, Brazil., Ferreira CF; Embrapa Mandioca e Fruticultura, Rua da Embrapa, Cruz das Almas, BA, Brazil., de Oliveira EJ; Embrapa Mandioca e Fruticultura, Rua da Embrapa, Cruz das Almas, BA, Brazil., Kido EA; Laboratório de Genética Molecular de Plantas, Departamento de Genética, Universidade Federal de Pernambuco, Recife, Brazil.
المصدر: PloS one [PLoS One] 2024 Feb 22; Vol. 19 (2), pp. e0296254. Date of Electronic Publication: 2024 Feb 22 (Print Publication: 2024).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: DNA Methylation* , Manihot*/genetics, Vegetables ; Anxiety ; Candy
مستخلص: DNA methylation plays a key role in the development and plant responses to biotic and abiotic stresses. This work aimed to evaluate the DNA methylation in contrasting cassava genotypes for water deficit tolerance. The varieties BRS Formosa (bitter) and BRS Dourada (sweet) were grown under greenhouse conditions for 50 days, and afterwards, irrigation was suspended. The stressed (water deficit) and non-stressed plants (negative control) consisted the treatments with five plants per variety. The DNA samples of each variety and treatment provided 12 MethylRAD-Seq libraries (two cassava varieties, two treatments, and three replicates). The sequenced data revealed methylated sites covering 18 to 21% of the Manihot esculenta Crantz genome, depending on the variety and the treatment. The CCGG methylated sites mapped mostly in intergenic regions, exons, and introns, while the CCNGG sites mapped mostly intergenic, upstream, introns, and exons regions. In both cases, methylated sites in UTRs were less detected. The differentially methylated sites analysis indicated distinct methylation profiles since only 12% of the sites (CCGG and CCNGG) were methylated in both varieties. Enriched gene ontology terms highlighted the immediate response of the bitter variety to stress, while the sweet variety appears to suffer more potential stress-damages. The predicted protein-protein interaction networks reinforced such profiles. Additionally, the genomes of the BRS varieties uncovered SNPs/INDELs events covering genes stood out by the interactomes. Our data can be useful in deciphering the roles of DNA methylation in cassava drought-tolerance responses and adaptation to abiotic stresses.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Silva Filho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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تواريخ الأحداث: Date Created: 20240222 Date Completed: 20240226 Latest Revision: 20240226
رمز التحديث: 20240226
مُعرف محوري في PubMed: PMC10883565
DOI: 10.1371/journal.pone.0296254
PMID: 38386677
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0296254